F09732

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F09732

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F09732

Determination of introduced cDNA(s)

  • RAFL08-16-P09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL08-16-P09 - Resource: pda04927

    full reading [+] show sequence

    Nucleotide sequence: RAFL08-16-P09 - full reading
    GAGAGGACGA AGCTTGACAC TCTCAAGAGT AAAGCTCGAG CTTGCGAGAA 
    GAAGCATGTC TGCAACATCC GTACCTTCAA TGGCGGATTT TCTCACCAAA
    AAACCTTACT CTCCTCCTTC TTGGGCTTCT CATCTTCGTC CGCTTCCTTC
    TCACACTTTC TCCCTCGCTC ACCTTCCTAC TCCGATCCAT CGATGGAATC
    TTCCTGGTCT TCCTAATGGC ACAGAACTCT GGATCAAGCG AGATGATTTC
    ACCGGAATGG AATTGAGTGG AAACAAAGTA CGAAAACTCG AATTCTTAAT
    GGCGGAAGCT GTTGATCAAC ACGCTGATAC TGTAATCACT ATCGGCGGTA
    TTCAGAGCAA TCATTGTCGT GCTACAGCCA CTGCATCTAA CTATCTTAAT
    CTCAATTCTC ATCTTATTCT CCGTACTTCC AAGCTTCTTG CTGATGAAGA
    TCCTGGATTG GTTGGGAATC TCCTTGTCGA GCGTCTCGTT GGAGCTAATG
    TTCATCTAAT CTCTAAAGAA GAGTATTCTT CCATTGGGAG TGAGGCTCTT
    ACTAATGCTC TGAAAGAGAA ACTGGAAAAA GAAGGAAAGA AACCCTATGT
    TATTCCAGTC GGTGGATCGA ACTCTTTGGG AACTTGGGGT TATATAGAAG
    CAGCAAGGGA AATTGAGGAG CAGCTGAATT ATAGACCCGA TGACCTGAAA
    TTTGATGATA TTGTGGTAGC ATGTGGCAGT GGTGGTACAA TTGCTGGTAT
    TTCATTGGGG TCTTGGTTGG GAGCTCTAAA AGCCAAGGTT CATGCTTTCT
    CGGTTTGCGA TGATCCTGAT TACTTCTATG ACTTTGTCCA AGGGCTTCTG
    GATGGACTTC ACGCTGGTGT TAACTCTCGT GATATCGTCA ACATCCACAA
    TGCCAAAGGA AAAGGATATG CCATGAACAC GTCAGAGGAG CTTGAGTTTG
    TAAAGAAAGT AGCAAGTTCA ACTGGTGTTA TTCTTGATCC GGTTTACAGT
    GGGAAAGCTG CGTATGGTTT GATAAATGAG ATCACCAAAG ATCCCAAATG
    TTGGGAGGGA AGGAAGATAT TGTTCATACA CACTGGTGGG CTTCTTGGGT
    TGTATGATAA GGTTGATCAA ATGGCATCTC TGATGGGTAA TTGGTCCCGG
    ATGGATGTTT CAGAATCCGT TCCAAGAAAA GATGGTGTTG GGAAAATGTT
    CTAGCACAAC AAAATCTGAT TTGTAATAAT CCTGTGAAAA TAAGACAGAT
    TGAACAATTT GTGCATTTGG CAGTGAATAA ACACATCTCT GTTTT

    Gene models with high sequence identity

    1. AT1G48420.1 [+] show detail - D-cysteine desulfhydrase
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 1.2e-55
    HMMPfam Pyridoxal phosphate-dependent enzyme, beta subunit 8.5e-27
    HMMTigr Pyridoxal phosphate-dependent deaminase 2.8e-239
    HMMPanther 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE 1.5e-157
    HMMPanther SER/THR DEHYDRATASE, TRP SYNTHASE 1.5e-157
    superfamily Pyridoxal phosphate-dependent enzyme, beta subunit 8.5e-66

    See the detailed result >

    AT1G48420.1

    Model type
    Protein coding
    Short Description
    D-cysteine desulfhydrase
    Curator Summary
    Encodes an enzyme that decomposes D-cysteine into pyruvate, H2S, and NH3. Only D-cysteine but not L-cysteine was converted by D-CDes to pyruvate, H2S, and NH3. There is conflicting evidence on its 1-aminocyclopropane-1-carboxylate deaminase activity. Involved in regulating ethylene levels.
    Computational Description
    D-cysteine desulfhydrase (D-CDES); FUNCTIONS IN: 1-aminocyclopropane-1-carboxylate deaminase activity, D-cysteine desulfhydrase activity, cobalt ion binding, catalytic activity; INVOLVED IN: response to cadmium ion, ethylene biosynthetic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent enzyme, beta subunit (InterPro:IPR001926), Pyridoxal phosphate-dependent deaminase (InterPro:IPR005966); BEST Arabidopsis thaliana protein match is: Pyridoxal-5'-phosphate-dependent enzyme family protein (TAIR:AT3G26115.1); Has 2814 Blast hits to 2813 proteins in 938 species: Archae - 31; Bacteria - 1992; Metazoa - 30; Fungi - 102; Plants - 101; Viruses - 0; Other Eukaryotes - 558 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR