F13213

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Seed::deep red

Original: F13213

Determination of introduced cDNA(s)

RAFL09-83-J08 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-83-J08 - Resource: pda08899

full reading [+] show sequence

TCCGAAGTTA ACAATGGTGG AATAGCGATT CTTCGACGAC GATGATGGCG 
AGGTTTGTTT CTGTGTCGTC TTGTCAGTTT CATTTCGGCT TCCGTGAGGT 
TTCTCCTCCG TCGGTAACTT CTTATCCTAG ACGATTCGAG GTCTCTGATC 
GGAGATTTCC TGCGATTCCG ATTAAATGTT CTTCATCTGA GCCGGAAAAC 
GGTGAGGATT CGGCACCGTC GCTGTCTTCC TCCTCCTCAT CGTCTACAAG 
TGAAGTATCT ACTAGTAACA GTTCGACATA CAACTGGTAC ACCGGAATCG 
GCGGTATTGG GATGTTAGAC ACTGCGTATT TGACTTACCT CAAGGTCACT 
GGCTCCGATG CGTTTTGCCC TATTGGTGGT GGCACTTGCG GTGATGTCCT 
CAACAGCGAT TATGCCGTCG TTTTCGGTGT TCCTCTTCCA GTGATTGGAT 
TTGTTATGTA TGGCGTAGTA ACCGCTCTAA GTGCAGAGCT TGGGGAAGGG 
AATCTACCTT TTGGAATCAG TAAGAGCAAT GGGCGTTTCG CGTTATTTGG 
GATCACGACT GCAATGGCAT CTGCTAGTGC ATATTTCCTG TATATCCTTA 
GCACAAAACT CTCAGGATCA TCATGCCTGT ATTGTCTGGT GTCTGCTTTC 
TTATCATTTA GTCTATTTTT CCTCTCTGTA AAGGATGTGA AGTTGCAAGA 
AATACAGCAA GTTGTAGGAT TGCAGATATG CTTAGCAATC ATAGTAGTTG 
CCTCCTTGAC TGCTTCTTAC AGTACTGCTC AACCAATCCC TTCACGCTCT 
GGTGACATTG AACTGCCATA TTTTAGAACA GAGATCAGTT CATCATCGAG 
CCCTTATGCT ATTGCTTTAG CAAAACATCT AAACTCCATT GGAGCTAAAA 
TGTATGGGGC ATTCTGGTGT TCTCACTGCT TAGAGCAAAA AGAGATGTTT 
GGAAGAGAAG CGGCAAAAGA ACTGAACTAC GTGGAATGTT TTCCGGATGG 
ATATAAGAAA GGAACTAAGA TACTCAAGGC ATGTGCAGAT GCTGCGATCG 
AAGGATTCCC AACATGGATA ATAAATGATA AGGTCTTAAG CGGAGAAATA 
GAACTCGCTG AACTAGCGGA GATGACTGGA TTCAGCCTTG ATCAGGCAAA 
TGAGACCAAT CAACTTCAGT AAATTTTGTA TTGGTGCAGA AAATATTAAG 
GTGAATCCGT AAATGTTGTT TACTTGATCC TCCTGATAGC TATAGGGTGT 
TCTATACAAA TTCAAGTAAT GAAAGTGCTT ATTGTTGCCA TAGGAGGTTA 
CAACTTCATC TGTATCACTT AGCCTACACC AGAGCATCAC TATTTTATAA 
TTGAAAGATT CATAGGTTCT TCCAAAGAAT GTTAGGGTAC TATTAATTCG 
AGAATCTACA GCCATCTAGT ACTGGCTTTA TGTAAGTATA ATGTCTAAAA 
GTATGGTCTA TATAAGAGAG TCACACATGT ATTTTGTATA TATATGCGTG 
TAGAGTAGTA GCAGATATAT GATGTATGGG AAACTTAGAG AATTACGTTT 
ACAATATTTG GTGCCCTATT GAATACCTTC CTT

Gene models with high sequence identity

AT4G35760.1 [+] show detail - NAD(P)H dehydrogenase (quinone)s
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Vitamin K epoxide reductase	6.6e-30
HMMSmart	Vitamin K epoxide reductase	3.7e-44
superfamily	Thioredoxin-like fold	4.8e-07

See the detailed result >

AT4G35760.1

Model type: Protein coding
Short Description: NAD(P)H dehydrogenase (quinone)s
Curator Summary: Encodes a bimodular enzyme comprising an integral domain homologous to the catalytic subunit of mammalian vitamin K epoxide reductase (VKORC1, EC 1.1.4.1) that is fused to a soluble thioredoxin-like moiety. Using yeast microsomes as a recombinant system, it was shown that the VKORC1 domain of At4g35760 functions as a stringent naphthoquinone reductase, and that its reduced Trx-like partner can serve as its electron donor. Located in plastid.
Computational Description: NAD(P)H dehydrogenase (quinone)s; FUNCTIONS IN: NAD(P)H dehydrogenase (quinone) activity; LOCATED IN: chloroplast, plastid; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Vitamin K epoxide reductase (InterPro:IPR012932), Thioredoxin-like fold (InterPro:IPR012336); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR