F10130

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10130

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F10130

Determination of introduced cDNA(s)

  • RAFL04-16-G24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-16-G24 - Resource: pda02325

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-16-G24 - full reading
    ATTTTGTCTT AACGACTCTT TATTGTCCCG ACACACGAAA ATGGCGTCTC 
    TTAGCCAAAT CACCCTGCCA CGCGCGCCGT CGTCGGAGAT TGGCCTCTTA
    CGCCGTCGAT TGGAGCGGCC GATCATCAGA ACCCGGATCG GATTCAACGG
    CCGGATTGCG AGCGTATTGA CTAACGCAGG GGACCAAGCG GTGTCGGTAA
    AAGCGAGTGT GAGCCAGAAG GTGATTGAAG AGGAAGCCAA AGTTATTGTG
    GGTACCTACG CGCGAGCTCC AGTGGTTCTC TCGAGTGGGA AAGGTTGTAA
    ATTGTTCGAC CCTGAAGGAA AAGAGTATCT GGATTGTGCA TCTGGAATCG
    CTGTGAATGC TCTTGGTCAT GGAGATCCTG ATTGGCTTCG AGCTGTCACT
    GAGCAAGCTG GTGTTCTTGC CCATGTCAGC AATGTTTATT ACACGATCCC
    TCAGATAGAG CTCGCAAAAC GCTTGGTGGC GAGTTCTTTT GCAGACCGTG
    TATTCTTTTG TAACTCGGGA ACGGAAGCCA ATGAAGCAGC CATTAAGTTT
    TCAAGAAAAT TCCAGAGATT CACCCATCCA GAAGACAAAG AAGTCGCGAC
    AGGTTTCATC GCCTTTACAA ACAGCTTCCA TGGAAGAACC TTAGGAGCTC
    TTGCCTTGAC AAGCAAAGAA CAATACAGAA CTCCTTTCGA ACCCATCATG
    CCTGGTGTAA CCTTCTTAGA ATATGGTAAC ATTCAAGCAG CCACAGACCT
    TATCCGCTCA GGTAAAATAG CTGCAGTTTT TGTGGAACCA ATTCAAGGAG
    AAGGCGGGAT TTACTCTGCT ACAAAAGAGT TTCTGCAATC TCTGCGCTCT
    GCTTGTGATG CCGCTGGATC TCTTCTCGTC TTCGATGAGG TTCAATGTGG
    TTTGGGTCGA ACGGGTCTCA TGTGGGCTTA TGAAGCCTTC GGTGTGACTC
    CTGACATAAT GACGGTTGCA AAGCCTTTAG CTGGTGGTTT ACCTATTGGA
    GCGGTGCTGG TGACTGAAAA GGTTGCAGAG ACCATAAACT ACGGAGATCA
    TGGAAGTACA TTTGCAGGCA GCCCTCTTGT GTGCAGTGCA GCCATTGCAG
    TTATGGATAA AGTATCGAAA CCCTCTTTCT TGTCCAGTGT CTCAAATAAA
    GGTCGATACT TTAGAGACCT GTTGGTGAAA AAACTTGGAG GAAACTCGCA
    CGTGAAGGAA GTGAGAGGCG AAGGGCTTAT CATAGGAGTG GAGCTCGATG
    TGCCGGCAAG CTCACTGGTT GATGCTTGCC GAGATTCGGG TCTTCTGATC
    TTGACAGCGG GGAAAGGAAA TGTCGTCAGG ATTGTTCCTC CGTTGGTTAT
    ATCAGAGGAG GAAATCGAAC GTGCGGTTGA GATCATGTCT CAAAATTTGA
    CTGCGCTTGA TTGAAGTTCT TGGAAGAGAA ATGATGTGGT GTTGAAGAAG
    AGCTAATGTT TTATATTCGA TCTTGTCTTA AATTAGCCTC AAGTGTACCG
    GTAAAGATGC TCTGCACAAT TGAAAAGAAA TAAAATTTGA CACAAAACTT
    GATGAACTAA TTGTTCAATC T

    Gene models with high sequence identity

    1. AT1G80600.1 [+] show detail - HOPW1-1-interacting 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Aminotransferase class-III 0
    superfamily Pyridoxal phosphate-dependent transferase, major domain 1e-125
    HMMTigr Acetylornithine/succinylornithine aminotransferase 7.9e-182
    HMMPfam Aminotransferase class-III 3.3e-99
    HMMPanther Acetylornithine/succinylornithine aminotransferase 6.1e-206
    HMMPanther Aminotransferase class-III 6.1e-206
    HAMAP ARGD_MF_01107 43.721
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 2 7e-07
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 8.3e-78

    See the detailed result >

    AT1G80600.1

    Model type
    Protein coding
    Short Description
    HOPW1-1-interacting 1
    Curator Summary
    Encodes HopW1-1-Interacting protein 1 (WIN1). Interacts with the P. syringae effector HopW1-1. WIN1 is a putative acetylornithine transaminase. Modulates plant defenses against bacteria. Three WIN proteins are identified so far (WIN1: AT1G80600; WIN2: AT4G31750; WIN3: AT5G13320).
    Computational Description
    HOPW1-1-interacting 1 (WIN1); FUNCTIONS IN: N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity, copper ion binding; INVOLVED IN: defense response to bacterium; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Acetylornithine/succinylornithine aminotransferase (InterPro:IPR004636), Aminotransferase class-III (InterPro:IPR005814), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: ornithine-delta-aminotransferase (TAIR:AT5G46180.1); Has 36928 Blast hits to 36904 proteins in 2775 species: Archae - 735; Bacteria - 23598; Metazoa - 655; Fungi - 914; Plants - 410; Viruses - 17; Other Eukaryotes - 10599 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR