ET977

CSHL Arabidopsis genetrap mutant lines

Phenotypes

Morphological phenotypes

Original description

semi-sterile

Anatomical entity /structure development stage	Phenotypes
seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997)

Original: ET977

Determination of insertion point(s)

3 prime edge [+] show sequence

Chromosome: 4; Position: 10435 [Map]; Strand: w
- AT4G00020.2 - [+] show detail - BREAST CANCER 2 like 2A
  Distance: -71; Region: promoter
- AT4G00020.1 - [+] show detail - BREAST CANCER 2 like 2A
  Distance: 0; Region: exon1 (5UTR)

GTCNGNTGNG TTNGTGTTTT CATCGCTCCC GTGTGGTAGA GCAATTCTGA 
ATCGCGAGGT TTAGTTCTCC GGCTGAATCA GAGAACGACG NGTCGGAAAA 
ATTCGAAACC CTAGAACTGA ATAAATGTCG ACGTGGCAAT TATTTCCCGA 
TTCCAGCGGC GATGGTTTCC GGTGGGAAGT CGCTGGCCGG ATTCTTCAGT 
CCGTCTCGGA TTCTACGCCA ACCAAGGCGC TCGAATCGAC AGCTCCTCTA 
CCTTCTATGG CCGATCTCTT GCTCCAAGGT GATTCTTCGT TTCTTCCTTT 
TTTTGTCGAA TCGTGTTTGT CATGTGACTC AAAGGAGATT GAATTATTAT 
TTGATGCTTC AAGGATGCTC GAAGCTTATA GAACGGGATA AATNCGGTNN 
TNGNGANNTG GGANTGTTCN GGANTGGGN

3 prime edge [+] show sequence
1. Chromosome: 4; Position: 10401 [Map]; Strand: w
  - AT4G00020.2 - [+] show detail - BREAST CANCER 2 like 2A
    Distance: -37; Region: promoter
  - AT4G00020.1 - [+] show detail - BREAST CANCER 2 like 2A
    Distance: 0; Region: exon1 (5UTR)
Flanking Genome Sequence: ET977 - 3-prime edge
```
GGTAGGCAAT TCTGAATCGC GAGGTTTAGT TCTCCGGCTG AATCAGAGAA 
CGACGAGTCG GAAAAATTCG AAACCCTAGA ACTGAATAAA TGTCGACGTG 
GCAATTATTT CCCGATTCCA GCGGCGATGG TTTCCGGTGG GAAGTCGCTG 
GCCGGATTCT TCAGTCCGTC TCGGATTCTA CGCCAACCAA GGCGCTCGAA 
TCGACAGCTC CTCTACCTTC TATGGCCGAT CTCTTGCTCC AAGGTGATTC 
T
```

3 prime edge [+] show sequence

Chromosome: 4; Position: 10423 [Map]; Strand: w
- AT4G00020.2 - [+] show detail - BREAST CANCER 2 like 2A
  Distance: -59; Region: promoter
- AT4G00020.1 - [+] show detail - BREAST CANCER 2 like 2A
  Distance: 0; Region: exon1 (5UTR)

GACCGTTTTC AGCCCTAGGT GTGGTAGGCA ATTCTGAATC GCGAGGTTTA 
GTTCTCCGGC TGAATCAGAG AACGACGAGT CGGAAAAATT CGAAACCCTA 
GAACTGAATA AATGTCGACG TGGCAATTAT TTCCCGATTC CAGCGGCGAT 
GGTTTCCGGT GGGAAGTCGC TGGCCGGATT CTTCAGTCCG TCTCGGATTC 
TACGCCAACC AAGGCGCTCG AATCGACAGC TCCTCTACCT TCTATGGCCG 
ATCTCTTGCT CCAAGGTGAT TCTTCGTTTC TTCCTTTTTT TGTCGAATCG 
TGTTTGTCAT GTGACTCAAA GGAGATTGAA TTATTATTTG ATGCTTCAAG 
GATGCTCGAA GCTTATAGCA CGGGAAGAAG CGATGCCTGG GGAAATCCCA 
ATGTTCAGGA CTGGGCTAGG GAAGTCTGTA NTTCTTAAAA AGTCTTCGAT 
TGCTAAAGCC AAATCCATTT TAGCAGAGAA GGTTACTTAC TCAGGTACTT 
TATGTGACAG TATTGAATTC CCTTGACTAG TCATTTGTTT CTGAATTCCC 
TTGAATTGTT ACTTAATGTT GGTGAAAATG GTTTCCTGAA AATCTTT

5 prime edge [+] show sequence

Chromosome: 4; Position: 10526 [Map]; Strand: w
- AT4G00020.2 - [+] show detail - BREAST CANCER 2 like 2A
  Distance: -162; Region: promoter
- AT4G00020.1 - [+] show detail - BREAST CANCER 2 like 2A
  Distance: -71; Region: promoter

TCTATTCNTN TCANGATGCG GAAATTTTAG NTCTATATTT TCTGTTTTCT 
TTCCCCCCAA AACCATCACC AAAAATCTAA AAAGTTAAAA GACGGCGGGA 
AAGTTGAAAA CGATTTGGGC CGAAATTAAC TTTGGGCTTG GTTTTAACGA 
CATAACGTCT CAAGACAAGT GAGTTAATGC CACGGCCTTT TATTGAGGTA 
AAAAAGGCAC GAATGCTAAC CGAAAATCGT TCCAAACAAC AATTGCTTAC 
GTGGACATTT TAAAACATCT GTTCAATGGT CATTGGTGAC TGACTTCAAT 
GAAAATTACA TTGCAACAAT AGCCAAGTAT CACAGAAAGT TACATTGCAA 
CAATTTCCAC GTCAAATTTC TAAACATGCA GAACGGTAAT TTACACGGGG 
NAAGGCGCTG AGGAGGCGGA GGGTGCTACG GAGGGGATCG CAGGAACACC 
GATGAATGAG AAGAATCCAG AGGCGGCATG ATCTTGCTCG TCGAGGANAG 
TGGATGATGC TCTATAAACG GCCAACAATA TGGTCGACGT ACACCTAAAA 
TGTCTTGCTA CGTGGATGCA CCACTAAACC TTTCAACTTT TTTGTGTTTT 
CTTTTCTTAA TTAAACACAA GATTAATTAG GAAATTTTGT GTTTCGTTCC 
GTTTCNTTTC NATTTCTGGT TTCTTGTTTC TATTACTCAA ATTATTGCTC 
CAATNTTAAG TTTGCTTGTA TCTACCCTTT GTTTTTTCCG TTNA

AT4G00020.1

Model type: Protein coding
Short Description: BREAST CANCER 2 like 2A
Curator Summary: Ortholog of breast cancer susceptibility protein 2. Essential at meiosis. Interacts with either AtRad51 or AtDmc1 and ATDSS1(I). Involved in embryo sac development.
Computational Description: BREAST CANCER 2 like 2A (BRCA2(IV)); FUNCTIONS IN: single-stranded DNA binding; INVOLVED IN: meiosis, embryo sac morphogenesis, embryo sac development, embryo development ending in seed dormancy; LOCATED IN: nucleus; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), DNA recombination/repair protein BRCA2, helical domain (InterPro:IPR015252), DNA recombination and repair protein, BRCA2 (InterPro:IPR011370), BRCA2, oligonucleotide/oligosaccharide-binding 1 (InterPro:IPR015187), Breast cancer type 2 susceptibility protein (InterPro:IPR015525), BRCA2 repeat (InterPro:IPR002093); BEST Arabidopsis thaliana protein match is: BRCA2-like B (TAIR:AT5G01630.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00020.2

Model type: Protein coding
Short Description: BREAST CANCER 2 like 2A
Curator Summary: Ortholog of breast cancer susceptibility protein 2. Essential at meiosis. Interacts with either AtRad51 or AtDmc1 and ATDSS1(I). Involved in embryo sac development.
Computational Description: BREAST CANCER 2 like 2A (BRCA2(IV)); FUNCTIONS IN: single-stranded DNA binding; INVOLVED IN: meiosis, embryo sac morphogenesis, embryo sac development, embryo development ending in seed dormancy; LOCATED IN: nucleus; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), DNA recombination/repair protein BRCA2, helical domain (InterPro:IPR015252), DNA recombination and repair protein, BRCA2 (InterPro:IPR011370), Breast cancer type 2 susceptibility protein (InterPro:IPR015525), BRCA2, oligonucleotide/oligosaccharide-binding 1 (InterPro:IPR015187), BRCA2 repeat (InterPro:IPR002093); BEST Arabidopsis thaliana protein match is: BRCA2-like B (TAIR:AT5G01630.1); Has 319 Blast hits to 211 proteins in 92 species: Archae - 0; Bacteria - 11; Metazoa - 140; Fungi - 22; Plants - 61; Viruses - 0; Other Eukaryotes - 85 (source: NCBI BLink).
Link: InterPro Scan - TAIR