F07004

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F07004

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F07004

Determination of introduced cDNA(s)

  • RAFL09-97-K19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-97-K19 - Resource: pda10034

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-97-K19 - full reading
    AGATCGAATT TGATCAGTGA GAGAGGAGGA GATATGGGTG CTCAGAAGAA 
    AGGCGGCGCC GCCGCTAGGG TTTCAGAGGA TGCGGAGGTA CAGAGTCGCC
    ACCGATTGCA AGCAATTCTT CTTGCCGACA GTTTCGCCAC GAAATTTCGT
    CCCGTAACCC TAGAACGCCC AAAGGTACTG TTACCGATAG TTAATGTTCC
    GATGATTGAT TACACATTGG CATGGCTCGA ATCTGCTGGA ATAGAGGAGG
    TTTTCGTCTT CTGTTGTGCT CATTCGATGC AGGTTATCGA ATATCTGGAG
    AAATCCGAGT GGTATTCGCA TCCAAACTTG CTTGTGAGAA CGATTGAGTC
    GCACAAATCC ATCAGTGCTG GAGATGCTTT GCGTTACATG TATGAACAAC
    AGACAGAGAC GTCTCAGATT CAAGGTGATT TCGTTCTTGT TAGTGGAGAT
    ACTGTGAGTA ATATGCCACT TGCTGATTTG ATTCAAGAGC ATAGAGAGAG
    GAAGAAGAAA GATGAGAAAG CTATTATGAC TATGGTTATT AAGCAGTCTA
    AATCTTCTCC ACTTACGCAT CAGTCAAGAT TAGGAACAGA TCAGCTTTTC
    ATTGCTGTTG ATCCTTTGAC AAAGCAGCTT CTTCATTACG AGGAAGATAA
    GATTGATCAT CCAAGTGGGT CAGTTTGCTT AGAGAAGTCA TTGTTGGATA
    CTAATCCTTC TGTTTTAGTA TGCAATGATA TGCAGGATTG CTACATTGAC
    ATATGTTCTC CTGAAGTGCT TAGTCTCTTT GAGGATAACT TTGATTATCA
    ACATCTGCGT CGTCATTTTG TCAAAGGTGT GCTTGTGGAT GATATTATGG
    GTTATAAAAT CTTCACTCAT GAGATTCACT CGAGCTATGC TGGTAGGATT
    GATAATTTCC GAAGCTATGA CACTGTTAGC AAGGATATAA TTCAGAGGTG
    GACATATCCT TATGTACCAG ATATTAATTT CAGTGGAAAT CGGCCTCTAA
    AGCTCGGGAG ACAAGGGATA TACAAGGCTT CTGATGTAGT TCAATCACGA
    TCTGCTGATG TTGGAGCTTC TACTGTCATT GGATACGGTA CGAAAATTGG
    TCATGGGGAT AAAATCATGA ACTCGGTTAT TGGGAATGGA TGCTCTATTG
    GATCAAATGT GGTGATTGAA GGCTCATACA TATGGAACAA TGTTACTATT
    GAAGATGGGT GTGAGATAAG GAACGCTATT GTGTGTGATG GGGTGAAAAT
    CAGAGCAGGA GCTGTTCTGC AGCCTGGTGT TGTTTTGTCT TTCAATGTTG
    TAGTTGGACG GGACTTTGTT GTGCCTGCAT ACTCAAAGGT TTCCCTACTT
    CAACAGCCAA CGACAGAAGA CAGTGATGAA GAGCTGGAAT ATGCTGATAG
    TAGCAGCGGG ACTGCGGATC ATTTGTCAGG CCTAAATCTG CAAATGGAGT
    CCAAAGCATC TGAGCTTGGT CCTGATGGAG CAGGTTACAT ATGGGAAGTA
    TGTGAAGGGG CTCATGATGA GGAGTGGAAG CATTCTGTTG CACCAATCCC
    CAAGGATAAA CTTTCTGAGA TTACTCAGGC CATAGACGAT GATGACACAG
    ATGATGAAAG TGTCGTCCCG ACTTCTGGAG AGTTGAAATC TGATGCTGAT
    AGTATTAACA CTGATGTGAA TGATCCCAAT GATGATTACT ATTACTTTGA
    GAAAGAGGTT GAAGGGACCG TCTTAAGGGC TGTTGAAGAG AATATCAAAG
    TGGACCTTGT AACTATGGAG ATTAACGGGC TGCGGTTGTC ATTCAACATG
    GAATCTGCAG ATTGTGCTGG AGCAACATTT TTCTCGATGA TAAAACTGGC
    ACTTGATACT CCACACAATT CTGGTAGTGA GTTGTATAAA AACGCAGCGA
    GTATCATTAC AAAATGGAAG GATCTTCTTG GCTTCTACGC AAAGAAAATT
    GATGAACAGA TAGAAGTGAT CATGAAATTT GAAGAGATGT GTCAGGAATC
    TCACAAAGAG TTGGGCCCTT TGTTCACACA AATTCTACAT CTTCTATATG
    ACAAGGACGT GTTGCAAGAA GATGCGATAC TGAGATGGGA GGAAGAGAAG
    GCAGGCGCGG ATGAAGCTGA CAAAGTATAT CTGAAACAAT GTGATACATT
    CATACAGTGG CTCAAAGAAG CATCTGAGGA GGAAGATGAA GATGATGAAG
    ATGAAGAAGA AGAAGAAGAT AACTGAATTG GTATACCTGA GTTCGATGGC
    TCTCAACTAG TTGTTCATTA TGCAGTTGAT GGCCTCACAT TTTGATTGAT
    CATTTACTTT TAAGAGCTAC TCTGTTTTTT GTTCTGTCAT CATCAATAAT
    CTTTTTTTCT TCATGTCTTC TGTTCTGTTG CTTCATGAGA TACTTTCTCA
    TCCACGAGTT TGCTCACATG TATCGTTTGA TATATAATTT TACTTGAGTA
    CTAAATCTCT TAATTTTTAT TTGC

    Gene models with high sequence identity

    1. AT2G34970.1 [+] show detail - Trimeric LpxA-like enzyme
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan eIF4-gamma/eIF5/eIF2-epsilon 27.131
    superfamily Armadillo-type fold 2.8e-44
    superfamily Nucleotide-diphospho-sugar transferases 7e-40
    superfamily Trimeric LpxA-like 3.2e-25
    Gene3D no description 1.4e-23
    Gene3D no description 1.7e-10
    Gene3D eIF4-gamma/eIF5/eIF2-epsilon 4.4e-49
    HMMSmart eIF4-gamma/eIF5/eIF2-epsilon 6.7e-36
    Coil coiled-coil 0
    HMMPanther EUKARIOTIC TRANSLATION INITIATION FACTOR 2B, 1.5e-213
    HMMPanther SUGAR-1-PHOSPHATE GUANYL TRANSFERASE 1.5e-213
    HMMPfam eIF4-gamma/eIF5/eIF2-epsilon 8.8e-20
    HMMPfam Nucleotidyl transferase 1.6e-11

    See the detailed result >

    AT2G34970.1

    Model type
    Protein coding
    Short Description
    Trimeric LpxA-like enzyme
    Computational Description
    Trimeric LpxA-like enzyme; FUNCTIONS IN: binding, transferase activity, translation initiation factor activity, nucleotidyltransferase activity; INVOLVED IN: biosynthetic process, regulation of translational initiation; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Trimeric LpxA-like (InterPro:IPR011004), eIF4-gamma/eIF5/eIF2-epsilon (InterPro:IPR003307), Armadillo-type fold (InterPro:IPR016024), Nucleotidyl transferase (InterPro:IPR005835); BEST Arabidopsis thaliana protein match is: Trimeric LpxA-like enzyme (TAIR:AT4G18300.1); Has 8121 Blast hits to 7961 proteins in 1548 species: Archae - 574; Bacteria - 3735; Metazoa - 1296; Fungi - 469; Plants - 387; Viruses - 0; Other Eukaryotes - 1660 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR