F06609

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F06609

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F06609

Determination of introduced cDNA(s)

  • RAFL06-78-M16 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-78-M16 - Resource: pda03748

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-78-M16 - full reading 
    CTGTACCGCA AAATCCTCGT CGAAGCTCGC TGCTGCAACC ATGTCCGACG 
    AGGAGCATCA CTTTGAGTCC AGTGACGCCG GAGCGTCCAA AACCTACCCT 
    CAACAAGCTG GAACCATCCG TAAGAATGGT TACATCGTCA TCAAAAATCG 
    TCCCTGCAAG GTTGTTGAGG TTTCAACCTC GAAGACTGGC AAGCATGGTC 
    ATGCTAAATG TCATTTTGTA GCTATTGATA TCTTCACCAG CAAGAAACTC 
    GAAGATATTG TTCCTTCTTC CCACAATTGT GATGTTCCTC ATGTCAACCG 
    TACTGATTAT CAGCTGATTG ACATTTCTGA AGATGGATAT GTCAGTTTGT 
    TGACTGATAA CGGTAGTACC AAGGATGACC TTAAGCTCCC TAATGATGAC 
    ACTCTGCTCC AACAGATCAA GAGTGGGTTT GATGATGGAA AAGATCTAGT 
    GGTGAGTGTG ATGTCAGCTA TGGGAGAGGA ACAGATCAAT GCTCTTAAGG 
    ACATCGGTCC CAAGTGAGAC TAACAAAGCC TCCCCTTTGT TATGAGATTC 
    TTCTTCTTCT TCTGTAGGCT TCCATTACTC ATCGGAGATT ATCTTGTTTT 
    TGGGTGACTC CTATTTTGGA TATTTAAACT TTTGTTAATA ATGCCATCTT 
    CTTCAACCTG AAAAAAAAAA AAAAAA

    Gene models with high sequence identity

    1. AT1G13950.1 [+] show detail - eukaryotic elongation factor 5A-1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Translation protein SH3-like, subgroup 3.1e-27
    Gene3D Nucleic acid-binding, OB-fold 9.5e-26
    HMMPIR Translation elongation factor, IF5A 2e-98
    HMMTigr Translation elongation factor, IF5A 3.1e-55
    HMMPanther INITIATION FACTOR 5A 2.4e-111
    HMMPanther Translation elongation factor, IF5A 2.4e-111
    PatternScan Translation elongation factor, IF5A, hypusine site 0
    superfamily Translation protein SH3-like 2.2e-28
    superfamily Nucleic acid-binding, OB-fold-like 1.2e-27
    HMMPfam Translation elongation factor, IF5A C-terminal 3.9e-30
    HMMPfam KOW 4.8e-05

    See the detailed result >

    AT1G13950.1

    Model type
    Protein coding
    Short Description
    eukaryotic elongation factor 5A-1
    Curator Summary
    Encodes eukaryotic translation initiation factor 5A (EIF-5A).In mammalian cells it functions as a shuttle protein that translocates mRNA from the nucleus to cytoplasmic ribosomes. Overexpression results in an increase in both primary and secondary xylem formation. In RNAi suppressed lines, xylem formation is reduced.
    Computational Description
    eukaryotic elongation factor 5A-1 (ELF5A-1); FUNCTIONS IN: ribosome binding, RNA binding, translation elongation factor activity, translation initiation factor activity; INVOLVED IN: translational initiation, xylem development; LOCATED IN: cellular_component unknown; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold (InterPro:IPR012340), Translation elongation factor, IF5A, hypusine site (InterPro:IPR019769), Translation protein SH3-like, subgroup (InterPro:IPR014722), Translation elongation factor, IF5A (InterPro:IPR001884), Translation elongation factor, IF5A C-terminal (InterPro:IPR020189), Translation protein SH3-like (InterPro:IPR008991), Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), KOW (InterPro:IPR005824); BEST Arabidopsis thaliana protein match is: eukaryotic elongation factor 5A-3 (TAIR:AT1G69410.1); Has 1356 Blast hits to 1355 proteins in 415 species: Archae - 264; Bacteria - 0; Metazoa - 366; Fungi - 246; Plants - 261; Viruses - 0; Other Eukaryotes - 219 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR