F13140

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) increased width (PATO:0000600) (Rosette leaf::Shape::wide)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin) abnormal (PATO:0000460) (Stem::Shape::zigzag)
T2
	rosette leaf (PO:0000014)	increased width (PATO:0000600) (Rosette leaf::Shape::wide)
	fruit (PO:0009001)	decreased length (PATO:0000574) (Silique::Shape::short) abnormal (PATO:0000460) (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13140

Determination of introduced cDNA(s)

RAFL06-81-G12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-81-G12 - Resource: pda03804

full reading [+] show sequence

ATGAAAGTTT TATGCTTTCG ATGATTCCCA AAATCTTTCT CATGCGTTTT 
TTACTTTCTT GATTTGGCAA TCCTCATGAG CTGATTTTGA TTTCTTCTTT 
ATGGTTGCCG ACACGAAAGA AGAAGCTAGG ACACAAAACA ACTCAAATTT 
TGTTTGGTGA GAAAAATCAA CCAAGAAAAT ATTGATTCTT TTGGAGCAAG 
GAGTTGGTTT GATTGGTAAT GCTCAAGTTG GTCTCTCTTT TTTCTTTTCA 
AATCCAACTT GATTTCTTTG TTCTATATGA TCCAAACCCA TGTAGTATAA 
ATCTGTAGTG GATTACATAA CCACCAGAAA AAATCATTTC TAGTCATTGT 
TCATTATTGT CTTTGGTTTG TTTGAACTTT CTTGTGTGTG TGGACCATAA 
GAAGAAGGAA TGGTGGGAAG CATTGAGGCA AAGAGTCTGC AATCAAACGG 
GTCTGTTCAT CATATTGGTC TTAATTTGGA GGAGAAACTT GATGAATTTC 
GTCGTCTTTT GGGGAAATCA GAAAAAGATC CGTTAAGGAT TGTAAGTGTT 
GGTGCTGGTG CTTGGGGAAG TGTTTTTGCA GCACTTCTTC AAGAAAGCTA 
TGGAGGTTTC AGGGATAAGT TTCAGATCAG GATATGGAGA AGAGCTGGGA 
GAGCTGTTGA TAGAGAAACT GCAGAACATT TGTTTGAAGT GATCAATTCA 
AGGGAAGATA TCTTGAGGAG ATTGATAAGA CGCTGTGCTT ATCTGAAATA 
TGTCGAGGCA AGGCTTGGTG ATAGGACACT CTATGCTGAT GAGATATTGA 
AAGACGGGTT TTGTCTTAAC ATGGTTGATA CGCCGCTTTG TCCTCTTAAG 
GTTGTGACAA ATCTGCAAGA AGCTGTGTGG GATGCTGATA TTGTTGTTAA 
TGGATTGCCT TCAACTGAAA CACGTGAAGT GTTTGAAGAG ATTAGTAAGT 
ATTGGAAAGA GAGAATAACG GTTCCGATTA TTATCTCTCT GTCAAAGGGT 
ATTGAAACTG CTCTTGAACC AGTTCCACAT ATCATAACTC CAACAAAGAT 
GATTCATCAA GCAACTGGTG TGCCGATTGA CAATGTCCTG TATCTTGGTG 
GACCAAACAT TGCTGCTGAA ATTTACAACA AGGAATATGC CAATGCTAGA 
ATCTGTGGAG CTGCTAAATG GAGGAAGCCA CTAGCTAAGT TCTTAAGACA 
ACCTCATTTC ATTGTTTGGG ACAATAGTGA TCTTGTGACA CATGAAGTAA 
TGGGAGGTCT CAAGAATGTC TACGCCATTG GAGCTGGTAT GGTAGCGGCG 
CTCACTAACG AGAGCGCTAC AAGCAAGTCG GTGTATTTTG CTCATTGTAC 
ATCTGAGATG ATATTTATAA CTCATTTACT AGCAGAAGAG CCTGAGAAAC 
TTGCAGGGCC TTTGCTAGCT GACACTTATG TGACCTTATT AAAAGGACGT 
AATGCATGGT ACGGTCAAAT GCTGGCAAAG GGTGAAATAA ATAGAGACAT 
GGGTGATAGC ATAAGCGGCA AGGGAATGAT TCAGGGTGTT TCTGCAGTGG 
GAGCATTTTA CCAATTGCTT AGTCAATCAA GCTTAAGTAT ATTGCCCTCT 
GAAGAGAAGA AACCTGTAGC TCCGGTCGAA TCATGTCCTA TTTTAAAGAC 
ACTTTACAAG ATACTCATCA CAAGAGAACA ATCAACTCAG GCCATTCTGC 
AAGCATTAAG GGATGAGACA TTGAACGATC CCAGAGACCG TATTGAGATT 
GCACAGAGCC ATGCATTCTA CAGGCCTTCC CTTCTTGGTC AGCCTTGATT 
AGTCAGTCAT GGCTTTTATA AGAGATTCAA CATAAATTAC TTCTGCAAGC 
TGCAGATGCT TCATTCAAAA CATTATAGCT TTAATGGGAA GGGGTAGTTT 
CACATCTGTC ATAACTTAAA ATGGATTCTT GTTAAGTCTC TGAGTCAATG 
CAATCTTTGT ACTGTATCAT TTTCTTTATG ATCAATGAGA GCTTTATTTG 
CTCCATAAAA AAAAAAAAAA AAA

Gene models with high sequence identity

AT2G41540.2 [+] show detail - 6-phosphogluconate dehydrogenase family protein
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
Gene3D	NAD(P)-binding domain	0.000000031
Gene3D	Dehydrogenase, multihelical	4.3e-17
superfamily	6-phosphogluconate dehydrogenase, C-terminal-like	1e-18
superfamily	NAD(P)-binding domain	0.0000000000000096
HMMPfam	NAD-dependent glycerol-3-phosphate dehydrogenase, C-terminal	8.3e-40
HMMPfam	NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal	0.00000000025
FPrintScan	NAD-dependent glycerol-3-phosphate dehydrogenase	0.00000067
FPrintScan	NAD-dependent glycerol-3-phosphate dehydrogenase	0.00000067
HMMPanther	NAD-dependent glycerol-3-phosphate dehydrogenase	2.4e-98

See the detailed result >

AT2G41540.2

Model type: Protein coding
Short Description: 6-phosphogluconate dehydrogenase family protein
Curator Summary: Encodes a protein with NAD-dependent glycerol-3-phosphate (G3P) dehydrogenase which was shown to complement an Escherichia coli strain: BB20-14, auxotrophic for glycerol/G3P due to a loss-of-function mutation in the gpsA gene.
Computational Description: GPDHC1; CONTAINS InterPro DOMAIN/s: 6-phosphogluconate dehydrogenase, C-terminal-like (InterPro:IPR008927), Dehydrogenase, multihelical (InterPro:IPR013328), NAD(P)-binding domain (InterPro:IPR016040), NAD-dependent glycerol-3-phosphate dehydrogenase, C-terminal (InterPro:IPR006109), NAD-dependent glycerol-3-phosphate dehydrogenase, N-terminal (InterPro:IPR011128), NAD-dependent glycerol-3-phosphate dehydrogenase (InterPro:IPR006168); BEST Arabidopsis thaliana protein match is: 6-phosphogluconate dehydrogenase family protein (TAIR:AT3G07690.1); Has 4956 Blast hits to 4956 proteins in 1719 species: Archae - 20; Bacteria - 3172; Metazoa - 444; Fungi - 126; Plants - 154; Viruses - 0; Other Eukaryotes - 1040 (source: NCBI BLink).
Link: InterPro Scan - TAIR