F22310

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F22310

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F22310

Determination of introduced cDNA(s)

  • RAFL05-07-M20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-07-M20 - Resource: pda01021

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-07-M20 - full reading
    ATCGCCGAGT CTGCTTCTTC TTCTCTCTCT CTCTCTCTAG TGGTCACACT 
    CACTTCACAC CCGTGAAAAA ACCCTAGGAA CCAAATATGG TGAACCTCTC
    GAGATCCGTC GCGTTGATCT CTGTATTCCT CCTTCCTTTA CTTAGCTTCT
    CTTTCTCCGT CGATAATCCG ACGGATCGGC GTGTTCTTGT TCTTCTCGAT
    GATTTATCAC TCAAATCCTC TCATTCTATC TTCTTCAACA CTCTCAAGTC
    TCGTGGATTC GATCTCGATT TCAAGTTAGC TGAAGATTCG AAGCTTGCGC
    TTCAACGATA CGGCCAGTAC TTGTACGATG GGTTGATCAT CTTCGCCCCA
    TCAACAGAAC GGTTTGGAGG ATCCTTGGAT TCGAAATCCA TTGCTGATTT
    TGTTGATTCC GGTCGTGACT TGATCTTATC GGCGGATACT GCTGCATCTG
    ATTTGATTAG GGGTATTGCC ACAGAGTGTG GGGTTGATTT CGATGAGGAT
    TCTTCAGCTA TGGTTATTGA TCATACTAGC TTCTCCGTCT CTGATGTTGA
    CGGCGACCAC ACCTTGATTG CTGCCGACGA TTTAGTGAAA TCCGATGTCA
    TTTTGGGAAA AACCAAGATT GAGGCTCCCG TGCTCTTCAG AGGAGTTGCA
    CATTCGTTGA ACCCTACTAA CAATCTGGTT TTGAAAGTTC TTTCAGCCTC
    TCCATCAGCT TATTCTGCTA ACCCAAGCTC GAAGTTGTCT AGTCCCCCCC
    AGCTCACTGG GTCTTCAATT TCGCTAGTCT CGGTCATGCA GGCCAGGAAC
    AATGCTAGGG TTGTGATCTC AGGCTCAGTG CAGTTGTTTA GCGATAGGTT
    AATCAGATCT GGTGTCCAGA AAGCAGGGAG TCCGAACCAA TACGAAAAAT
    CTGGCAACGA ACAGTTTGTG ACTGAACTAA GCAAATGGGT CTTCCACGAA
    AGGGGCCATC TGAAGGCTGG TAATCTTGTA CACCATAGGG TTGGAGAAAC
    AGATGAGCCA GCTATATATA GGATAAAGGA TGACCTGGAA TTTTCTGTAG
    AGATATATGA GTGGTCAGGA AAGAGCTGGG AGCCGTATGT TGCTAACGGT
    GTGCAGGTTC AGTTTTACAT GATGAGCCCG TATGTGTTGA AAACCCTGTC
    AACAGACAAG AAGGGTCTGT TTCATACATC TTTCAAGGTT CCTGATGTCT
    ACGGTGTATT CCAGTTTAAG GTTGAATATG AAAAGCTAGG CTACACTACA
    TTATCTCTTT CGAAGCAGAT TCCGGTGCGG CCTTACAGAC ACAATGAGTA
    TGAGAGATTT ATCCCAACTG CGTATCCTTA TTATGGAGCC TGCTTTACGA
    CGATGGCTGG TTTCTTCGTC TTCAGCTTCG TCTACCTCTA CCATAAGTAG
    AATCCCTTAA ATGGTGGCCA AGTCCCATAA AGTAAGCCAA ACCTCCATAT
    TTAGATGAGA AAAAACAACT TTCGAACTAG AGACCAATAT TTTTGGGCAG
    CAAGGTTTTC ATATGTTTCT AGTTTTAGCC AAACAGTTGC AACCTTCTTT
    TTGTTTCCCA GATATTTCTA TGTTAACGTA ACTTTACACC CCAAAAAAAA
    AAAAAAAA

    Gene models with high sequence identity

    1. AT5G66680.1 [+] show detail - dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit family protein
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit 8.5e-131
    HMMPanther Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit 3.4e-237

    See the detailed result >

    AT5G66680.1

    Model type
    Protein coding
    Short Description
    dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit family protein
    Curator Summary
    Encodes a protein ortholog of human SOT48 or yeast WBP1, an essential protein subunit of the oligosaccharyltransferase (OST) complex, which is responsible for the transfer in the ER of the N-linked glycan precursor onto Asn residues of candidate proteins.
    Computational Description
    DEFECTIVE GLYCOSYLATION (DGL1); FUNCTIONS IN: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; INVOLVED IN: plant-type cell wall organization, protein amino acid N-linked glycosylation via asparagine, unidimensional cell growth; LOCATED IN: in 8 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit (InterPro:IPR005013); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR