F16516

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F16516

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • present in fewer numbers in organism (PATO:0001997)
    (Cauline leaf::Number::few)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Other)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
T2  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F16516

Determination of introduced cDNA(s)

  • RAFL04-16-A19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-16-A19 - Resource: pda00605

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-16-A19 - full reading
    ACCATCTCCT TCAGATCCAC ACACTCTCTC TTATATCTCT CCGGCGAAAA 
    CTTTCCGATC ACCGACAAAA AAAAAAAATG GTTGCAGACG CAAACGGTTC
    ATCATCAAGC TCATTTAACT TCCTAATCTA CGGTAAAACC GGATGGATCG
    GTGGTTTACT CGGTAAACTC TGCGAAGCTC AAGGAATCAC TTACACTTAC
    GGCTCCGGTC GTCTTCAAGA TCGTCAATCG ATCGTCGCCG ACATCGAATC
    CGTGAAACCT AGCCACGTGT TCAACGCTGC TGGAGTCACC GGTCGTCCTA
    ATGTTGATTG GTGCGAATCT CACAAAGTTG AGACGATTCG TACTAATGTC
    GCCGGAACCC TAACTCTCGC TGACATTTGC AGAGAGAAAG GACTTGTTCT
    GATCAATTAC GCTACGGGTT GTATATTTGA GTATGATTCG GGTCATCATC
    TCGGGTCGGG TATTGGATTC AAGGAGGAGG ATACTCCTAA TTTCACCGGA
    TCTTTCTACT CTAAAACCAA AGCTATGGTG GAGGAGCTGC TCAAGAACTA
    TGAAAATGTA TGCACGCTAA GAGTGCGAAT GCCGATTTCA TCGGATCTAA
    CAAACCCGAG AAACTTCATC ACGAAGATTG CTCGGTATGA GAAAGTTGTG
    GACATCCCAA ACTCGATGAC AATCCTCGAT GAGCTTCTCC CGATATCAAT
    CGAAATGGCG AAGAGGAACT TAACCGGGAT CTACAATTTC ACTAACCCGG
    GTGTTGTGAG CCACAACGAG ATCTTGGAGA TGTACAGAGA CTACATTGAC
    CCGAGTTTTA CTTGGAAGAA CTTCACATTG GAGGAACAAG CTAAAGTGAT
    TGTGGCGCCA AGGAGTAACA ATGAGCTTGA TGCAACTAAG TTGAAGACTG
    AGTTCCCTGA GTTGATGTCT ATCAAAGAGT CTCTGATCAA GTTCGTGTTT
    GAGCCCAACA AGAAGACTGA AGTTAAAGCT TGAAATGTGG TTTTGTAAGG
    TAAACGGTTC TTAACCCCAA AAAAACAAAT CAAATGAAAG ACAAAAAAAG
    TTTGGGTTTT TAAAAGAAGT ATCTTTTTTT TTTTTTGGGT TTATGGGCTG
    AAAAAATTGT TGTGTTTTTT GTATTTTATT TTTTTGTTTT TCTTTTTCCC
    AATGTTGTTG TATTGTTAAA ATAAACTTTT GTTTCATTAC AAATAAGAAA
    TGTACTATTT TGCAAAAAAA AAAAAGTAAT

    Gene models with high sequence identity

    1. AT1G63000.1 [+] show detail - nucleotide-rhamnose synthase/epimerase-reductase
      E-value: 0; Score: 99.84

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam dTDP-4-dehydrorhamnose reductase 7.1e-16
    superfamily NAD(P)-binding domain 2.5e-24
    HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 3.6e-224
    HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 3.6e-224
    Gene3D NAD(P)-binding domain 1.3e-19

    See the detailed result >

    AT1G63000.1

    Model type
    Protein coding
    Short Description
    nucleotide-rhamnose synthase/epimerase-reductase
    Computational Description
    nucleotide-rhamnose synthase/epimerase-reductase (NRS/ER); FUNCTIONS IN: UDP-4-keto-rhamnose-4-keto-reductase activity, dTDP-4-dehydrorhamnose reductase activity, UDP-4-keto-6-deoxy-glucose-3,5-epimerase activity, dTDP-4-dehydrorhamnose 3,5-epimerase activity; INVOLVED IN: dTDP-rhamnose biosynthetic process, UDP-rhamnose biosynthetic process; LOCATED IN: soluble fraction, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: NAD(P)-binding domain (InterPro:IPR016040), dTDP-4-dehydrorhamnose reductase (InterPro:IPR005913); BEST Arabidopsis thaliana protein match is: rhamnose biosynthesis 1 (TAIR:AT1G78570.1); Has 1363 Blast hits to 1363 proteins in 432 species: Archae - 58; Bacteria - 688; Metazoa - 9; Fungi - 22; Plants - 263; Viruses - 5; Other Eukaryotes - 318 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR