F07004

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	cauline leaf (PO:0000013)	decreased width (PATO:0000599) (Cauline leaf::Shape::narrow) abnormal (PATO:0000460) (Cauline leaf::Others)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) abnormal (PATO:0000460) (Rosette leaf::Others)
	fruit (PO:0009001)	decreased length (PATO:0000574) (Silique::Shape::short)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others) abnormal (PATO:0000460) (Stem::Others)
T2
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	desaturated green (PATO:0001272) (Rosette leaf::Color::pale)
	fruit (PO:0009001)	abnormal (PATO:0000460) (Silique::Others)
	stem (PO:0009047)	desaturated green (PATO:0001272) (Stem::Color::pale)
	stem internode (PO:0020142)	decreased length (PATO:0000574) (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F07004

Determination of introduced cDNA(s)

RAFL09-97-K19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-97-K19 - Resource: pda10034

full reading [+] show sequence

AGATCGAATT TGATCAGTGA GAGAGGAGGA GATATGGGTG CTCAGAAGAA 
AGGCGGCGCC GCCGCTAGGG TTTCAGAGGA TGCGGAGGTA CAGAGTCGCC 
ACCGATTGCA AGCAATTCTT CTTGCCGACA GTTTCGCCAC GAAATTTCGT 
CCCGTAACCC TAGAACGCCC AAAGGTACTG TTACCGATAG TTAATGTTCC 
GATGATTGAT TACACATTGG CATGGCTCGA ATCTGCTGGA ATAGAGGAGG 
TTTTCGTCTT CTGTTGTGCT CATTCGATGC AGGTTATCGA ATATCTGGAG 
AAATCCGAGT GGTATTCGCA TCCAAACTTG CTTGTGAGAA CGATTGAGTC 
GCACAAATCC ATCAGTGCTG GAGATGCTTT GCGTTACATG TATGAACAAC 
AGACAGAGAC GTCTCAGATT CAAGGTGATT TCGTTCTTGT TAGTGGAGAT 
ACTGTGAGTA ATATGCCACT TGCTGATTTG ATTCAAGAGC ATAGAGAGAG 
GAAGAAGAAA GATGAGAAAG CTATTATGAC TATGGTTATT AAGCAGTCTA 
AATCTTCTCC ACTTACGCAT CAGTCAAGAT TAGGAACAGA TCAGCTTTTC 
ATTGCTGTTG ATCCTTTGAC AAAGCAGCTT CTTCATTACG AGGAAGATAA 
GATTGATCAT CCAAGTGGGT CAGTTTGCTT AGAGAAGTCA TTGTTGGATA 
CTAATCCTTC TGTTTTAGTA TGCAATGATA TGCAGGATTG CTACATTGAC 
ATATGTTCTC CTGAAGTGCT TAGTCTCTTT GAGGATAACT TTGATTATCA 
ACATCTGCGT CGTCATTTTG TCAAAGGTGT GCTTGTGGAT GATATTATGG 
GTTATAAAAT CTTCACTCAT GAGATTCACT CGAGCTATGC TGGTAGGATT 
GATAATTTCC GAAGCTATGA CACTGTTAGC AAGGATATAA TTCAGAGGTG 
GACATATCCT TATGTACCAG ATATTAATTT CAGTGGAAAT CGGCCTCTAA 
AGCTCGGGAG ACAAGGGATA TACAAGGCTT CTGATGTAGT TCAATCACGA 
TCTGCTGATG TTGGAGCTTC TACTGTCATT GGATACGGTA CGAAAATTGG 
TCATGGGGAT AAAATCATGA ACTCGGTTAT TGGGAATGGA TGCTCTATTG 
GATCAAATGT GGTGATTGAA GGCTCATACA TATGGAACAA TGTTACTATT 
GAAGATGGGT GTGAGATAAG GAACGCTATT GTGTGTGATG GGGTGAAAAT 
CAGAGCAGGA GCTGTTCTGC AGCCTGGTGT TGTTTTGTCT TTCAATGTTG 
TAGTTGGACG GGACTTTGTT GTGCCTGCAT ACTCAAAGGT TTCCCTACTT 
CAACAGCCAA CGACAGAAGA CAGTGATGAA GAGCTGGAAT ATGCTGATAG 
TAGCAGCGGG ACTGCGGATC ATTTGTCAGG CCTAAATCTG CAAATGGAGT 
CCAAAGCATC TGAGCTTGGT CCTGATGGAG CAGGTTACAT ATGGGAAGTA 
TGTGAAGGGG CTCATGATGA GGAGTGGAAG CATTCTGTTG CACCAATCCC 
CAAGGATAAA CTTTCTGAGA TTACTCAGGC CATAGACGAT GATGACACAG 
ATGATGAAAG TGTCGTCCCG ACTTCTGGAG AGTTGAAATC TGATGCTGAT 
AGTATTAACA CTGATGTGAA TGATCCCAAT GATGATTACT ATTACTTTGA 
GAAAGAGGTT GAAGGGACCG TCTTAAGGGC TGTTGAAGAG AATATCAAAG 
TGGACCTTGT AACTATGGAG ATTAACGGGC TGCGGTTGTC ATTCAACATG 
GAATCTGCAG ATTGTGCTGG AGCAACATTT TTCTCGATGA TAAAACTGGC 
ACTTGATACT CCACACAATT CTGGTAGTGA GTTGTATAAA AACGCAGCGA 
GTATCATTAC AAAATGGAAG GATCTTCTTG GCTTCTACGC AAAGAAAATT 
GATGAACAGA TAGAAGTGAT CATGAAATTT GAAGAGATGT GTCAGGAATC 
TCACAAAGAG TTGGGCCCTT TGTTCACACA AATTCTACAT CTTCTATATG 
ACAAGGACGT GTTGCAAGAA GATGCGATAC TGAGATGGGA GGAAGAGAAG 
GCAGGCGCGG ATGAAGCTGA CAAAGTATAT CTGAAACAAT GTGATACATT 
CATACAGTGG CTCAAAGAAG CATCTGAGGA GGAAGATGAA GATGATGAAG 
ATGAAGAAGA AGAAGAAGAT AACTGAATTG GTATACCTGA GTTCGATGGC 
TCTCAACTAG TTGTTCATTA TGCAGTTGAT GGCCTCACAT TTTGATTGAT 
CATTTACTTT TAAGAGCTAC TCTGTTTTTT GTTCTGTCAT CATCAATAAT 
CTTTTTTTCT TCATGTCTTC TGTTCTGTTG CTTCATGAGA TACTTTCTCA 
TCCACGAGTT TGCTCACATG TATCGTTTGA TATATAATTT TACTTGAGTA 
CTAAATCTCT TAATTTTTAT TTGC

Gene models with high sequence identity

AT2G34970.1 [+] show detail - Trimeric LpxA-like enzyme
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
ProfileScan	eIF4-gamma/eIF5/eIF2-epsilon	27.131
superfamily	Armadillo-type fold	2.8e-44
superfamily	Nucleotide-diphospho-sugar transferases	7e-40
superfamily	Trimeric LpxA-like	3.2e-25
Gene3D	no description	1.4e-23
Gene3D	no description	1.7e-10
Gene3D	eIF4-gamma/eIF5/eIF2-epsilon	4.4e-49
HMMSmart	eIF4-gamma/eIF5/eIF2-epsilon	6.7e-36
Coil	coiled-coil	0
HMMPanther	EUKARIOTIC TRANSLATION INITIATION FACTOR 2B,	1.5e-213
HMMPanther	SUGAR-1-PHOSPHATE GUANYL TRANSFERASE	1.5e-213
HMMPfam	eIF4-gamma/eIF5/eIF2-epsilon	8.8e-20
HMMPfam	Nucleotidyl transferase	1.6e-11

See the detailed result >

AT2G34970.1

Model type: Protein coding
Short Description: Trimeric LpxA-like enzyme
Computational Description: Trimeric LpxA-like enzyme; FUNCTIONS IN: binding, transferase activity, translation initiation factor activity, nucleotidyltransferase activity; INVOLVED IN: biosynthetic process, regulation of translational initiation; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Trimeric LpxA-like (InterPro:IPR011004), eIF4-gamma/eIF5/eIF2-epsilon (InterPro:IPR003307), Armadillo-type fold (InterPro:IPR016024), Nucleotidyl transferase (InterPro:IPR005835); BEST Arabidopsis thaliana protein match is: Trimeric LpxA-like enzyme (TAIR:AT4G18300.1); Has 8121 Blast hits to 7961 proteins in 1548 species: Archae - 574; Bacteria - 3735; Metazoa - 1296; Fungi - 469; Plants - 387; Viruses - 0; Other Eukaryotes - 1660 (source: NCBI BLink).
Link: InterPro Scan - TAIR