F24229

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F24229

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F24229

Determination of introduced cDNA(s)

  • RAFL09-46-J03 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-46-J03 - Resource: pda06285

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-46-J03 - full reading 
    CCTTCCATGG CCTTCTTCTC CATGATCTCC ATTCTCCTTG GCTTTGTTAT 
    CTCCTCCTTC ATCTTCATCT TCTTCTTCAA GAAACTTCTC TCCTTCTCCA 
    GAAAGAACAT GTCTGAAGTC TCCACTCTCC CCTCTGTTCC AGTGGTACCA 
    GGGTTTCCTG TTATTGGGAA CTTGCTGCAA CTAAAAGAGA AGAAACCTCA 
    CAAGACTTTC ACTAGATGGT CAGAGATTTA TGGTCCTATT TACTCTATAA 
    AGATGGGTTC TTCTTCTCTT ATTGTCCTCA ATTCTACTGA GACTGCCAAA 
    GAGGCCATGG TGACGCGGTT TTCGTCTATC TCAACGAGGA AGTTGTCAAA 
    TGCGTTGACA GTCCTTACTT GTGACAAATC TATGGTTGCT ACTAGTGATT 
    ATGATGATTT CCACAAGTTG GTGAAACGGT GTCTCTTGAA CGGTCTTTTG 
    GGTGCTAATG CACAGAAACG AAAAAGACAT TACAGAGATG CACTCATTGA 
    AAATGTGTCT TCCAAGTTGC ATGCCCATGC TAGGGACCAT CCACAAGAAC 
    CTGTAAACTT CAGAGCTATA TTTGAGCATG AGCTTTTCGG TGTAGCATTG 
    AAGCAAGCTT TTGGGAAAGA TGTGGAATCC ATTTATGTTA AAGAACTCGG 
    TGTGACTTTG TCGAAAGACG AGATCTTCAA GGTTTTAGTA CATGACATGA 
    TGGAAGGTGC AATTGATGTT GATTGGAGAG ACTTCTTCCC ATACTTGAAA 
    TGGATTCCAA ATAAAAGTTT TGAAGCAAGA ATCCAGCAAA AGCATAAACG 
    TAGACTCGCA GTGATGAATG CTCTGATTCA AGATCGACTG AAGCAGATGG 
    TTCAGAATCG GATGATGATT GCTATCTCAA CTTCTTGATG TCGGAAGCGA 
    AAACACTAAC CAAGGAGCAA ATTGCTATCT TGGTTTGGGA GACGATTATC 
    GAGACAGCTG ACACTACTTT GGTTACAACT GAATGGGCCA TCTATGAGCT 
    CGCTAAGCAT CCAAGTGTCC AAGATCGTCT GTGCAAAGAA ATCCAAAATG 
    TCTGCGGAGG AGAAAAGTTC AAAGAAGAGC AATTGTCTCA AGTTCCTTAT 
    CTCAATGGAG TCTTTCATGA AACGCTTAGG AAATACAGTC CTGCTCCTCT 
    AGTTCCCATT CGCTACGCCC ACGAGGATAC GCAAATCGGA GGCTATCATG 
    TCCCTGCAGG AAGTGAGATT GCAATAAACA TATATGGATG CAACATGGAT 
    AAGAAGCGTT GGGAGAGACC AGAGGACTGG TGGCCGGAGC GGTTTCTTGA 
    TGATGGCAAA TATGAAACGT CAGATCTTCA CAAGACAATG GCGTTTGGAG 
    CGGGAAAGAG GGTTTGTGCT GGTGCTCTTC AAGCATCTCT CATGGCAGGC 
    ATTGCTATTG GAAGATTAGT GCAAGAATTC GAGTGGAAGC TTAGAGATGG 
    CGAAGAAGAG AATGTGGATA CATATGGCTT GACCTCTCAG AAGCTTTATC 
    CTCTTATGGC TATTATCAAT CCAAGGCGTT CTTAAGACAG AAATCTCTTC 
    TTTCTTTTGT TAGTTTTTAA CCTTGTATCA ACTTTTATTT GTAACAAATG 
    CTGTTTTGTA GTACTATTTA TGTTTTACTG TGTTATATTT TACC

    Gene models with high sequence identity

    1. AT5G25900.1 [+] show detail - GA requiring 3
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Cytochrome P450 7.5e-25
    Gene3D Cytochrome P450 3.7e-58
    superfamily Cytochrome P450 1.7e-30
    superfamily Cytochrome P450 2.4e-65
    PatternScan Cytochrome P450, conserved site 0
    HMMPanther gb def: Ent-kaurene oxidase (EC 1.14.13.78) ( 8.8e-177
    HMMPanther Cytochrome P450 8.8e-177
    HMMPanther gb def: Ent-kaurene oxidase (EC 1.14.13.78) ( 3.2e-194
    HMMPanther Cytochrome P450 3.2e-194
    FPrintScan Cytochrome P450, E-class, group I 2.2e-05
    FPrintScan Cytochrome P450, E-class, group I 2.2e-05
    FPrintScan Cytochrome P450, E-class, group I 2.5e-21
    FPrintScan Cytochrome P450, E-class, group I 2.5e-21
    FPrintScan Cytochrome P450, E-class, group I 2.5e-21
    FPrintScan Cytochrome P450, E-class, group I 2.5e-21
    FPrintScan Cytochrome P450, E-class, group I 2.5e-21
    FPrintScan Cytochrome P450, C-terminal 2.5e-05
    FPrintScan Cytochrome P450, C-terminal 2.5e-05
    FPrintScan Cytochrome P450, C-terminal 2.5e-05
    HMMPfam Cytochrome P450 1.6e-17
    HMMPfam Cytochrome P450 1.9e-56

    See the detailed result >

    AT5G25900.1

    Model type
    Protein coding
    Short Description
    GA requiring 3
    Curator Summary
    Encodes a member of the CYP701A cytochrome p450 family that is involved in later steps of the gibberellin biosynthetic pathway.
    Computational Description
    GA requiring 3 (GA3); FUNCTIONS IN: oxygen binding; INVOLVED IN: gibberellin biosynthetic process, gibberellic acid mediated signaling pathway, ent-kaurene oxidation to kaurenoic acid; LOCATED IN: chloroplast outer membrane, endoplasmic reticulum, microsome; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT5G07990.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR