ET5952

CSHL Arabidopsis genetrap mutant lines

Phenotypes

Morphological phenotypes

Original description

semi-sterile

Anatomical entity /structure development stage	Phenotypes
seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997)

Original: ET5952

Determination of insertion point(s)

3 prime edge [+] show sequence
1. Chromosome: 2; Position: 14539530 [Map]; Strand: w
  - AT2G34500.1 - [+] show detail - cytochrome P450, family 710, subfamily A, polypeptide 1
    Distance: 0; Region: exon1 (3UTR)
Flanking Genome Sequence: ET5952 - 3-prime edge
```
TTGTCTTTGT TTTGCCATGT CTTCACCATA TTTGTTTTGT GTTGTCTTCC 
AGTGAACATC AGTGATGTTA CAACGTGTTT CATACTTGTA TTTCT
```

5 prime edge [+] show sequence

Chromosome: 2; Position: 14539682 [Map]; Strand: w
- AT2G34500.1 - [+] show detail - cytochrome P450, family 710, subfamily A, polypeptide 1
  Distance: 0; Region: exon1 (3UTR)

ACTCGTGGGC CACTCACTTC GAGAGAACAT CACGAAAAGT GATAAATTCA 
CACTGGTTTC TTTGACCACC AAGAATAAAT TTAAGAAAAA TCCAAAAAAT 
TTAGGAAAAG TTGGGATACT TTGCGACGCG CCTAGACAAG AACACGGTGC 
ACCCATCCTT GGGCGATATA GTAGGGCAGT ACACGATCTC ATCACAACCG 
TCTGATCGAA GCCTCTTGAA ATCCAACAAC GACGAGAACA TTGCAATGAA 
GAGTACGAGG TGGTTCAACG CGTAACGCTG GCCTACGCAT TGGTGAGGCC 
CCCATCCAAA AGCTAGGAAG TTGCGTTTGA ACACCTGGTC CTCTTGTCTT 
GTCTCGCTAA ACCGGTCAGG ATCAAACCGG TCCGGTTCAG TAAACCCTTG 
GAACGAGGAG TCGAAAACCG AAGGAAAGAC AATTGTACCT TTTGGGATAG 
TGTACGTTTC CGTGAGAGGG AAGTCTATAG CAGCGACGTG TGGGACCATA 
GTTGCAGGAG GCCTGTATCT AATGACCTCA CGCGCCACGG AGCGTGTATA 
CT

AT2G34500.1

Model type: Protein coding
Short Description: cytochrome P450, family 710, subfamily A, polypeptide 1
Curator Summary: Encodes a protein with C22-sterol desaturase activity. The enzyme was shown to catalyze in the presence of NADPH the conversion of β-sitosterol to stigmasterol, but not that of 24-epi-campesterol to brassicasterol (unlike CYP710A2).
Computational Description: cytochrome P450, family 710, subfamily A, polypeptide 1 (CYP710A1); FUNCTIONS IN: C-22 sterol desaturase activity, oxygen binding; INVOLVED IN: oxidation reduction; LOCATED IN: endomembrane system; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, conserved site (InterPro:IPR017972), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 710, subfamily A, polypeptide 2 (TAIR:AT2G34490.1); Has 25674 Blast hits to 25623 proteins in 1435 species: Archae - 46; Bacteria - 2777; Metazoa - 10394; Fungi - 4411; Plants - 7239; Viruses - 0; Other Eukaryotes - 807 (source: NCBI BLink).
Link: InterPro Scan - TAIR