ET5952

CSHL Arabidopsis genetrap mutant lines

Original: ET5952

Phenotypes

Morphological phenotypes

Original description

  • semi-sterile
Anatomical entity
/structure development stage
Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: ET5952

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 14539530 [Map]; Strand: w
      • AT2G34500.1 - [+] show detail - cytochrome P450, family 710, subfamily A, polypeptide 1
        Distance: 0; Region: exon1 (3UTR)
    Flanking Genome Sequence: ET5952 - 3-prime edge
    TTGTCTTTGT TTTGCCATGT CTTCACCATA TTTGTTTTGT GTTGTCTTCC 
    AGTGAACATC AGTGATGTTA CAACGTGTTT CATACTTGTA TTTCT
  • 5 prime edge [+] show sequence

    1. Chromosome: 2; Position: 14539682 [Map]; Strand: w
      • AT2G34500.1 - [+] show detail - cytochrome P450, family 710, subfamily A, polypeptide 1
        Distance: 0; Region: exon1 (3UTR)
    Flanking Genome Sequence: ET5952 - 5-prime edge
    ACTCGTGGGC CACTCACTTC GAGAGAACAT CACGAAAAGT GATAAATTCA 
    CACTGGTTTC TTTGACCACC AAGAATAAAT TTAAGAAAAA TCCAAAAAAT
    TTAGGAAAAG TTGGGATACT TTGCGACGCG CCTAGACAAG AACACGGTGC
    ACCCATCCTT GGGCGATATA GTAGGGCAGT ACACGATCTC ATCACAACCG
    TCTGATCGAA GCCTCTTGAA ATCCAACAAC GACGAGAACA TTGCAATGAA
    GAGTACGAGG TGGTTCAACG CGTAACGCTG GCCTACGCAT TGGTGAGGCC
    CCCATCCAAA AGCTAGGAAG TTGCGTTTGA ACACCTGGTC CTCTTGTCTT
    GTCTCGCTAA ACCGGTCAGG ATCAAACCGG TCCGGTTCAG TAAACCCTTG
    GAACGAGGAG TCGAAAACCG AAGGAAAGAC AATTGTACCT TTTGGGATAG
    TGTACGTTTC CGTGAGAGGG AAGTCTATAG CAGCGACGTG TGGGACCATA
    GTTGCAGGAG GCCTGTATCT AATGACCTCA CGCGCCACGG AGCGTGTATA
    CT

AT2G34500.1

Model type
Protein coding
Short Description
cytochrome P450, family 710, subfamily A, polypeptide 1
Curator Summary
Encodes a protein with C22-sterol desaturase activity. The enzyme was shown to catalyze in the presence of NADPH the conversion of β-sitosterol to stigmasterol, but not that of 24-epi-campesterol to brassicasterol (unlike CYP710A2).
Computational Description
cytochrome P450, family 710, subfamily A, polypeptide 1 (CYP710A1); FUNCTIONS IN: C-22 sterol desaturase activity, oxygen binding; INVOLVED IN: oxidation reduction; LOCATED IN: endomembrane system; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, conserved site (InterPro:IPR017972), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 710, subfamily A, polypeptide 2 (TAIR:AT2G34490.1); Has 25674 Blast hits to 25623 proteins in 1435 species: Archae - 46; Bacteria - 2777; Metazoa - 10394; Fungi - 4411; Plants - 7239; Viruses - 0; Other Eukaryotes - 807 (source: NCBI BLink).
Link
InterPro Scan - TAIR