ET6089

CSHL Arabidopsis genetrap mutant lines

Phenotypes

Morphological phenotypes

Original description

semi-sterile

Anatomical entity /structure development stage	Phenotypes
seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997)

Original: ET6089

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

3 prime edge [+] show sequence
1. Chromosome: 1; Position: 7294151 [Map]; Strand: w
  - AT1G20930.1 - [+] show detail - cyclin-dependent kinase B2;2
    Distance: 0; Region: intron1 (CDS)
Flanking Genome Sequence: ET6089 - 3-prime edge
```
CTGAATATGA AATCTTTAGG GTTCCTTATC TTATCCGAAT ACTANAAAAT 
GTCAAAGTAG AAGCTCTGTG TTGTGATCTC AGTTTCCGCT ATTTTTGTGG 
TGATTGCATT TCTACTGAGA GATTATTGTT ATGATGATTA TCTTCTTCT
```

3 prime edge [+] show sequence

Chromosome: 2; Position: 19662938 [Map]; Strand: c
- AT2G48060.1 - [+] show detail - unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biolo...
  Distance: -321; Region: promoter
- AT2G48070.3 - [+] show detail - resistance to phytophthora 1
  Distance: 0; Region: exon1 (CDS)
- AT2G48070.1 - [+] show detail - resistance to phytophthora 1
  Distance: 0; Region: exon1 (CDS)
- AT2G48070.2 - [+] show detail - resistance to phytophthora 1
  Distance: 0; Region: exon1 (CDS)

CCATGGGGTC TCCTCTTCCA TAGCTCTCAC TTATGGCTTT CGCCATATAA 
GAAGATCCAC CTTCCGTATT TTCGCCACCT CTGACGGATT AGAACCCAAG 
GACGACCCGC CGGAATCTCC TCTCCCTTCC TCATCCTCTG CTCTCGGTAA 
AGATTTGAAA AAGGTCCGCC TTATTTCTAT ATCATTTACG ATTTTTCTGT 
TGAAACCATA GGAAGACTTG AAAATGAATA TGCACCATGT TAGATTGGTC 
TCTAGTGCTT GAACCTGTCG AAAGAGTTGC TCTAGCTTAT GACTTTTCGA 
TTCAAACAGG TTGTTAACAA GACTGCTGCA ACCTTTGCCC CTTGAGCTTC 
CACCGCCAGT AAAAACCCTG CTCTCCCTGG AACTACTCTT TACAGAGTTT 
TTGAGGTTCA AGGCTATGCA TCGATGTTCC TCGGAGGTGT CCTTTCTTTC 
AACCTCCTCT TTCCCTCCTT CGAACCTGAT TTATGGAGGT TGATGGGCAT 
GTGGTCCATT TGGATGTCCA GTATGTCTCC TTCTCTACTT TCTTCTAACT 
TTATTTATTC CAAGGCTGCC TTTTTGCCCC TAATCCTTTA CTTTCTTTCC 
ACCTATTCCT TCTCTTCGGG CAC

5 prime edge [+] show sequence

Chromosome: 1; Position: 23642505 [Map]; Strand: c
- AT1G63730.1 - [+] show detail - Disease resistance protein (TIR-NBS-LRR class) family
  Distance: 0; Region: exon2 (CDS)

CTTCAGAATC CTTAGAGATT GTAGTGTTTA GTTTGTTTGA TACATCTCTA 
GCAATCGTCT CAACCATCTT CGATTCCTTG TCCCTAACAA GAACGGAAAA 
TTCATAGAGT TTATCAAATA GTCATGTGAA ATGTTGTAAC TTCTATTGTA 
GAAATCTGAA CCTATCTTTA TTACGTAGTT AGCTAAAATA TTGTTTTTCA 
CATCAAAGAC AGGAGTCCTA TGTTGCTAAT ACAGTAACTC ATGAACCTAT 
CACATCTTTT AGATTCACAA AGAAAATTTC CTTAATCGGA TTTAAGAAGG 
CATGAATTCC AAATTAATTG AACGAAATCA AACAAACCAG TTTAAGAAGT 
GCTCTCCGGC TATGTTTCCC ACATCGTTCA AAGCTTGGCT CCATTTTTGC 
TTCTCCTCCT CCGTTTTACG AGCACAAGTT TCACTGAATC GGATCCCAAA 
TTCTCCGGTT TGTTTACGCA CATCAGATGG ATCAACTCCG TAAAAGACAG 
TCATCACTAT CTGCCCAATA TCTTCCTTGC ACTTCAAAAT CTCCAACAGC 
TCATCCAAAC ACCA

5 prime edge [+] show sequence
1. Chromosome: 1; Position: 23642471 [Map]; Strand: c
  - AT1G63730.1 - [+] show detail - Disease resistance protein (TIR-NBS-LRR class) family
    Distance: 0; Region: intron1 (CDS)
Flanking Genome Sequence: ET6089 - 5-prime edge
```
GTTTGATACA TCTCTAGCAA TCGTCTCAAC CATCTTCGAT TCCTTGTCCC 
TAACAAGGAA CGGAAAATTC ATAGAGTTTA TCAA
```

AT1G20930.1

Model type: Protein coding
Short Description: cyclin-dependent kinase B2;2
Curator Summary: Cyclin-dependent kinase, expressed in flowers and suspension cell culture, expression peaks during M phase in synchronized cultures. Required for proper organization of the shoot apical meristem and for hormone signaling. Expressed in the shoot apical meristem. Involved in regulation of the G2/M transition of the mitotic cell cycle.
Computational Description: cyclin-dependent kinase B2;2 (CDKB2;2); CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), Protein kinase, catalytic domain (InterPro:IPR000719), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271); BEST Arabidopsis thaliana protein match is: cyclin-dependent kinase B2;1 (TAIR:AT1G76540.1); Has 109034 Blast hits to 107606 proteins in 3051 species: Archae - 93; Bacteria - 11427; Metazoa - 41458; Fungi - 12141; Plants - 24398; Viruses - 437; Other Eukaryotes - 19080 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G63730.1

Model type: Protein coding
Short Description: Disease resistance protein (TIR-NBS-LRR class) family
Computational Description: Disease resistance protein (TIR-NBS-LRR class) family; FUNCTIONS IN: transmembrane receptor activity, nucleoside-triphosphatase activity, nucleotide binding, ATP binding; INVOLVED IN: defense response; LOCATED IN: intrinsic to membrane; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), NB-ARC (InterPro:IPR002182), Toll-Interleukin receptor (InterPro:IPR000157), Disease resistance protein (InterPro:IPR000767); BEST Arabidopsis thaliana protein match is: Disease resistance protein (TIR-NBS-LRR class) family (TAIR:AT1G63740.1); Has 17590 Blast hits to 13527 proteins in 497 species: Archae - 17; Bacteria - 935; Metazoa - 1465; Fungi - 25; Plants - 14797; Viruses - 0; Other Eukaryotes - 351 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT2G48060.1

Model type: Protein coding
Computational Description: unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: shoot, sperm cell; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3595 (InterPro:IPR021999); Has 533 Blast hits to 341 proteins in 95 species: Archae - 2; Bacteria - 15; Metazoa - 318; Fungi - 0; Plants - 93; Viruses - 0; Other Eukaryotes - 105 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT2G48070.1

Model type: Protein coding
Short Description: resistance to phytophthora 1
Curator Summary: Encodes a chloroplast protein RPH1 (resistance to Phytophthora 1). Involved in immune response to Phytophthora brassicae. rph1 mutant plants (in Ws background) are susceptible to P. brassicae.
Computational Description: RESISTANCE TO PHYTOPHTHORA 1 (RPH1); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: defense response to oomycetes, positive regulation of hydrogen peroxide biosynthetic process; LOCATED IN: chloroplast, plastid; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 47 Blast hits to 47 proteins in 22 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 6 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT2G48070.2

Model type: Protein coding
Short Description: resistance to phytophthora 1
Curator Summary: Encodes a chloroplast protein RPH1 (resistance to Phytophthora 1). Involved in immune response to Phytophthora brassicae. rph1 mutant plants (in Ws background) are susceptible to P. brassicae.
Computational Description: RESISTANCE TO PHYTOPHTHORA 1 (RPH1); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: defense response to oomycetes, positive regulation of hydrogen peroxide biosynthetic process; LOCATED IN: chloroplast, plastid; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; Has 47 Blast hits to 47 proteins in 22 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 6 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT2G48070.3

Model type: Protein coding
Short Description: resistance to phytophthora 1
Curator Summary: Encodes a chloroplast protein RPH1 (resistance to Phytophthora 1). Involved in immune response to Phytophthora brassicae. rph1 mutant plants (in Ws background) are susceptible to P. brassicae.
Computational Description: RESISTANCE TO PHYTOPHTHORA 1 (RPH1); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: defense response to oomycetes, positive regulation of hydrogen peroxide biosynthetic process; LOCATED IN: plastid; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages.
Link: InterPro Scan - TAIR