F13140

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F13140

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
T2  
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
  • abnormal (PATO:0000460)
    (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13140

Determination of introduced cDNA(s)

  • RAFL06-81-G12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-81-G12 - Resource: pda03804

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-81-G12 - full reading
    ATGAAAGTTT TATGCTTTCG ATGATTCCCA AAATCTTTCT CATGCGTTTT 
    TTACTTTCTT GATTTGGCAA TCCTCATGAG CTGATTTTGA TTTCTTCTTT
    ATGGTTGCCG ACACGAAAGA AGAAGCTAGG ACACAAAACA ACTCAAATTT
    TGTTTGGTGA GAAAAATCAA CCAAGAAAAT ATTGATTCTT TTGGAGCAAG
    GAGTTGGTTT GATTGGTAAT GCTCAAGTTG GTCTCTCTTT TTTCTTTTCA
    AATCCAACTT GATTTCTTTG TTCTATATGA TCCAAACCCA TGTAGTATAA
    ATCTGTAGTG GATTACATAA CCACCAGAAA AAATCATTTC TAGTCATTGT
    TCATTATTGT CTTTGGTTTG TTTGAACTTT CTTGTGTGTG TGGACCATAA
    GAAGAAGGAA TGGTGGGAAG CATTGAGGCA AAGAGTCTGC AATCAAACGG
    GTCTGTTCAT CATATTGGTC TTAATTTGGA GGAGAAACTT GATGAATTTC
    GTCGTCTTTT GGGGAAATCA GAAAAAGATC CGTTAAGGAT TGTAAGTGTT
    GGTGCTGGTG CTTGGGGAAG TGTTTTTGCA GCACTTCTTC AAGAAAGCTA
    TGGAGGTTTC AGGGATAAGT TTCAGATCAG GATATGGAGA AGAGCTGGGA
    GAGCTGTTGA TAGAGAAACT GCAGAACATT TGTTTGAAGT GATCAATTCA
    AGGGAAGATA TCTTGAGGAG ATTGATAAGA CGCTGTGCTT ATCTGAAATA
    TGTCGAGGCA AGGCTTGGTG ATAGGACACT CTATGCTGAT GAGATATTGA
    AAGACGGGTT TTGTCTTAAC ATGGTTGATA CGCCGCTTTG TCCTCTTAAG
    GTTGTGACAA ATCTGCAAGA AGCTGTGTGG GATGCTGATA TTGTTGTTAA
    TGGATTGCCT TCAACTGAAA CACGTGAAGT GTTTGAAGAG ATTAGTAAGT
    ATTGGAAAGA GAGAATAACG GTTCCGATTA TTATCTCTCT GTCAAAGGGT
    ATTGAAACTG CTCTTGAACC AGTTCCACAT ATCATAACTC CAACAAAGAT
    GATTCATCAA GCAACTGGTG TGCCGATTGA CAATGTCCTG TATCTTGGTG
    GACCAAACAT TGCTGCTGAA ATTTACAACA AGGAATATGC CAATGCTAGA
    ATCTGTGGAG CTGCTAAATG GAGGAAGCCA CTAGCTAAGT TCTTAAGACA
    ACCTCATTTC ATTGTTTGGG ACAATAGTGA TCTTGTGACA CATGAAGTAA
    TGGGAGGTCT CAAGAATGTC TACGCCATTG GAGCTGGTAT GGTAGCGGCG
    CTCACTAACG AGAGCGCTAC AAGCAAGTCG GTGTATTTTG CTCATTGTAC
    ATCTGAGATG ATATTTATAA CTCATTTACT AGCAGAAGAG CCTGAGAAAC
    TTGCAGGGCC TTTGCTAGCT GACACTTATG TGACCTTATT AAAAGGACGT
    AATGCATGGT ACGGTCAAAT GCTGGCAAAG GGTGAAATAA ATAGAGACAT
    GGGTGATAGC ATAAGCGGCA AGGGAATGAT TCAGGGTGTT TCTGCAGTGG
    GAGCATTTTA CCAATTGCTT AGTCAATCAA GCTTAAGTAT ATTGCCCTCT
    GAAGAGAAGA AACCTGTAGC TCCGGTCGAA TCATGTCCTA TTTTAAAGAC
    ACTTTACAAG ATACTCATCA CAAGAGAACA ATCAACTCAG GCCATTCTGC
    AAGCATTAAG GGATGAGACA TTGAACGATC CCAGAGACCG TATTGAGATT
    GCACAGAGCC ATGCATTCTA CAGGCCTTCC CTTCTTGGTC AGCCTTGATT
    AGTCAGTCAT GGCTTTTATA AGAGATTCAA CATAAATTAC TTCTGCAAGC
    TGCAGATGCT TCATTCAAAA CATTATAGCT TTAATGGGAA GGGGTAGTTT
    CACATCTGTC ATAACTTAAA ATGGATTCTT GTTAAGTCTC TGAGTCAATG
    CAATCTTTGT ACTGTATCAT TTTCTTTATG ATCAATGAGA GCTTTATTTG
    CTCCATAAAA AAAAAAAAAA AAA

    Gene models with high sequence identity

    1. AT2G41540.2 [+] show detail - 6-phosphogluconate dehydrogenase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D NAD(P)-binding domain 3.1e-08
    Gene3D Dehydrogenase, multihelical 4.3e-17
    superfamily 6-phosphogluconate dehydrogenase, C-terminal-like 1e-18
    superfamily NAD(P)-binding domain 9.6e-15
    HMMPfam NAD-dependent glycerol-3-phosphate dehydrogenase, C-terminal 8.3e-40
    HMMPfam NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal 2.5e-10
    FPrintScan NAD-dependent glycerol-3-phosphate dehydrogenase 6.7e-07
    FPrintScan NAD-dependent glycerol-3-phosphate dehydrogenase 6.7e-07
    HMMPanther NAD-dependent glycerol-3-phosphate dehydrogenase 2.4e-98

    See the detailed result >

    AT2G41540.2

    Model type
    Protein coding
    Short Description
    6-phosphogluconate dehydrogenase family protein
    Curator Summary
    Encodes a protein with NAD-dependent glycerol-3-phosphate (G3P) dehydrogenase which was shown to complement an Escherichia coli strain: BB20-14, auxotrophic for glycerol/G3P due to a loss-of-function mutation in the gpsA gene.
    Computational Description
    GPDHC1; CONTAINS InterPro DOMAIN/s: 6-phosphogluconate dehydrogenase, C-terminal-like (InterPro:IPR008927), Dehydrogenase, multihelical (InterPro:IPR013328), NAD(P)-binding domain (InterPro:IPR016040), NAD-dependent glycerol-3-phosphate dehydrogenase, C-terminal (InterPro:IPR006109), NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal (InterPro:IPR011128), NAD-dependent glycerol-3-phosphate dehydrogenase (InterPro:IPR006168); BEST Arabidopsis thaliana protein match is: 6-phosphogluconate dehydrogenase family protein (TAIR:AT3G07690.1); Has 4956 Blast hits to 4956 proteins in 1719 species: Archae - 20; Bacteria - 3172; Metazoa - 444; Fungi - 126; Plants - 154; Viruses - 0; Other Eukaryotes - 1040 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR