F23923

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23923

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23923

Determination of introduced cDNA(s)

  • RAFL09-31-A15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-31-A15 - Resource: pda05824

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-31-A15 - full reading
    ACCTAACAAT GACCACCGTC GTTTCCTTTC TCGCCCTCTT CCTCTTTCTA 
    GTCGCCGCCG TTTCTGGTGA CGTCATCAAA CTTCCTTCTC TAGCTTCTAA
    GTTCTTCCGC CCAACTGAAA ACGACGATGA TTCTACTAAG TGGGCTGTTC
    TCGTCGCCGG ATCCAGCGGA TACTGGAATT ATCGTCATCA GGCGGATGTT
    TGTCATGCTT ATCAGCTTTT GAAGAAAGGT GGAGTGAAAG AGGAGAATAT
    TGTGGTGTTT ATGTATGATG ACATTGCGAA GAACGAGGAG AATCCAAGAC
    CTGGAGTTAT TATCAATAGT CCTAATGGAG AGGATGTCTA TAATGGAGTT
    CCCAAGGATT ACACTGGAGA TGAAGTTAAT GTTGATAACT TATTAGCTGT
    GATTCTTGGA AACAAAACGG CTCTTAAAGG AGGAAGTGGG AAAGTTGTAG
    ATAGCGGTCC AAACGATCAT ATCTTTATAT ACTATAGTGA TCACGGTGGT
    CCGGGAGTGC TCGGGATGCC AACTTCTCCA AACCTATATG CAAATGATCT
    CAATGATGTC TTGAAGAAAA AATATGCTTC AGGAACATAT AAGAGCTTGG
    TGTTTTATTT GGAGGCTTGT GAATCTGGAA GTATTTTTGA AGGTCTTTTA
    CCAGAGGGTT TAAATATTTA CGCGACAACT GCATCGAATG CAGAAGAAAG
    TAGCTGGGGT ACTTACTGTC CTGGAGAGGA TCCTAGTCCT CCTTCTGAGT
    ATGAGACCTG TTTGGGTGAC TTATACAGTG TTGCTTGGAT AGAAGATAGT
    GAAAAACACA ATTTACAAAC AGAGACTTTG CACGAGCAAT ATGAATTGGT
    GAAAAAGAGA ACTGCAGGTT CTGGTAAGTC TTATGGTTCT CATGTTATGG
    AATTTGGAGA TATAGGACTC AGCAAGGAGA AGCTTGTCCT TTTTATGGGT
    ACAAATCCAG CAGATGAAAA CTTCACCTTT GTGAATGAGA ATTCAATAAG
    GCCGCCTTCA AGAGTTACAA ACCAGCGTGA TGCGGATCTT GTCCATTTCT
    GGCATAAGTA TCAAAAGGCA CCGGAAGGGT CAGCAAGAAA AGTTGAAGCT
    CAGAAGCAAG TCCTTGAAGC AATGTCTCAC AGACTTCATG TTGATAATAG
    CATTCTGTTG ATTGGGATTC TTTTGTTTGG TTTGGAAGGT CATGCGGTGT
    TAAATAAAGT CCGGCCTTCT GGAGAACCGC TTGTTGACGA TTGGGACTGC
    CTTAAATCTC TGGTGAGAGC TTTCGAGAGG CACTGTGGAT CGTTGTCTCA
    GTACGGAATA AAGCACATGA GGTCGATTGC AAACATGTGC AACGCAGGGA
    TTCAGATGAG GCAAATGGAG GAGGCAGCAA TGCAGGCTTG TCCCACCATC
    CCTACCAGTC CTTGGAGCTC TCTTGACCGT GGATTCAGTG CTTGATCTTC
    GTCTCCGCCC TATATTAATC GATGTCCATT AATATCTTAC TACCAATTAT
    ACTCCTTCCT ATGTAGACAA GATGTTGTAA ATATGGGATG CTTAAGTTCT
    TGATGTCACA GACAAAAGAA ACAGTGATTG TAAGTAGTTG TTTCAAATAA
    TCTTTGTTGT TCAAGT

    Gene models with high sequence identity

    1. AT2G25940.1 [+] show detail - alpha-vacuolar processing enzyme
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther VACUOLAR PROCESSING ENZYME-RELATED 0
    HMMPanther Peptidase C13, legumain 0
    HMMPfam Peptidase C13, legumain 7.5e-113
    FPrintScan Peptidase C13, legumain 6.6e-57
    FPrintScan Peptidase C13, legumain 6.6e-57
    FPrintScan Peptidase C13, legumain 6.6e-57
    FPrintScan Peptidase C13, legumain 6.6e-57
    FPrintScan Peptidase C13, legumain 6.6e-57

    See the detailed result >

    AT2G25940.1

    Model type
    Protein coding
    Short Description
    alpha-vacuolar processing enzyme
    Curator Summary
    Encodes a vacuolar processing enzyme belonging to a novel group of cysteine proteinases that is expressed in vegetative organs and is upregulated in association with various types of cell death and under stressed conditions.
    Computational Description
    alpha-vacuolar processing enzyme (ALPHA-VPE); FUNCTIONS IN: cysteine-type endopeptidase activity; INVOLVED IN: proteolysis, vacuolar protein processing; LOCATED IN: endomembrane system; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Peptidase C13, legumain (InterPro:IPR001096); BEST Arabidopsis thaliana protein match is: gamma vacuolar processing enzyme (TAIR:AT4G32940.1); Has 793 Blast hits to 791 proteins in 239 species: Archae - 4; Bacteria - 12; Metazoa - 278; Fungi - 115; Plants - 259; Viruses - 0; Other Eukaryotes - 125 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR