F05848

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small) present in fewer numbers in organism (PATO:0001997) (Cauline leaf::Number::few)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) dark green (PATO:0001249) (Rosette leaf::Color::dark)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F05848

Determination of introduced cDNA(s)

RAFL07-07-M15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL07-07-M15 - Resource: pda03942

full reading [+] show sequence

GAGCTAACTA CCCAACCTGT GAAGCTACCC CCATTCTCTC TCAACGTTTT 
CGTTTTGAAA TGGCGAGGAA GAAGATCAGA GAGTATGACT CAAAGAGGTT 
GGTGAAGGAA CATTTCAAAA GGCTTTCTGG CAAAGAGCTT CCTATCAGAT 
CCGTTCAGAT TAATGAAACA ACTGATCTAA ATGAGCTAGT TGAAAAGGAA 
CCTTGGCTCT CGTCTGAGAA GCTGGTGGTG AAACCTGACA TGTTGTTTGG 
AAAGCGTGGC AAGAGTGGTT TGGTTGCCTT GAAATTAGAT TTTGCTGATG 
TTGCCACTTT TGTTAAAGAA CGTTTGGGAA AAGAGGTAGA GATGAGTGGA 
TGCAAAGGAC CCATAACAAC ATTCATAGTT GAACCATTTG TTCCACACAA 
TGAGGAGTAT TATCTCAATG TTGTCTCGGA TCGGCTTGGT TGCAGCATAA 
GCTTTTCTGA GTGTGGAGGA ATTGAGATCG AGGAGAACTG GGACAAGGTC 
AAGACAATAT TTTTACCAAC AGGTGCTTCC CTGACACCTG AAATATGTGC 
ACCTCTTGTC GCAACTCTTC CCTTAGAGAT CAAAGCTGAA ATTGAAGAAT 
TTATCAAAGT CATTTTCACC CTATTCCAAG ATCTTGATTT CACTTTCTTG 
GAGATGAATC CTTTCACTCT AGTTGATGGA AGTCCTTATC CTCTGGATAT 
GAGGGGTGAG CTTGATGATA CTGCTGCCTT CAAAAACTTT AAAAAATGGG 
GCGACATTGA ATTTCCTCTG CCATTTGGAA GAGTAATGAG TCCTACAGAA 
AGCTTTATCC ACGGACTGGA TGAGAAGACA AGTGCGTCTT TGAAGTTTAC 
CGTTCTGAAC CCCAAGGGAC GGATTTGGAC AATGGTAGCT GGTGGAGGAG 
CAAGTGTCAT CTATGCGGAT ACGGTTGGAG ATCTCGGGTA TGCATCTGAA 
CTTGGCAACT ATGCTGAATA CAGTGGAGCA CCCAAAGAAG ATGAGGTTTT 
GCAGTACGCC AGAGTCGTTA TTGATTGTGC TACAGCAAAC CCGGATGGAA 
AAAGCAGAGC CCTTGTCATC GGAGGCGGAA TTGCCAACTT CACTGACGTT 
GCTGCTACTT TCAATGGCAT AATCCGCGCT CTTAAAGAAA AGGAAGCAAA 
GCTGAAAGCA GCAAGGATGC ATATATTTGT GAGGAGAGGA GGACCAAACT 
ACCAAAAGGG ACTTGCTAAA ATGCGAGCCC TTGGAGATGA TATCGGTGTC 
CCCATCGAGG TCTATGGCCC AGAAGCAACC ATGACAGGTA TCTGCAAGGA 
GGCAATCCAG TACATCACAG CAGCAGCATA AGCTTCTCTC AGTACCTTCT 
ATGACCAAAA CTTTGTCTGT GTTTTAGAGC CTTTATTTAC TGTGGTTAAG 
ATTACTCAAG CTATAAGATA CTTGCAATTT CTTGAAAACT TCTGTTGTTC 
GATTCTCTTT CCCCTAACGT TTTCTTCAGA TTCAATAAAT AATCGTTACT 
TCTAAAAAAA AAAAAAAAA

Gene models with high sequence identity

AT1G10670.1 [+] show detail - ATP-citrate lyase A-1
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	ATP-grasp fold, succinyl-CoA synthetase-type	9.5e-17
superfamily	Glutathione synthetase ATP-binding domain-lik	5e-37
superfamily	Succinyl-CoA synthetase-like	2.6e-18
HMMPanther	ATP-CITRATE SYNTHASE	1.1e-166
Gene3D	ATP-grasp fold, subdomain 2	4.2e-17

See the detailed result >

AT1G10670.1

Model type: Protein coding
Short Description: ATP-citrate lyase A-1
Curator Summary: One of the three genes encoding subunit A of the trimeric protein ATP Citrate Lyase. Antisense ACLA-1 plants cause a reduction in cytosolic acetyl-CoA metabolism and have upregulation of stress-related genes and down-regulation of primary metabolism and growth genes, suggesting the mutation restricts normal growth and developmental processes and puts the plant into a state of stress.
Computational Description: ATP-citrate lyase A-1 (ACLA-1); CONTAINS InterPro DOMAIN/s: ATP-grasp fold, subdomain 2 (InterPro:IPR013816), ATP-grasp fold, succinyl-CoA synthetase-type (InterPro:IPR013650), Succinyl-CoA synthetase-like (InterPro:IPR016102); BEST Arabidopsis thaliana protein match is: ATP-citrate lyase A-2 (TAIR:AT1G60810.1); Has 5465 Blast hits to 5464 proteins in 1555 species: Archae - 120; Bacteria - 3046; Metazoa - 362; Fungi - 200; Plants - 107; Viruses - 0; Other Eukaryotes - 1630 (source: NCBI BLink).
Link: InterPro Scan - TAIR