ET7736

CSHL Arabidopsis genetrap mutant lines

Phenotypes

Morphological phenotypes

Original description

embryo lethal

Anatomical entity /structure development stage	Phenotypes
seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997)

Original: ET7736

Determination of insertion point(s)

3 prime edge [+] show sequence

Chromosome: 2; Position: 321048 [Map]; Strand: w
- AT2G01720.1 - [+] show detail - Ribophorin I
  Distance: -784; Region: promoter
- AT2G01730.1 - [+] show detail - cleavage and polyadenylation specificity factor 73 kDa subunit-II
  Distance: 0; Region: exon3 (CDS)

GTATTTTACC GACCGTTACC GACCGTTTTC ATCCCTANAC TCACCGACAT 
ATAAATCGGA CCATTGTACC CACAAACTTC CGTGAAGTAA GGAAGCGCTC 
CAACATGATC CATATGGCTA TTAACACTCA CAATTAAAAC CCACAAAAAC 
TAAAAAAGTG AATTTTAAAA AAGGGACAAA TTCTGGGTTT GAAGAATCAA 
TACAAGTGTG TGATGATGAT ACAAGAGATC GCGTTGTCAA AATCACCGGA 
TTTAGATATG AGAGAAAAAT CTGGGTAACG ATTGTGATCA TCACAGCCCA 
TATGCATCCC ACAATCGAAC ATTATCTTTT TACCATTAAT CGTCACTACC 
ACACAGCTCT TTCCAATCTC TTGTCCAGCA CCTGGATACA ACACAAAAAA 
GATAGAATAC TCTTATACAG AAATTGAAAT TGTGAAACTT TGAAGAAGCA 
AAGAGAGAAG AAGAAGAAGA ACAACAAAAA CTGTTACCAA GTACGAGACA 
ATCGATAGCC ATAGTTACTG AAGAAATAAA C

3 prime edge [+] show sequence
1. Chromosome: 2; Position: 320934 [Map]; Strand: w
  - AT2G01720.1 - [+] show detail - Ribophorin I
    Distance: -670; Region: promoter
  - AT2G01730.1 - [+] show detail - cleavage and polyadenylation specificity factor 73 kDa subunit-II
    Distance: 0; Region: intron2 (CDS)
Flanking Genome Sequence: ET7736 - 3-prime edge
```
TGGCTATTAA CACTCACAAT TAAAACCCAC AAAAACTAAA AAAGGGGATG 
TTAAAAAAGG GACAAATTCT GGGTTTGAAG AATCAATACA AGTGTGTGAT 
GATGATACAA GAGATCGCGT TGTCAAAATC ACCCGATTTA GATATGAGAG 
AAAAATCTGG GTAACCATTG TGATCATCAC AGCCCATATG CATCCCACAA 
TCCAACATTA TCTTTTTACC ATTAATCGTC ACTAC
```

5 prime edge [+] show sequence

Not mapped

CCTTTGGTTT TTTTTTTTTT TGGGGGGGCC CCATTAATTT AAAACCAACC 
ATTTAAAGTG GTTAAAAATT TTAAAACCCG GGTTTTTTTT CCAGGAAATT 
TAAAAATTTC TTGGGGGTTT AAAAAAATTT TTTAATTTTT TTTTTTCAAA 
AGTTTTTGTT TTTTTAAAAC TTTTTTTTAA AAGAAAAAAA AGG

AT2G01720.1

Model type: Protein coding
Short Description: Ribophorin I
Computational Description: Ribophorin I; FUNCTIONS IN: oligosaccharyl transferase activity, dolichyl-diphosphooligosaccharide-protein glycotransferase activity; INVOLVED IN: protein amino acid glycosylation; LOCATED IN: endoplasmic reticulum, plasma membrane, plant-type cell wall; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ribophorin I (InterPro:IPR007676); BEST Arabidopsis thaliana protein match is: Ribophorin I (TAIR:AT1G76400.1); Has 386 Blast hits to 386 proteins in 178 species: Archae - 0; Bacteria - 0; Metazoa - 143; Fungi - 127; Plants - 63; Viruses - 0; Other Eukaryotes - 53 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT2G01730.1

Model type: Protein coding
Short Description: cleavage and polyadenylation specificity factor 73 kDa subunit-II
Curator Summary: a homolog of cleavage and polyadenylation specificity factor that plays an essential role in the development of female gametophyte and embryo
Computational Description: cleavage and polyadenylation specificity factor 73 kDa subunit-II (CPSF73-II); CONTAINS InterPro DOMAIN/s: RNA-metabolising metallo-beta-lactamase (InterPro:IPR011108), Beta-Casp domain (InterPro:IPR022712), Beta-lactamase-like (InterPro:IPR001279); BEST Arabidopsis thaliana protein match is: cleavage and polyadenylation specificity factor 73-I (TAIR:AT1G61010.3); Has 5072 Blast hits to 5039 proteins in 1435 species: Archae - 491; Bacteria - 2753; Metazoa - 569; Fungi - 259; Plants - 193; Viruses - 0; Other Eukaryotes - 807 (source: NCBI BLink).
Link: InterPro Scan - TAIR