F09732

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F09732

Determination of introduced cDNA(s)

RAFL08-16-P09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL08-16-P09 - Resource: pda04927

full reading [+] show sequence

GAGAGGACGA AGCTTGACAC TCTCAAGAGT AAAGCTCGAG CTTGCGAGAA 
GAAGCATGTC TGCAACATCC GTACCTTCAA TGGCGGATTT TCTCACCAAA 
AAACCTTACT CTCCTCCTTC TTGGGCTTCT CATCTTCGTC CGCTTCCTTC 
TCACACTTTC TCCCTCGCTC ACCTTCCTAC TCCGATCCAT CGATGGAATC 
TTCCTGGTCT TCCTAATGGC ACAGAACTCT GGATCAAGCG AGATGATTTC 
ACCGGAATGG AATTGAGTGG AAACAAAGTA CGAAAACTCG AATTCTTAAT 
GGCGGAAGCT GTTGATCAAC ACGCTGATAC TGTAATCACT ATCGGCGGTA 
TTCAGAGCAA TCATTGTCGT GCTACAGCCA CTGCATCTAA CTATCTTAAT 
CTCAATTCTC ATCTTATTCT CCGTACTTCC AAGCTTCTTG CTGATGAAGA 
TCCTGGATTG GTTGGGAATC TCCTTGTCGA GCGTCTCGTT GGAGCTAATG 
TTCATCTAAT CTCTAAAGAA GAGTATTCTT CCATTGGGAG TGAGGCTCTT 
ACTAATGCTC TGAAAGAGAA ACTGGAAAAA GAAGGAAAGA AACCCTATGT 
TATTCCAGTC GGTGGATCGA ACTCTTTGGG AACTTGGGGT TATATAGAAG 
CAGCAAGGGA AATTGAGGAG CAGCTGAATT ATAGACCCGA TGACCTGAAA 
TTTGATGATA TTGTGGTAGC ATGTGGCAGT GGTGGTACAA TTGCTGGTAT 
TTCATTGGGG TCTTGGTTGG GAGCTCTAAA AGCCAAGGTT CATGCTTTCT 
CGGTTTGCGA TGATCCTGAT TACTTCTATG ACTTTGTCCA AGGGCTTCTG 
GATGGACTTC ACGCTGGTGT TAACTCTCGT GATATCGTCA ACATCCACAA 
TGCCAAAGGA AAAGGATATG CCATGAACAC GTCAGAGGAG CTTGAGTTTG 
TAAAGAAAGT AGCAAGTTCA ACTGGTGTTA TTCTTGATCC GGTTTACAGT 
GGGAAAGCTG CGTATGGTTT GATAAATGAG ATCACCAAAG ATCCCAAATG 
TTGGGAGGGA AGGAAGATAT TGTTCATACA CACTGGTGGG CTTCTTGGGT 
TGTATGATAA GGTTGATCAA ATGGCATCTC TGATGGGTAA TTGGTCCCGG 
ATGGATGTTT CAGAATCCGT TCCAAGAAAA GATGGTGTTG GGAAAATGTT 
CTAGCACAAC AAAATCTGAT TTGTAATAAT CCTGTGAAAA TAAGACAGAT 
TGAACAATTT GTGCATTTGG CAGTGAATAA ACACATCTCT GTTTT

Gene models with high sequence identity

AT1G48420.1 [+] show detail - D-cysteine desulfhydrase
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
Gene3D	no description	1.2e-55
HMMPfam	Pyridoxal phosphate-dependent enzyme, beta subunit	8.5e-27
HMMTigr	Pyridoxal phosphate-dependent deaminase	2.8e-239
HMMPanther	1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE	1.5e-157
HMMPanther	SER/THR DEHYDRATASE, TRP SYNTHASE	1.5e-157
superfamily	Pyridoxal phosphate-dependent enzyme, beta subunit	8.5e-66

See the detailed result >

AT1G48420.1

Model type: Protein coding
Short Description: D-cysteine desulfhydrase
Curator Summary: Encodes an enzyme that decomposes D-cysteine into pyruvate, H2S, and NH3. Only D-cysteine but not L-cysteine was converted by D-CDes to pyruvate, H2S, and NH3. There is conflicting evidence on its 1-aminocyclopropane-1-carboxylate deaminase activity. Involved in regulating ethylene levels.
Computational Description: D-cysteine desulfhydrase (D-CDES); FUNCTIONS IN: 1-aminocyclopropane-1-carboxylate deaminase activity, D-cysteine desulfhydrase activity, cobalt ion binding, catalytic activity; INVOLVED IN: response to cadmium ion, ethylene biosynthetic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent enzyme, beta subunit (InterPro:IPR001926), Pyridoxal phosphate-dependent deaminase (InterPro:IPR005966); BEST Arabidopsis thaliana protein match is: Pyridoxal-5'-phosphate-dependent enzyme family protein (TAIR:AT3G26115.1); Has 2814 Blast hits to 2813 proteins in 938 species: Archae - 31; Bacteria - 1992; Metazoa - 30; Fungi - 102; Plants - 101; Viruses - 0; Other Eukaryotes - 558 (source: NCBI BLink).
Link: InterPro Scan - TAIR