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ET14325

CSHL Arabidopsis genetrap mutant lines

Original: ET14325

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET14325

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 5650398 [Map]; Strand: w
      • AT2G13550.1 - [+] show detail - unknown protein; Has 23 Blast hits to 20 proteins in 10 species: Archae - 0; ...
        Distance: -601; Region: promoter
      • AT2G13560.1 - [+] show detail - NAD-dependent malic enzyme 1
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: ET14325 - 3-prime edge 
    TTTACCGACC GTTACCGACC GTTTTCATCC CTAGCTCAGA GTCCATAACA 
    TTAGGAGGAA GAAGACCTCT AAGATCTAAA CGATTTCTCT CCGTCATCGT 
    AAACGCAGTC CCCTAACATG AATCAAAATC AGATCCAGGT TCGTGTTCAC 
    ACTCAAAATG TGAGAAACTG AGTTTCAAGT TTTTCAAGTA CCTTGTTGAA 
    CCAAGGATCA TGGAGGATAT CGAGACCTTG TTTATGAACG ATGGTGGGAC 
    GGTGACCTTC CGATGTGGTG AAAGATCTGA CCGCGGATGA GTAAAGTATT 
    CTCCGGTGGA GAATTCGGCT GAGAGATGAT GAACTTAGCC GGAGCTTATT 
    GGCTATTCCC ATCGTTTAAC GGAATCTATG GAGAAGATTG TTCCGATCTG 
    GCTGGAGATG AGAGAAAGCA AGCTTACCTG TTTTTGACTC TCCGTAGTCT 
    CTCTTTTTTT TATT

  • 5 prime edge [+] show sequence

    1. Chromosome: 2; Position: 5650495 [Map]; Strand: w
      • AT2G13550.1 - [+] show detail - unknown protein; Has 23 Blast hits to 20 proteins in 10 species: Archae - 0; ...
        Distance: -698; Region: promoter
      • AT2G13560.1 - [+] show detail - NAD-dependent malic enzyme 1
        Distance: 0; Region: intron2 (CDS)
    Flanking Genome Sequence: ET14325 - 5-prime edge 
    TTTTTCGTTT TAGTAAGTTT TACTTGAGAA AACGTAGTTT ATTGTTTATG 
    TTGTTGCCTT AGTGGTTGAC ATGATTGATT TTGATATAAC AGTGGCTGAC 
    CTGAAGAGAT TAGAGGAGCG GAGCTAGAGA TGGACCTTCT GATCCTAATG 
    ATTTGGCTAA ATGGAGGATT CTTAATCGGT TACGTGACCG AAATGAGACT 
    ATGTACTATA AGGCGAACTC GTTTTCATTT TTTGGTCTAT TTGCTGTGAA 
    AGATTTTGGT TACTACGCTT GTTTTTAGTT TCTGAAGAGG TTATTTACTT 
    CGATTTTTAC GTTGCTTTTA GGTTTTGATT AACAATATTG AGGAGTATGC 
    GCCGATAGTC TATACTCCTA CGGTTGGTCT TGTCTGCCAG AACTACAGTG 
    GGTTGTTTAG GAGGCCAAGG GGAATGTATT TTAGTGCTGA AGATCGTGGT 
    GAAATGATGT CCATGGTTTT ACAACTGGCC AGCTGAGCA

AT2G13550.1

Model type
Protein coding
Computational Description
unknown protein; Has 23 Blast hits to 20 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 10; Fungi - 0; Plants - 4; Viruses - 0; Other Eukaryotes - 9 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G13560.1

Model type
Protein coding
Short Description
NAD-dependent malic enzyme 1
Curator Summary
Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the alpha family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the beta-type NAD-ME2 (At4g00570). NAD-ME1 transcript and protein levels are higher during the night than during the day.
Computational Description
NAD-dependent malic enzyme 1 (NAD-ME1); FUNCTIONS IN: in 7 functions; INVOLVED IN: response to salt stress, malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 2 (TAIR:AT4G00570.1); Has 9465 Blast hits to 9448 proteins in 2440 species: Archae - 143; Bacteria - 6328; Metazoa - 607; Fungi - 221; Plants - 457; Viruses - 0; Other Eukaryotes - 1709 (source: NCBI BLink).
Link
InterPro Scan - TAIR