ET6517

CSHL Arabidopsis genetrap mutant lines

Original: ET6517

Phenotypes

Morphological phenotypes

Original description

  • small siliques, very few seed, semi-sterile
Anatomical entity
/structure development stage
Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
fruit (PO:0009001)
  • decreased size (PATO:0000587)

Original: ET6517

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 28453948 [Map]; Strand: c
      • AT1G75780.1 - [+] show detail - tubulin beta-1 chain
        Distance: -308; Region: promoter
    Flanking Genome Sequence: ET6517 - 3-prime edge
    GTAGAGGTAT TTTACCGACC GTTACCGACC GTTTTCATCC CTAATTATAT 
    CGTCCATTTA ACTATTTAAG TGTGATAGCT TTCAAGTATC GATCATTCAT
    TCTAGTTTCA AATCTTGATC CATTTGATGA ATCTTTTACT ATTCGAATTT
    CATCACGACT AGCTACAACC ACCCTTGATT ATATGTTATG ACTTCTGAAA
    ACATCGAGAA ACAAAAATTC AATAATAGTG CGTAGAAGAA GATGATCAAT
    GCGTAATGAA TACAGTAACT TTGCATGTAG TATCTTAGTT GTTTTAGTGA
    CATAGCACAT GTAGCAAGAC GTAATAAACG TTCTGGAGAT CTTGAATGCA
    TATGTACCTA TTTCTATGTA AACGATAAAA CACAATAATT GAAATCGGTT
    GAATGATCAA AAGTATAGAA AAATAGCATC ACACTGATTG TGTTTGGATC
    TATTATACAA TATTGATGAT AACATGAAAA ACAAATAATG CCATAGCACA
    TGTAGCTAGG TAGATGTAGA CTGTAGTAAG ATCTGAGATT TGATCCAATT
    ATTTTTTCAT TTTA
  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 12839245 [Map]; Strand: c
      • AT4G24972.1 - [+] show detail - tapetum determinant 1
        Distance: 0; Region: intron3 (CDS)
    Flanking Genome Sequence: ET6517 - 5-prime edge
    CTTTTCTCCA ATTCTCTCCG GGTCTACACT CCTTTCTGGT TTTCCTTTAT 
    ATAAACAATC ACACACACAA TCAAAAC
  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 17181257 [Map]; Strand: c
      • AT4G36360.1 - [+] show detail - beta-galactosidase 3
        Distance: 0; Region: exon1 (5UTR)
      • AT4G36360.2 - [+] show detail - beta-galactosidase 3
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: ET6517 - 5-prime edge
    TCCTACTTTC TCCCTGGTGA TGATGCGAGT AAGAGACACT TGTGAAGTGC 
    AATAAAACCC CCTTCTTCTT CCTCTCTTTC TCCTTCGTCT TCTTCTTCTT
    CTTCCTTCCT TCCTTCTCTC TCCTTATTAA GAAAGACCAA CACAGACACG
    GGAAGAAGTA GCTTGG
  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 28453642 [Map]; Strand: c
      • AT1G75780.1 - [+] show detail - tubulin beta-1 chain
        Distance: -2; Region: promoter
    Flanking Genome Sequence: ET6517 - 5-prime edge
    ACTTCCCAAA AAAAACTCTC CCGCCTCTCT CTCTCCCTCT CCCTTCTTAT 
    TCTCCTCCT
  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 28453871 [Map]; Strand: c
      • AT1G75780.1 - [+] show detail - tubulin beta-1 chain
        Distance: -231; Region: promoter
    Flanking Genome Sequence: ET6517 - 5-prime edge
    TAATTCAACA ATATTCTTAA ATAACATTAA TCATTCACTT TGTAGAAATT 
    TCAATTTGAC GGTGCTAACA ACAATATGTG ACATTTTTGT TTGCAGCAAA
    AAAGTCAAAT GGTAAAAAAA AACATTTTTT TAAAAAGAGA GTATAGTAAA
    AAAACAGAGG ATAAAAAACG AACAAACGGT TAAAGCTGGC AAAGATCCCA
    TCTTCCCCAC GCTGTCAAAT TTTGTCACTA CTTCCCAAAA AAAACTCTCC
    CGCCTCTCTC TCTCCCTCTC CCTT

AT1G75780.1

Model type
Protein coding
Short Description
tubulin beta-1 chain
Curator Summary
beta tubulin gene downregulated by phytochrome A (phyA)-mediated far-red light high-irradiance and the phytochrome B (phyB)-mediated red light high-irradiance responses
Computational Description
tubulin beta-1 chain (TUB1); FUNCTIONS IN: structural molecule activity, GTP binding, GTPase activity; INVOLVED IN: response to light stimulus, unidimensional cell growth; LOCATED IN: vacuole; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Beta tubulin (InterPro:IPR002453), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Beta tubulin, autoregulation binding site (InterPro:IPR013838), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin beta-5 chain (TAIR:AT1G20010.1); Has 23671 Blast hits to 23575 proteins in 4891 species: Archae - 36; Bacteria - 42; Metazoa - 4470; Fungi - 14216; Plants - 1549; Viruses - 0; Other Eukaryotes - 3358 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G24972.1

Model type
Protein coding
Short Description
tapetum determinant 1
Curator Summary
Encodes a novel small protein which is similar to proteins of unknown function from other plant species. TPD1 is involved in cell specification during anther and pollen development. Identified in a screen for male steriles. Mutants lack tapetal cells and have an increased number of microsporocytes. Expressed in flower buds, leaves and young seedlings. In anthers, TPD1 is expressed throughout pollen development in parietal cells and sporocytes. Physically interacts with the LRR kinase EMS1 and that interaction results in phosphorylation of TPD1.
Computational Description
TAPETUM DETERMINANT 1 (TPD1); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: cell fate determination, anther development; LOCATED IN: endomembrane system; EXPRESSED IN: microsporocyte, floral meristem, flower, anther, leaf; EXPRESSED DURING: seedling growth; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G32583.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G36360.1

Model type
Protein coding
Short Description
beta-galactosidase 3
Curator Summary
putative beta-galactosidase (BGAL3 gene)
Computational Description
beta-galactosidase 3 (BGAL3); FUNCTIONS IN: beta-galactosidase activity; INVOLVED IN: lactose catabolic process, using glucoside 3-dehydrogenase, carbohydrate metabolic process, lactose catabolic process via UDP-galactose, lactose catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 35, conserved site (InterPro:IPR019801), Glycoside hydrolase, family 35 (InterPro:IPR001944), D-galactoside/L-rhamnose binding SUEL lectin (InterPro:IPR000922), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Galactose-binding domain-like (InterPro:IPR008979), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta galactosidase 1 (TAIR:AT3G13750.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G36360.2

Model type
Protein coding
Short Description
beta-galactosidase 3
Curator Summary
putative beta-galactosidase (BGAL3 gene)
Computational Description
beta-galactosidase 3 (BGAL3); FUNCTIONS IN: beta-galactosidase activity; INVOLVED IN: carbohydrate metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 35, conserved site (InterPro:IPR019801), Glycoside hydrolase, family 35 (InterPro:IPR001944), D-galactoside/L-rhamnose binding SUEL lectin (InterPro:IPR000922), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Galactose-binding domain-like (InterPro:IPR008979); BEST Arabidopsis thaliana protein match is: beta galactosidase 1 (TAIR:AT3G13750.1); Has 2405 Blast hits to 2192 proteins in 479 species: Archae - 15; Bacteria - 1021; Metazoa - 436; Fungi - 211; Plants - 628; Viruses - 0; Other Eukaryotes - 94 (source: NCBI BLink).
Link
InterPro Scan - TAIR