F12143

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12143

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • present in fewer numbers in organism (PATO:0001997)
    (Cauline leaf::Number::few)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • decreased thickness (PATO:0000592)
    (Silique::Shape::thin)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12143

Determination of introduced cDNA(s)

  • RAFL07-07-M15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL07-07-M15 - Resource: pda03942

    full reading [+] show sequence

    Nucleotide sequence: RAFL07-07-M15 - full reading 
    GAGCTAACTA CCCAACCTGT GAAGCTACCC CCATTCTCTC TCAACGTTTT 
    CGTTTTGAAA TGGCGAGGAA GAAGATCAGA GAGTATGACT CAAAGAGGTT 
    GGTGAAGGAA CATTTCAAAA GGCTTTCTGG CAAAGAGCTT CCTATCAGAT 
    CCGTTCAGAT TAATGAAACA ACTGATCTAA ATGAGCTAGT TGAAAAGGAA 
    CCTTGGCTCT CGTCTGAGAA GCTGGTGGTG AAACCTGACA TGTTGTTTGG 
    AAAGCGTGGC AAGAGTGGTT TGGTTGCCTT GAAATTAGAT TTTGCTGATG 
    TTGCCACTTT TGTTAAAGAA CGTTTGGGAA AAGAGGTAGA GATGAGTGGA 
    TGCAAAGGAC CCATAACAAC ATTCATAGTT GAACCATTTG TTCCACACAA 
    TGAGGAGTAT TATCTCAATG TTGTCTCGGA TCGGCTTGGT TGCAGCATAA 
    GCTTTTCTGA GTGTGGAGGA ATTGAGATCG AGGAGAACTG GGACAAGGTC 
    AAGACAATAT TTTTACCAAC AGGTGCTTCC CTGACACCTG AAATATGTGC 
    ACCTCTTGTC GCAACTCTTC CCTTAGAGAT CAAAGCTGAA ATTGAAGAAT 
    TTATCAAAGT CATTTTCACC CTATTCCAAG ATCTTGATTT CACTTTCTTG 
    GAGATGAATC CTTTCACTCT AGTTGATGGA AGTCCTTATC CTCTGGATAT 
    GAGGGGTGAG CTTGATGATA CTGCTGCCTT CAAAAACTTT AAAAAATGGG 
    GCGACATTGA ATTTCCTCTG CCATTTGGAA GAGTAATGAG TCCTACAGAA 
    AGCTTTATCC ACGGACTGGA TGAGAAGACA AGTGCGTCTT TGAAGTTTAC 
    CGTTCTGAAC CCCAAGGGAC GGATTTGGAC AATGGTAGCT GGTGGAGGAG 
    CAAGTGTCAT CTATGCGGAT ACGGTTGGAG ATCTCGGGTA TGCATCTGAA 
    CTTGGCAACT ATGCTGAATA CAGTGGAGCA CCCAAAGAAG ATGAGGTTTT 
    GCAGTACGCC AGAGTCGTTA TTGATTGTGC TACAGCAAAC CCGGATGGAA 
    AAAGCAGAGC CCTTGTCATC GGAGGCGGAA TTGCCAACTT CACTGACGTT 
    GCTGCTACTT TCAATGGCAT AATCCGCGCT CTTAAAGAAA AGGAAGCAAA 
    GCTGAAAGCA GCAAGGATGC ATATATTTGT GAGGAGAGGA GGACCAAACT 
    ACCAAAAGGG ACTTGCTAAA ATGCGAGCCC TTGGAGATGA TATCGGTGTC 
    CCCATCGAGG TCTATGGCCC AGAAGCAACC ATGACAGGTA TCTGCAAGGA 
    GGCAATCCAG TACATCACAG CAGCAGCATA AGCTTCTCTC AGTACCTTCT 
    ATGACCAAAA CTTTGTCTGT GTTTTAGAGC CTTTATTTAC TGTGGTTAAG 
    ATTACTCAAG CTATAAGATA CTTGCAATTT CTTGAAAACT TCTGTTGTTC 
    GATTCTCTTT CCCCTAACGT TTTCTTCAGA TTCAATAAAT AATCGTTACT 
    TCTAAAAAAA AAAAAAAAA

    Gene models with high sequence identity

    1. AT1G10670.1 [+] show detail - ATP-citrate lyase A-1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam ATP-grasp fold, succinyl-CoA synthetase-type 9.5e-17
    superfamily Glutathione synthetase ATP-binding domain-lik 5e-37
    superfamily Succinyl-CoA synthetase-like 2.6e-18
    HMMPanther ATP-CITRATE SYNTHASE 1.1e-166
    Gene3D ATP-grasp fold, subdomain 2 4.2e-17

    See the detailed result >

    AT1G10670.1

    Model type
    Protein coding
    Short Description
    ATP-citrate lyase A-1
    Curator Summary
    One of the three genes encoding subunit A of the trimeric protein ATP Citrate Lyase. Antisense ACLA-1 plants cause a reduction in cytosolic acetyl-CoA metabolism and have upregulation of stress-related genes and down-regulation of primary metabolism and growth genes, suggesting the mutation restricts normal growth and developmental processes and puts the plant into a state of stress.
    Computational Description
    ATP-citrate lyase A-1 (ACLA-1); CONTAINS InterPro DOMAIN/s: ATP-grasp fold, subdomain 2 (InterPro:IPR013816), ATP-grasp fold, succinyl-CoA synthetase-type (InterPro:IPR013650), Succinyl-CoA synthetase-like (InterPro:IPR016102); BEST Arabidopsis thaliana protein match is: ATP-citrate lyase A-2 (TAIR:AT1G60810.1); Has 5465 Blast hits to 5464 proteins in 1555 species: Archae - 120; Bacteria - 3046; Metazoa - 362; Fungi - 200; Plants - 107; Viruses - 0; Other Eukaryotes - 1630 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR