F13140

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F13140

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
T2  
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
  • abnormal (PATO:0000460)
    (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13140

Determination of introduced cDNA(s)

  • RAFL06-81-G12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-81-G12 - Resource: pda03804

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-81-G12 - full reading 
    ATGAAAGTTT TATGCTTTCG ATGATTCCCA AAATCTTTCT CATGCGTTTT 
    TTACTTTCTT GATTTGGCAA TCCTCATGAG CTGATTTTGA TTTCTTCTTT 
    ATGGTTGCCG ACACGAAAGA AGAAGCTAGG ACACAAAACA ACTCAAATTT 
    TGTTTGGTGA GAAAAATCAA CCAAGAAAAT ATTGATTCTT TTGGAGCAAG 
    GAGTTGGTTT GATTGGTAAT GCTCAAGTTG GTCTCTCTTT TTTCTTTTCA 
    AATCCAACTT GATTTCTTTG TTCTATATGA TCCAAACCCA TGTAGTATAA 
    ATCTGTAGTG GATTACATAA CCACCAGAAA AAATCATTTC TAGTCATTGT 
    TCATTATTGT CTTTGGTTTG TTTGAACTTT CTTGTGTGTG TGGACCATAA 
    GAAGAAGGAA TGGTGGGAAG CATTGAGGCA AAGAGTCTGC AATCAAACGG 
    GTCTGTTCAT CATATTGGTC TTAATTTGGA GGAGAAACTT GATGAATTTC 
    GTCGTCTTTT GGGGAAATCA GAAAAAGATC CGTTAAGGAT TGTAAGTGTT 
    GGTGCTGGTG CTTGGGGAAG TGTTTTTGCA GCACTTCTTC AAGAAAGCTA 
    TGGAGGTTTC AGGGATAAGT TTCAGATCAG GATATGGAGA AGAGCTGGGA 
    GAGCTGTTGA TAGAGAAACT GCAGAACATT TGTTTGAAGT GATCAATTCA 
    AGGGAAGATA TCTTGAGGAG ATTGATAAGA CGCTGTGCTT ATCTGAAATA 
    TGTCGAGGCA AGGCTTGGTG ATAGGACACT CTATGCTGAT GAGATATTGA 
    AAGACGGGTT TTGTCTTAAC ATGGTTGATA CGCCGCTTTG TCCTCTTAAG 
    GTTGTGACAA ATCTGCAAGA AGCTGTGTGG GATGCTGATA TTGTTGTTAA 
    TGGATTGCCT TCAACTGAAA CACGTGAAGT GTTTGAAGAG ATTAGTAAGT 
    ATTGGAAAGA GAGAATAACG GTTCCGATTA TTATCTCTCT GTCAAAGGGT 
    ATTGAAACTG CTCTTGAACC AGTTCCACAT ATCATAACTC CAACAAAGAT 
    GATTCATCAA GCAACTGGTG TGCCGATTGA CAATGTCCTG TATCTTGGTG 
    GACCAAACAT TGCTGCTGAA ATTTACAACA AGGAATATGC CAATGCTAGA 
    ATCTGTGGAG CTGCTAAATG GAGGAAGCCA CTAGCTAAGT TCTTAAGACA 
    ACCTCATTTC ATTGTTTGGG ACAATAGTGA TCTTGTGACA CATGAAGTAA 
    TGGGAGGTCT CAAGAATGTC TACGCCATTG GAGCTGGTAT GGTAGCGGCG 
    CTCACTAACG AGAGCGCTAC AAGCAAGTCG GTGTATTTTG CTCATTGTAC 
    ATCTGAGATG ATATTTATAA CTCATTTACT AGCAGAAGAG CCTGAGAAAC 
    TTGCAGGGCC TTTGCTAGCT GACACTTATG TGACCTTATT AAAAGGACGT 
    AATGCATGGT ACGGTCAAAT GCTGGCAAAG GGTGAAATAA ATAGAGACAT 
    GGGTGATAGC ATAAGCGGCA AGGGAATGAT TCAGGGTGTT TCTGCAGTGG 
    GAGCATTTTA CCAATTGCTT AGTCAATCAA GCTTAAGTAT ATTGCCCTCT 
    GAAGAGAAGA AACCTGTAGC TCCGGTCGAA TCATGTCCTA TTTTAAAGAC 
    ACTTTACAAG ATACTCATCA CAAGAGAACA ATCAACTCAG GCCATTCTGC 
    AAGCATTAAG GGATGAGACA TTGAACGATC CCAGAGACCG TATTGAGATT 
    GCACAGAGCC ATGCATTCTA CAGGCCTTCC CTTCTTGGTC AGCCTTGATT 
    AGTCAGTCAT GGCTTTTATA AGAGATTCAA CATAAATTAC TTCTGCAAGC 
    TGCAGATGCT TCATTCAAAA CATTATAGCT TTAATGGGAA GGGGTAGTTT 
    CACATCTGTC ATAACTTAAA ATGGATTCTT GTTAAGTCTC TGAGTCAATG 
    CAATCTTTGT ACTGTATCAT TTTCTTTATG ATCAATGAGA GCTTTATTTG 
    CTCCATAAAA AAAAAAAAAA AAA

    Gene models with high sequence identity

    1. AT2G41540.2 [+] show detail - 6-phosphogluconate dehydrogenase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D NAD(P)-binding domain 3.1e-08
    Gene3D Dehydrogenase, multihelical 4.3e-17
    superfamily 6-phosphogluconate dehydrogenase, C-terminal-like 1e-18
    superfamily NAD(P)-binding domain 9.6e-15
    HMMPfam NAD-dependent glycerol-3-phosphate dehydrogenase, C-terminal 8.3e-40
    HMMPfam NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal 2.5e-10
    FPrintScan NAD-dependent glycerol-3-phosphate dehydrogenase 6.7e-07
    FPrintScan NAD-dependent glycerol-3-phosphate dehydrogenase 6.7e-07
    HMMPanther NAD-dependent glycerol-3-phosphate dehydrogenase 2.4e-98

    See the detailed result >

    AT2G41540.2

    Model type
    Protein coding
    Short Description
    6-phosphogluconate dehydrogenase family protein
    Curator Summary
    Encodes a protein with NAD-dependent glycerol-3-phosphate (G3P) dehydrogenase which was shown to complement an Escherichia coli strain: BB20-14, auxotrophic for glycerol/G3P due to a loss-of-function mutation in the gpsA gene.
    Computational Description
    GPDHC1; CONTAINS InterPro DOMAIN/s: 6-phosphogluconate dehydrogenase, C-terminal-like (InterPro:IPR008927), Dehydrogenase, multihelical (InterPro:IPR013328), NAD(P)-binding domain (InterPro:IPR016040), NAD-dependent glycerol-3-phosphate dehydrogenase, C-terminal (InterPro:IPR006109), NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal (InterPro:IPR011128), NAD-dependent glycerol-3-phosphate dehydrogenase (InterPro:IPR006168); BEST Arabidopsis thaliana protein match is: 6-phosphogluconate dehydrogenase family protein (TAIR:AT3G07690.1); Has 4956 Blast hits to 4956 proteins in 1719 species: Archae - 20; Bacteria - 3172; Metazoa - 444; Fungi - 126; Plants - 154; Viruses - 0; Other Eukaryotes - 1040 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR