F23923

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	decreased size (PATO:0000587) (Seed::Shape::small) decreased length (PATO:0000574) (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23923

Determination of introduced cDNA(s)

RAFL09-31-A15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-31-A15 - Resource: pda05824

full reading [+] show sequence

ACCTAACAAT GACCACCGTC GTTTCCTTTC TCGCCCTCTT CCTCTTTCTA 
GTCGCCGCCG TTTCTGGTGA CGTCATCAAA CTTCCTTCTC TAGCTTCTAA 
GTTCTTCCGC CCAACTGAAA ACGACGATGA TTCTACTAAG TGGGCTGTTC 
TCGTCGCCGG ATCCAGCGGA TACTGGAATT ATCGTCATCA GGCGGATGTT 
TGTCATGCTT ATCAGCTTTT GAAGAAAGGT GGAGTGAAAG AGGAGAATAT 
TGTGGTGTTT ATGTATGATG ACATTGCGAA GAACGAGGAG AATCCAAGAC 
CTGGAGTTAT TATCAATAGT CCTAATGGAG AGGATGTCTA TAATGGAGTT 
CCCAAGGATT ACACTGGAGA TGAAGTTAAT GTTGATAACT TATTAGCTGT 
GATTCTTGGA AACAAAACGG CTCTTAAAGG AGGAAGTGGG AAAGTTGTAG 
ATAGCGGTCC AAACGATCAT ATCTTTATAT ACTATAGTGA TCACGGTGGT 
CCGGGAGTGC TCGGGATGCC AACTTCTCCA AACCTATATG CAAATGATCT 
CAATGATGTC TTGAAGAAAA AATATGCTTC AGGAACATAT AAGAGCTTGG 
TGTTTTATTT GGAGGCTTGT GAATCTGGAA GTATTTTTGA AGGTCTTTTA 
CCAGAGGGTT TAAATATTTA CGCGACAACT GCATCGAATG CAGAAGAAAG 
TAGCTGGGGT ACTTACTGTC CTGGAGAGGA TCCTAGTCCT CCTTCTGAGT 
ATGAGACCTG TTTGGGTGAC TTATACAGTG TTGCTTGGAT AGAAGATAGT 
GAAAAACACA ATTTACAAAC AGAGACTTTG CACGAGCAAT ATGAATTGGT 
GAAAAAGAGA ACTGCAGGTT CTGGTAAGTC TTATGGTTCT CATGTTATGG 
AATTTGGAGA TATAGGACTC AGCAAGGAGA AGCTTGTCCT TTTTATGGGT 
ACAAATCCAG CAGATGAAAA CTTCACCTTT GTGAATGAGA ATTCAATAAG 
GCCGCCTTCA AGAGTTACAA ACCAGCGTGA TGCGGATCTT GTCCATTTCT 
GGCATAAGTA TCAAAAGGCA CCGGAAGGGT CAGCAAGAAA AGTTGAAGCT 
CAGAAGCAAG TCCTTGAAGC AATGTCTCAC AGACTTCATG TTGATAATAG 
CATTCTGTTG ATTGGGATTC TTTTGTTTGG TTTGGAAGGT CATGCGGTGT 
TAAATAAAGT CCGGCCTTCT GGAGAACCGC TTGTTGACGA TTGGGACTGC 
CTTAAATCTC TGGTGAGAGC TTTCGAGAGG CACTGTGGAT CGTTGTCTCA 
GTACGGAATA AAGCACATGA GGTCGATTGC AAACATGTGC AACGCAGGGA 
TTCAGATGAG GCAAATGGAG GAGGCAGCAA TGCAGGCTTG TCCCACCATC 
CCTACCAGTC CTTGGAGCTC TCTTGACCGT GGATTCAGTG CTTGATCTTC 
GTCTCCGCCC TATATTAATC GATGTCCATT AATATCTTAC TACCAATTAT 
ACTCCTTCCT ATGTAGACAA GATGTTGTAA ATATGGGATG CTTAAGTTCT 
TGATGTCACA GACAAAAGAA ACAGTGATTG TAAGTAGTTG TTTCAAATAA 
TCTTTGTTGT TCAAGT

Gene models with high sequence identity

AT2G25940.1 [+] show detail - alpha-vacuolar processing enzyme
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMPanther	VACUOLAR PROCESSING ENZYME-RELATED	0
HMMPanther	Peptidase C13, legumain	0
HMMPfam	Peptidase C13, legumain	7.5e-113
FPrintScan	Peptidase C13, legumain	6.6e-57
FPrintScan	Peptidase C13, legumain	6.6e-57
FPrintScan	Peptidase C13, legumain	6.6e-57
FPrintScan	Peptidase C13, legumain	6.6e-57
FPrintScan	Peptidase C13, legumain	6.6e-57

See the detailed result >

AT2G25940.1

Model type: Protein coding
Short Description: alpha-vacuolar processing enzyme
Curator Summary: Encodes a vacuolar processing enzyme belonging to a novel group of cysteine proteinases that is expressed in vegetative organs and is upregulated in association with various types of cell death and under stressed conditions.
Computational Description: alpha-vacuolar processing enzyme (ALPHA-VPE); FUNCTIONS IN: cysteine-type endopeptidase activity; INVOLVED IN: proteolysis, vacuolar protein processing; LOCATED IN: endomembrane system; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Peptidase C13, legumain (InterPro:IPR001096); BEST Arabidopsis thaliana protein match is: gamma vacuolar processing enzyme (TAIR:AT4G32940.1); Has 793 Blast hits to 791 proteins in 239 species: Archae - 4; Bacteria - 12; Metazoa - 278; Fungi - 115; Plants - 259; Viruses - 0; Other Eukaryotes - 125 (source: NCBI BLink).
Link: InterPro Scan - TAIR