F08310

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F08310

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F08310

Determination of introduced cDNA(s)

  • RAFL14-56-E23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL14-56-E23 - Resource: pda18782

    full reading [+] show sequence

    Nucleotide sequence: RAFL14-56-E23 - full reading
    GTGAATTCAC AGTTGAGCGA GATGAAAAGT ACGGTGGTAA CAGGACCTTC 
    AAAAGCTTTG AAGACATCAC CACTGACTAT GAAAGCGGCC AGTTGCACCC
    AAAGGATTTG AAGGACGCTC TGTCAAAAGC GTTGAATAAG ATACTGCAAC
    CTGTTCGTGA TCATTTCAAA CCAACAGCCG CGCCAAGAAT CTGCTCAAAC
    AGGTCAAGGG TTACAAAGTC ACCAGAGTCA TACCAACAGC TTCATCTACA
    AAGGAGGAGG ATCTTTCCAT CAACACATCA GCATCTTCTT CGGCCGCCGG
    ATTACAGATG AGCGAGGAGG CCGAGATGAA ATACAAGATT GTGAGGAGTA
    TTGGTGAGGA ATGCATACAA GAGGATGAGC TGAAGAATCT TCTAGCCAAG
    AAGCCTGCTC CTATCTGCTA CGATGGTTTT GAACCATCAG GAAGGATGCA
    CATCGCCCAG GGAGTTATGA AGGTCACCAA TGTGAACAAA TTGACTTCAG
    CTGGTTGCCA AGTGAAAATT TGGATAGCAG ATTGGTTTGC TCAATTGAAC
    AACAAGTTGG GAGGTGACTT GGAGAGAATT AGAGTGGTAG GAGAATATTT
    CAAGGAAATT TGGCAAGCAG GTGGGATGAA CAATGACAAA GTAGAGTTTC
    TTTGGGCATC AGACGAAATA AATGGCAAGG GGAGTAAGTA TTGGCCTCTT
    GTGATGGACA TTGCTCGTAG AAACAACCTA CGTCGAATCT TGAGGTGTGG
    GCAGATTATG GGACGTAGTG AGACTGAGGT GCTGAGTGCA GCTCAAATTC
    TTTACCCCTG TATGCAGTGT GCGGATATTT TCTTACTTGA GGCTGATATT
    TGCCAGCTCG GGATGGATCA GCGAAAAGTA AACATGCTAG CAAGAGAATA
    CTGTGCTGAC ATTAAGAGGA AAAATAAACC CATCATCTTG TCCCACCATA
    TGCTTCCTGG TCTCCAACAA GGACAAGAAA AGATGTCCAA AAGTGATCCA
    TCATCTGCAA TATTTATGGA AGATGAGGAG GCTGATGTTA ATGAGAAGAT
    CAGCAAAGCC TATTGCCCTC CGAAAACTGT GGAAGGCAAT CCATGTCTCG
    AGTATGTCAA ATACATTGTT CTACCACGAT TCAATGAGTT CAAGGTTGAA
    AGTGAAAAGA ATGGCGGCAA TAAGACCTTC AACAGCTTTG AAGATATTGT
    CGCCGACTAC GAGACCGGCG AGCTACATCC CGAGGATCTG AAGAAAGCTC
    TGATGAAAGC ATTGAATATA ACTCTGCAAC CTGTTCGCGA TCACTTTAAA
    ACCAACGAGC GTGCCAAAAA TCTACTGGAA CAGGTCAAGG CTTTTAGAGT
    CACCAGGTGA AGTTGCAGAA GCAAAGAGAG TATTTGTGGA CATCAGTTAA
    AGAGCTTTCC TAAGGATTTT GGTGGTTACT AAGAAAGCTA ACAAATCTCT
    TAAAGTTCAT TTTTGTTTTT TGATAGATAT GAGCCTCGAA GATTAGAATC
    CAGTTGGACA AATTTTGTGA TTTATTGTCA ACTAAAAATA AACCTCCACA
    GCTACAAGAG CATTAAACTA AAATTTTCCA GTAGAAAAA

    Gene models with high sequence identity

    1. AT1G28350.1 [+] show detail - Nucleotidylyl transferase superfamily protein
      E-value: 0; Score: 99.87

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Aminoacyl-tRNA synthetase, class Ib 2e-65
    HMMPfam Aminoacyl-tRNA synthetase, class Ib 9.5e-05
    HMMPIR Tyrosine tRNA ligase, archaeal/eukaryotic 8.7e-203
    superfamily Nucleotidylyl transferase 2.7e-85
    superfamily Nucleotidylyl transferase 5.5e-10
    HMMPanther Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic 3.5e-162
    HMMPanther ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHION 3.5e-162
    Gene3D Rossmann-like alpha/beta/alpha sandwich fold 5.6e-68
    Gene3D no description 6.8e-39
    Gene3D no description 3e-13

    See the detailed result >

    AT1G28350.1

    Model type
    Protein coding
    Short Description
    Nucleotidylyl transferase superfamily protein
    Computational Description
    Nucleotidylyl transferase superfamily protein; FUNCTIONS IN: tyrosine-tRNA ligase activity, nucleotide binding, aminoacyl-tRNA ligase activity, ATP binding; INVOLVED IN: translation, tyrosyl-tRNA aminoacylation, tRNA aminoacylation for protein translation; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic (InterPro:IPR015624), Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial (InterPro:IPR002307), Aminoacyl-tRNA synthetase, class Ib (InterPro:IPR002305); BEST Arabidopsis thaliana protein match is: Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial (TAIR:AT2G33840.1); Has 6761 Blast hits to 4745 proteins in 1539 species: Archae - 718; Bacteria - 3084; Metazoa - 577; Fungi - 521; Plants - 241; Viruses - 10; Other Eukaryotes - 1610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR