F16937

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) increased length (PATO:0000573) (Rosette leaf::Shape::long)
	seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997) (Seed::Number::few)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others) abnormal (PATO:0000460) (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F16937

Determination of introduced cDNA(s)

RAFL05-17-E01 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-17-E01 - Resource: pda07381

full reading [+] show sequence

ACACAACCAA AGCGTTTAGA AAAAAACAGC GATAAAACCG AAACATCAAG 
CAAACAAAAA AAAAAGAGAA GAGAAATTAT TTTTTTTTGT TTTCGTTTTC 
AAAAACAAAA TCTTTGAATT TTATGGCAAC CCGTCTTCTC CGAACAAACT 
TTATCCGGCG ATCTTACCGT TTACCCGCTT TTAGCCCGGT GGGTCCTCCC 
ACCGTGACTG CTTCCACCGC CGTCGTCCCG GAGATTCTCT CCTTTGGACA 
ACAAGCACCG GAACCACCTC TTCACCACCC AAAACCCACC GAGCAATCTC 
ACGATGGTCT CGATCTCTCC GATCAAGCCC GTCTTTTCTC CTCTATCCCA 
ACCTCTGATC TCCTCCGTTC CACCGCCGTG TTGCATGCGG CGGCGATAGG 
TCCTATGGTC GACCTAGGGA CGTGGGTCAT GAGCTCTAAA CTTATGGACG 
CTTCGGTGAC GCGTGGCATG GTTTTAGGGC TTGTGAAAAG TACGTTTTAT 
GACCATTTTT GCGCCGGTGA AGATGCCGAC GCAGCCGCTG AGCGCGTGAG 
AAGCGTTTAT GAAGCTACTG GTCTTAAAGG GATGCTTGTC TATGGCGTCG 
AACACGCCGA TGACGCTGTA TCTTGTGATG ATAACATGCA ACAATTCATT 
CGAACCATTG AAGCTGCCAA ATCTTTACCA ACATCTCACT TTAGCTCAGT 
GGTTGTGAAG ATAACTGCCA TTTGTCCAAT TAGTCTTCTG AAACGAGTGA 
GCGATCTGCT GCGGTGGGAA TACAAAAGTC CGAACTTCAA ACTCTCATGG 
AAGCTCAAAT CGTTTCCGGT TTTCTCCGAA TCGAGTCCTC TCTACCACAC 
AAACTCAGAA CCGGAACCGT TAACCGCGGA AGAAGAAAGG GAGCTCGAAG 
CAGCTCATGG AAGGATTCAA GAAATCTGTA GGAAATGCCA AGAGTCCAAT 
GTACCATTGT TGATTGATGC GGAAGACACA ATCCTCCAAC CCGCGATCGA 
TTACATGGCT TATTCATCGG CGATCATGTT CAATGCTGAC AAAGACCGAC 
CAATCGTTTA CAACACGATT CAGGCGTACT TGAGAGACGC CGGTGAGAGA 
CTGCATTTGG CAGTACAAAA TGCTGAGAAA GAGAATGTTC CTATGGGGTT 
CAAGTTGGTG AGAGGGGCTT ACATGTCTAG CGAAGCTAGC TTGGCGGATT 
CCCTGGGTTG CAAGTCGCCA GTCCACGACA CAATTCAGGA TACTCACTCT 
TGTTACAATG ATTGTATGAC ATTCCTGATG GAGAAAGCAT CAAACGGTTC 
TGGTTTCGGT GTCGCTCTCG CAACACATAA CGCTGATTCG GGGAGACTTG 
CGTCGAGGAA AGCGAGTGAC CTCGGGATCG ATAAACAGAA CGGGAAGATA 
GAGTTTGCAC AGCTATATGG TATGTCAGAT GCATTGTCCT TCGGGTTAAA 
GAGAGCAGGG TTCAATGTTA GCAAGTACAT GCCGTTTGGA CCCGTCGCAA 
CCGCTATACC GTATCTTCTC CGACGCGCTT ATGAGAACCG GGGAATGATG 
GCCACCGGAG CTCATGACCG TCAACTCATG AGGATGGAAC TTAAGAGGAG 
ATTAATCGCC GGGATTGCGT AAAGAGAGAG TATGGAGCCA TTAAATGAAA 
TTGGGAAATG TAGATGAATA AATTTCTTCT ATGTAGTTTA AGAAATTGAA 
AACAAAAAAT TATAATATAA GAAATGGAGT AGGTAAGAAC ATTTCCTGTG 
GCTAAATATT TTTCATGAGG GACTATGTTT TTACTATCAA TATATCATTC 
ACAAATGTAT ATTCACCTTA TCAATAAAAA TGCTTTTTAC G

Gene models with high sequence identity

AT3G30775.1 [+] show detail - Methylenetetrahydrofolate reductase family protein
E-value: 0; Score: 99.95

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Proline dehydrogenase	1.6e-108
Gene3D	no description	6.2e-59
HMMPanther	Proline oxidase	1.6e-130
superfamily	FAD-linked oxidoreductase	2.3e-81

See the detailed result >

AT3G30775.1

Model type: Protein coding
Short Description: Methylenetetrahydrofolate reductase family protein
Curator Summary: Encodes a proline oxidase that is predicted to localize to the inner mitochondrial membrane, its mRNA expression induced by high levels of Al and by osmotic stress. The promoter contains an L-proline-inducible element.
Computational Description: EARLY RESPONSIVE TO DEHYDRATION 5 (ERD5); CONTAINS InterPro DOMAIN/s: Proline dehydrogenase (InterPro:IPR002872), Proline oxidase (InterPro:IPR015659); BEST Arabidopsis thaliana protein match is: Methylenetetrahydrofolate reductase family protein (TAIR:AT5G38710.1); Has 2269 Blast hits to 2250 proteins in 878 species: Archae - 1; Bacteria - 1355; Metazoa - 205; Fungi - 217; Plants - 85; Viruses - 2; Other Eukaryotes - 404 (source: NCBI BLink).
Link: InterPro Scan - TAIR