F09228

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F09228

Determination of introduced cDNA(s)

RAFL02-03-L07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL02-03-L07 - Resource: pda00132

full reading [+] show sequence

GCCAACACAA CTCCTTATAA GATATACACT TCATCAACCT ATAGATCTCA 
CTCACATAAT CAACCTACAA AACAAAAACA AGAATGGGTA GATCCAACGA 
GCAAGATCTG CTCTCTACCG AGATCGTTAA TCGTGGGATC GAACCATCCG 
GTCCTAACGC CGGCTCACCA ACGTTCTCGG TTAGGGTCAG GAGACGTTTG 
CCTGATTTTC TTCAGTCGGT GAACTTGAAG TACGTGAAAC TTGGTTACCA 
CTACCTCATA AACCATGCGG TTTATTTGGC GACCATACCG GTTCTTGTGC 
TGGTTTTTAG TGCTGAGGTT GGGAGTTTAA GCAGAGAAGA GATTTGGAAG 
AAGCTTTGGG ACTATGATCT TGCAACTGTT ATCGGATTCT TCGGTGTCTT 
TGTTTTAACC GCTTGTGTCT ACTTCATGTC TCGTCCTCGC TCTGTTTATC 
TTATTGATTT CGCTTGTTAC AAGCCCTCCG ATGAACACAA GGTGACAAAA 
GAAGAGTTCA TAGAACTAGC GAGAAAATCA GGGAAGTTCG ACGAAGAGAC 
ACTCGGTTTC AAGAAGAGGA TCTTACAAGC CTCAGGCATA GGCGACGAGA 
CATACGTCCC AAGATCCATC TCTTCATCAG AAAACATAAC AACGATGAAA 
GAAGGTCGTG AAGAAGCCTC TACAGTGATC TTTGGAGCAC TAGACGAACT 
CTTCGAGAAG ACACGTGTAA AACCTAAAGA CGTTGGTGTC CTTGTGGTTA 
ACTGTAGCAT TTTCAACCCG ACACCGTCGT TGTCCGCAAT GGTGATAAAC 
CATTACAAGA TGAGAGGGAA CATACTTAGT TACAACCTTG GAGGGATGGG 
ATGTTCGGCT GGAATCATAG CTATTGATCT TGCTCGTGAC ATGCTTCAGT 
CTAACCCTAA TAGTTATGCT GTTGTTGTGA GTACTGAGAT GGTTGGGTAT 
AATTGGTACG TGGGAAGTGA CAAGTCAATG GTTATACCTA ATTGTTTCTT 
TAGGATGGGT TGTTCTGCCG TTATGCTCTC TAACCGTCGT CGTGACTTTC 
GCCATGCTAA GTACCGTCTC GAGCACATTG TCCGAACTCA TAAGGCTGCT 
GACGACCGTA GCTTCAGGAG TGTGTACCAG GAAGAAGATG AACAAGGATT 
CAAGGGGTTG AAGATAAGTA GAGACTTAAT GGAAGTTGGA GGTGAAGCTC 
TCAAGACAAA CATCACTACC TTAGGTCCTC TTGTCCTACC TTTCTCCGAG 
CAGCTTCTCT TCTTTGCTGC TTTGCTCCGC CGAACATTCT CACCTGCTGC 
CAAAACGTCC ACAACCACTT CCTTCTCTAC TTCCGCCACC GCAAAAACCA 
ATGGAATCAA GTCTTCCTCT TCCGATCTGT CCAAGCCATA CATCCCGGAC 
TACAAGCTCG CCTTCGAGCA TTTTTGCTTC CACGCGGCAA GCAAAGTAGT 
GCTTGAAGAG CTTCAAAAGA ATCTAGGCTT GAGTGAAGAG AATATGGAGG 
CTTCTAGGAT GACACTTCAC AGGTTTGGAA ACACTTCTAG CAGTGGAATC 
TGGTATGAGT TGGCTTACAT GGAGGCCAAG GAAAGTGTTC GTAGAGGCGA 
TAGGGTTTGG CAGATCGCTT TCGGTTCTGG TTTTAAGTGT AACAGTGTGG 
TGTGGAAGGC AATGAGGAAG GTGAAGAAGC CAACCAGGAA CAATCCTTGG 
GTGGATTGCA TCAACCGTTA CCCTGTGCCT CTCTAAATTA TCATTCTTCT 
AAATTAAATC AAGTAAGATC TCTAATTACT CCAACCAAAA GATACAGTTT 
GGTTGGATGA TAGGAGTTAT TTACTGATCA TTCGTATCTA AGTCTGTTAT 
AAGAATGGAT GTGGCTAGAG TCCTGTTCAG CTTCAACTTG TTTTATTTTT 
TGTTTGTTCC C

Gene models with high sequence identity

AT2G26250.1 [+] show detail - 3-ketoacyl-CoA synthase 10
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	FAE1/Type III polyketide synthase-like protein	4.9e-137
HMMPfam	3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal	1.4e-12
Coil	coiled-coil	0
HMMPIR	Very-long-chain 3-ketoacyl-CoA synthase	8.2e-271
superfamily	Thiolase-like	3.9e-90
Gene3D	Thiolase-like, subgroup	7.8e-53
Gene3D	Thiolase-like, subgroup	2e-21

See the detailed result >

AT2G26250.1

Model type: Protein coding
Short Description: 3-ketoacyl-CoA synthase 10
Curator Summary: epidermis-specific, encodes KCS10, a putative 3-ketoacyl-CoA synthase. probably involved in the synthesis of long-chain lipids found in the cuticle.
Computational Description: 3-ketoacyl-CoA synthase 10 (KCS10); FUNCTIONS IN: transferase activity, transferring acyl groups other than amino-acyl groups, catalytic activity, acyltransferase activity; INVOLVED IN: in 8 processes; LOCATED IN: endoplasmic reticulum, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase-like (InterPro:IPR016039), Very-long-chain 3-ketoacyl-CoA synthase (InterPro:IPR012392), 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (InterPro:IPR013747), FAE1/Type III polyketide synthase-like protein (InterPro:IPR013601), Thiolase-like, subgroup (InterPro:IPR016038); BEST Arabidopsis thaliana protein match is: 3-ketoacyl-CoA synthase 4 (TAIR:AT1G19440.1); Has 1723 Blast hits to 1687 proteins in 351 species: Archae - 0; Bacteria - 632; Metazoa - 0; Fungi - 0; Plants - 985; Viruses - 0; Other Eukaryotes - 106 (source: NCBI BLink).
Link: InterPro Scan - TAIR