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ET14135

CSHL Arabidopsis genetrap mutant lines

Original: ET14135

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET14135

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1716031 [Map]; Strand: c
      • AT5G05700.1 - [+] show detail - arginine-tRNA protein transferase 1
        Distance: -300; Region: promoter
      • AT5G05710.1 - [+] show detail - Pleckstrin homology (PH) domain superfamily protein
        Distance: 0; Region: exon1 (CDS)
      • AT5G05720.1 - [+] show detail - RNA-binding (RRM/RBD/RNP motifs) family protein
        Distance: -840; Region: promoter
    Flanking Genome Sequence: ET14135 - 3-prime edge 
    GGTAGAGGTA TTTTACCGAC CGTTACCGAC CGTTTTCATC CCTACCCAGA 
    ACGAACCGGC TGGCTCACGA AACAAGGAGA GTACATCAAG ACTTGGCGAC 
    GCCGATGGTT CGTGCTCAAA CAAGGGAAAC TCTTCTGGTT CAAAGACTCC 
    GACGTCACGC GTGTGTCACG CCCTCGCGGT GTCGTCCCCG TCGCATCGTG 
    CTTAACCGCT AAAGGAGCAG AGGATGTACT CAACAAGCAA AACGCGTTCG 
    AGCTATCGAC GAGAAATGAG ACTATGTACT TCATTGCTGA TTCGGAGAAG 
    GAGAAAGAAG ATTGGATCAA TTCCATTGGA CGTTCCATCG TGCAAAACTC 
    CAGATCCGTT ACCGATTCTG AGATCGTTGA TTACGATAAC AAACACTAAA 
    GTTTCTCTGT TTTTGTCTTT TGTCAAAGTG ATTATGATCA CAGCTTTCAT 
    TGATGGAGTC TTAAATGTAA ATCTTACAAA ATCAAGAAAT GAAAAGCTTT 
    GTCTCTGCTT GAAAACATGA ACTCATATCC GAAAAACATA TAATATAAAA 
    CCTAATTCTT CAAGGGCAAC GCTTCTGGTT CAAAGACTTA CATGGTTCTT 
    CAAACATGTA CTTACCAAAT ATGGTAACAG AAGCAATACC TTTTGCACGT 
    GATCAAATAC AATAACTTCG TCGTACAGAC TAAGATGCAT GTATGGAAAG 
    TTCCTCTCGT CATTAAGTGT CCTAAACCCT ACCCG

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1715970 [Map]; Strand: c
      • AT5G05700.1 - [+] show detail - arginine-tRNA protein transferase 1
        Distance: -239; Region: promoter
      • AT5G05710.1 - [+] show detail - Pleckstrin homology (PH) domain superfamily protein
        Distance: 0; Region: exon1 (CDS)
      • AT5G05720.1 - [+] show detail - RNA-binding (RRM/RBD/RNP motifs) family protein
        Distance: -901; Region: promoter
    Flanking Genome Sequence: ET14135 - 5-prime edge 
    CTTTCTCCCT GGTTCTGGGT TAGACCAGAA CTCTACGCCT CCGTAGTCTT 
    CGGAATTGTT GTTCTGACCG CCGATTACAG CTCGCCACAG GCTTGCCATC 
    TTCCGAAGAT TGAACTGGAG AATTAGGGTT TTGATCCAAA TCTGCCGATG 
    CTATCGAAAA TCTGTTTATC GATTCCATTA TTTTTTCGTG TTTATCTTTT 
    GCCCCAAAAT GTTTTTGATT TTCCATTTTC TTCCCTTAAT ATATTTATAA 
    TTACATAACA CCCCCTTCAT TTTGCCAATT GCATAACAGT CAGCAACAAT 
    GTTTTAATAT TGAAAAACAA AAGTCAAATG TGAAGGAAGA TTATTTCTTC 
    TTCTCCAAAC GGAAAGAGCT TAGCAAAATT CTTCTGAATG AATAATCGGC 
    GATTATGTCT TTGAAAAACG ATGCGAGTAG TAGCCACGAC GGCGGAAGCA 
    ACGGAGAAAG CGTAATCGAT GATCATGGCC GCCGTAGAAG CACTTGCGGC 
    TACTGTAAAT CCCCAGCTCG GTCCAGTATC TCTCACGGTT CGTTTAATTT 
    TGCTTTTCTT ACGGATT

AT5G05700.1

Model type
Protein coding
Short Description
arginine-tRNA protein transferase 1
Curator Summary
Encodes an arginyl-tRNA:protein transferase (ATE1), a component of the N-end rule pathway that targets protein degradation through the identity of the amino-terminal residue of specific protein substrates. Arabidopsis contains two ATE genes: At5g05700/ATE1, At3g11240/ATE2. Another component of the N-end rule pathway is At5g02310/PROTEOLYSIS6 (PRT6). PRT6 and ATE were shown to regulate seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination. Mutants of ATE1 also display delayed leaf senescence.
Computational Description
arginine-tRNA protein transferase 1 (ATE1); CONTAINS InterPro DOMAIN/s: Arginine-tRNA-protein transferase, N-terminal (InterPro:IPR007471), Arginine-tRNA-protein transferase 1, eukaryotic (InterPro:IPR017137), Arginine-tRNA-protein transferase, C-terminal (InterPro:IPR007472); BEST Arabidopsis thaliana protein match is: arginine-tRNA protein transferase 2 (TAIR:AT3G11240.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G05710.1

Model type
Protein coding
Short Description
Pleckstrin homology (PH) domain superfamily protein
Computational Description
Pleckstrin homology (PH) domain superfamily protein; CONTAINS InterPro DOMAIN/s: Pleckstrin homology-type (InterPro:IPR011993), Pleckstrin homology (InterPro:IPR001849); BEST Arabidopsis thaliana protein match is: pleckstrin homologue 1 (TAIR:AT2G29700.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G05720.1

Model type
Protein coding
Short Description
RNA-binding (RRM/RBD/RNP motifs) family protein
Computational Description
RNA-binding (RRM/RBD/RNP motifs) family protein; FUNCTIONS IN: RNA binding, nucleotide binding, nucleic acid binding; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; CONTAINS InterPro DOMAIN/s: RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: nucleotide binding;nucleic acid binding;RNA binding (TAIR:AT4G19610.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR