ET5844

CSHL Arabidopsis genetrap mutant lines

Original: ET5844

Phenotypes

Morphological phenotypes

Original description

  • semi-sterile
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: ET5844

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 3862151 [Map]; Strand: c
      • AT3G12120.1 - [+] show detail - fatty acid desaturase 2
        Distance: 0; Region: intron1 (5UTR)
      • AT3G12120.2 - [+] show detail - fatty acid desaturase 2
        Distance: 0; Region: intron1 (5UTR)
    Flanking Genome Sequence: ET5844 - 3-prime edge 
    ATTTTACCGA CCGTTACCGA CCGTTTTCAT CCCTAAACAA AGCCAAGAAA 
    ACAGTTGAAG TGATGTATAA GCCACCAGAA TTTGTCGACA TCATATATCA 
    GATTTAGATA TTTAAACAGA AAATAAAATG TGACGACGGA TCTAGAAAAT 
    TTGCCCTGCA ACTGTAATCG CATCTGAATC AATCAAAGAG CATCTGATCC 
    TGAATTATTT TGATTCCAAC TTTTATCGTA AGGGGTTTAA GAGAATCTAT 
    AAAACTTTTG TTTTCCTTTT ATTATGTATT TTTACTATAA AAACCCAATA 
    GGAGTAAAAA ATCCAGGAAA GATTCAATAG TAGAAGCAGA TTTTTACCCA 
    GAACAAATAG CCAAAAAATG AATACATTAA CAAATAACAA TTTATGCTGN 
    TTTTTTTTTT TTTAAATTCC CCCCCCCCCC CCCAAAA

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 3862063 [Map]; Strand: c
      • AT3G12120.1 - [+] show detail - fatty acid desaturase 2
        Distance: 0; Region: intron1 (5UTR)
      • AT3G12120.2 - [+] show detail - fatty acid desaturase 2
        Distance: 0; Region: intron1 (5UTR)
    Flanking Genome Sequence: ET5844 - 5-prime edge 
    TTGTCAACTT GGTTTTCAAT ACGATTTGTG ATTTCGATCG CTGAATTTTT 
    AATACAAGCA AACTGATGTT AACCACAAGC AAGAGATGTG ACCTGCCTTA 
    TTAACATCGT ATTACTTACT ACTAGTCGTA TTCTCAACGC AATCGTTTTT 
    GTATTTCTCA CATTATGCCG CTTCTCTACT CTTTATTCCT TTTGGTCCAC 
    GCATTTTCTA TTTGTGGCAA TCCCTTTCAC AACCTGATTT CCCACTTTGG 
    ATCATTTGTC TGAAGACTCT CTTGAATCGT TACCACTTGT TTCTTGTGCA 
    TGCTCTGTTT TTTAGAATTA ATGATAAAAC TATTCCATAG TCTTGAGTTT 
    TCAGCTTGTT GATTCTTTTG CTTTTGGTTT TCTGCAGAAA CATGGGTGCA 
    GGTGGAAGAA TGCCGGTTCC TACTTCTTCC AAGAAATCGG AAACCGACAC 
    CACAAAGCGT GTGCCGTGCG AGAAACCGCC TTTCTCGGTG GGAGATCTGA 
    AGAAAGCAAT CCCGCCGCAT TGTTTCAAAC GCTCAATCCC TCGCTCTTTC 
    TCCTACCTTA TCAGTGACAT CATTATAGCC TCATGCTTCT ACTACGTCGC 
    CACCAATTAC T

AT3G12120.1

Model type
Protein coding
Short Description
fatty acid desaturase 2
Curator Summary
Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
Computational Description
fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G12120.2

Model type
Protein coding
Short Description
fatty acid desaturase 2
Curator Summary
Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
Computational Description
fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
Link
InterPro Scan - TAIR