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ET14282

CSHL Arabidopsis genetrap mutant lines

Original: ET14282

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET14282

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 4695909 [Map]; Strand: w
      • AT5G14565.1 - [+] show detail - MIR398C; miRNA
        Distance: 0; Region: exon2 (NonCoding)
      • AT5G14570.1 - [+] show detail - high affinity nitrate transporter 2.7
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: ET14282 - 3-prime edge
    TATTTTACCG ACCGTTACCG ACCGTTTTCA TCCCTAGGCG GGAGGGATGA 
    TATCGGATGC GCTGGGGAAG AGATTCGGTA TGAGAGGGAG GCTGTGGGGG
    CTATGGATCG TGCAATCGGT GGCTGGGTTG TTGTGCGTGT TACTCGGACG
    AGTCAACTCG CTCTGGGGAT CAATCCTCGT CATGTGGGTC TTCTCTGTTT
    TCGTTCAAGC TGCTTCTGGC CTTGTATTTG GCGTGGTCCC TTTCGTCTCC
    ACGCGGTTAG TTTAAAGTCT ACCAATCCGG TTTTTGCTAA TAATTTCGGT
    TTGGTTTTAA TTTGGTTTTG TTTATAATGA CAGATCGTTA GGAGTGGTGG
    CGGGAATTAC GGGAAGCGGC GGTACGGTTG GTGCGGTGGT GACGCAGTTT
    CTGTTGTTTT CCGGTGATGA TGTTCGAAAA CAGAGAAGCA TTTCACTTAT
    GGGTTTGATG ACTTTTGTGT TTGCTCTTTC TGTTACATCA ATTTACTTTC
    CACAATGGGG TGGAATGTGT TGTGGGCCTT CGTCATCTTC CGAAGAAGAA
    GATATTTCTC GGGGACTCCT TGTAGAAGAC GAAGATGAAG AAGGTAAAGT
    GGTTAGTGGT AGTCTACGTC CCGTTTGTTG ATGCCGCATT TGAACTGCTG
    GCCCATTTGG CCAAAGCAAA AGCCATAGCA AAACATTAGT ATACTACTAA
    TAAG
  • 5 prime edge [+] show sequence

    Not mapped

    Flanking Genome Sequence: ET14282 - 5-prime edge
    GGGGGGGGGG GGGGGGGGGG GGGGGGGGGG GGGGGGGGGG GGGGGGGGGG 
    GGGGGGGGGG GGGGGGGGGG GGGGGGAGGG GGGGGGGGGG GGAGGGGGGG
    GAGGGGGGGG GGGGGGGGGG GAGGGGGGGG GGGGGGGAGG AGAGGGGGGG
    GGGGGGGGGG GGGGGGGGGG GGGGGGGGGG GGGGGGGGGG GGGGGGGGGG
    GGGG
  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 4695998 [Map]; Strand: w
      • AT5G14565.1 - [+] show detail - MIR398C; miRNA
        Distance: 0; Region: exon2 (NonCoding)
      • AT5G14570.1 - [+] show detail - high affinity nitrate transporter 2.7
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: ET14282 - 5-prime edge
    GTCGCGACGC AATGTTCGAT ATCCCGAAAC TCGCCGCGAT CGTCCCCGCC 
    GCCTCCAGAT TCACTCCAAA TCTCTCGTAG AAATATCCGG CGATCACGTT
    GTCCGTCATT

AT5G14565.1

Model type
miRNA
Short Description
MIR398C; miRNA
Curator Summary
Encodes a microRNA that targets both CSD and CytC oxidase family members. MicroRNAs are regulatory RNAs with a mature length of ~21-nucleotides that are processed from hairpin precursors by Dicer-like enzymes. MicroRNAs can negatively regulate gene expression by attenuating translation or by directing mRNA cleavage.Mature sequence: UGUGUUCUCAGGUCACCCCUG. Down-regulated by biotic and abiotic stress.
Link
InterPro Scan - TAIR

AT5G14570.1

Model type
Protein coding
Short Description
high affinity nitrate transporter 2.7
Curator Summary
Encodes ATNRT2.7, a nitrate transporter that controls nitrate content in seeds. Expression is detected in reproductive organs and peaks in seeds. Localized to the vacuolar membrane.
Computational Description
high affinity nitrate transporter 2.7 (NRT2.7); CONTAINS InterPro DOMAIN/s: Major facilitator superfamily (InterPro:IPR020846), Major facilitator superfamily MFS-1 (InterPro:IPR011701), Major facilitator superfamily, general substrate transporter (InterPro:IPR016196); BEST Arabidopsis thaliana protein match is: nitrate transporter2.5 (TAIR:AT1G12940.1); Has 7777 Blast hits to 7739 proteins in 1625 species: Archae - 132; Bacteria - 6743; Metazoa - 43; Fungi - 326; Plants - 261; Viruses - 0; Other Eukaryotes - 272 (source: NCBI BLink).
Link
InterPro Scan - TAIR