F12143

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12143

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • present in fewer numbers in organism (PATO:0001997)
    (Cauline leaf::Number::few)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • decreased thickness (PATO:0000592)
    (Silique::Shape::thin)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12143

Determination of introduced cDNA(s)

  • RAFL07-07-M15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL07-07-M15 - Resource: pda03942

    full reading [+] show sequence

    Nucleotide sequence: RAFL07-07-M15 - full reading
    GAGCTAACTA CCCAACCTGT GAAGCTACCC CCATTCTCTC TCAACGTTTT 
    CGTTTTGAAA TGGCGAGGAA GAAGATCAGA GAGTATGACT CAAAGAGGTT
    GGTGAAGGAA CATTTCAAAA GGCTTTCTGG CAAAGAGCTT CCTATCAGAT
    CCGTTCAGAT TAATGAAACA ACTGATCTAA ATGAGCTAGT TGAAAAGGAA
    CCTTGGCTCT CGTCTGAGAA GCTGGTGGTG AAACCTGACA TGTTGTTTGG
    AAAGCGTGGC AAGAGTGGTT TGGTTGCCTT GAAATTAGAT TTTGCTGATG
    TTGCCACTTT TGTTAAAGAA CGTTTGGGAA AAGAGGTAGA GATGAGTGGA
    TGCAAAGGAC CCATAACAAC ATTCATAGTT GAACCATTTG TTCCACACAA
    TGAGGAGTAT TATCTCAATG TTGTCTCGGA TCGGCTTGGT TGCAGCATAA
    GCTTTTCTGA GTGTGGAGGA ATTGAGATCG AGGAGAACTG GGACAAGGTC
    AAGACAATAT TTTTACCAAC AGGTGCTTCC CTGACACCTG AAATATGTGC
    ACCTCTTGTC GCAACTCTTC CCTTAGAGAT CAAAGCTGAA ATTGAAGAAT
    TTATCAAAGT CATTTTCACC CTATTCCAAG ATCTTGATTT CACTTTCTTG
    GAGATGAATC CTTTCACTCT AGTTGATGGA AGTCCTTATC CTCTGGATAT
    GAGGGGTGAG CTTGATGATA CTGCTGCCTT CAAAAACTTT AAAAAATGGG
    GCGACATTGA ATTTCCTCTG CCATTTGGAA GAGTAATGAG TCCTACAGAA
    AGCTTTATCC ACGGACTGGA TGAGAAGACA AGTGCGTCTT TGAAGTTTAC
    CGTTCTGAAC CCCAAGGGAC GGATTTGGAC AATGGTAGCT GGTGGAGGAG
    CAAGTGTCAT CTATGCGGAT ACGGTTGGAG ATCTCGGGTA TGCATCTGAA
    CTTGGCAACT ATGCTGAATA CAGTGGAGCA CCCAAAGAAG ATGAGGTTTT
    GCAGTACGCC AGAGTCGTTA TTGATTGTGC TACAGCAAAC CCGGATGGAA
    AAAGCAGAGC CCTTGTCATC GGAGGCGGAA TTGCCAACTT CACTGACGTT
    GCTGCTACTT TCAATGGCAT AATCCGCGCT CTTAAAGAAA AGGAAGCAAA
    GCTGAAAGCA GCAAGGATGC ATATATTTGT GAGGAGAGGA GGACCAAACT
    ACCAAAAGGG ACTTGCTAAA ATGCGAGCCC TTGGAGATGA TATCGGTGTC
    CCCATCGAGG TCTATGGCCC AGAAGCAACC ATGACAGGTA TCTGCAAGGA
    GGCAATCCAG TACATCACAG CAGCAGCATA AGCTTCTCTC AGTACCTTCT
    ATGACCAAAA CTTTGTCTGT GTTTTAGAGC CTTTATTTAC TGTGGTTAAG
    ATTACTCAAG CTATAAGATA CTTGCAATTT CTTGAAAACT TCTGTTGTTC
    GATTCTCTTT CCCCTAACGT TTTCTTCAGA TTCAATAAAT AATCGTTACT
    TCTAAAAAAA AAAAAAAAA

    Gene models with high sequence identity

    1. AT1G10670.1 [+] show detail - ATP-citrate lyase A-1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam ATP-grasp fold, succinyl-CoA synthetase-type 9.5e-17
    superfamily Glutathione synthetase ATP-binding domain-lik 5e-37
    superfamily Succinyl-CoA synthetase-like 2.6e-18
    HMMPanther ATP-CITRATE SYNTHASE 1.1e-166
    Gene3D ATP-grasp fold, subdomain 2 4.2e-17

    See the detailed result >

    AT1G10670.1

    Model type
    Protein coding
    Short Description
    ATP-citrate lyase A-1
    Curator Summary
    One of the three genes encoding subunit A of the trimeric protein ATP Citrate Lyase. Antisense ACLA-1 plants cause a reduction in cytosolic acetyl-CoA metabolism and have upregulation of stress-related genes and down-regulation of primary metabolism and growth genes, suggesting the mutation restricts normal growth and developmental processes and puts the plant into a state of stress.
    Computational Description
    ATP-citrate lyase A-1 (ACLA-1); CONTAINS InterPro DOMAIN/s: ATP-grasp fold, subdomain 2 (InterPro:IPR013816), ATP-grasp fold, succinyl-CoA synthetase-type (InterPro:IPR013650), Succinyl-CoA synthetase-like (InterPro:IPR016102); BEST Arabidopsis thaliana protein match is: ATP-citrate lyase A-2 (TAIR:AT1G60810.1); Has 5465 Blast hits to 5464 proteins in 1555 species: Archae - 120; Bacteria - 3046; Metazoa - 362; Fungi - 200; Plants - 107; Viruses - 0; Other Eukaryotes - 1630 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR