F25350

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F25350

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F25350

Determination of introduced cDNA(s)

  • RAFL05-04-N11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-04-N11 - Resource: pda02449

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-04-N11 - full reading
    AAAACTCTCC GACGATGCCT CAGGCACCGA TGCCAGAGTT CTCTAGCTCG 
    GTGAAGCTCA AGTACGTGAA ACTTGGTTAC CAATATTTGG TTAACCATTT
    CTTGAGTTTT CTTTTGATCC CGATCATGGC TATTGTCGCC GTTGAGCTTC
    TTCGGATGGG TCCTGAAGAG ATCCTTAATG TTTGGAATTC ACTCCAGTTT
    GACCTAGTTC AGGTTCTATG TTCTTCCTTC TTAGTCATCT TCATCTCCAC
    TGTTTACTTC ATGTCCAAGC CACGCACCAT CTACCTCGTT GACTATTCTT
    GTTACAAGCC ACCTGTCACG TGTCGTGTCC CCTTCGCAAC TTTCATGGAA
    CACTCTCGTT TGATCCTCAA GGACAAGCCT AAGAGCGTCG AGTTCCAAAT
    GAGAATCCTT GAACGTTCTG GCCTCGGTGA GGAGACTTGT CTCCCTCCGG
    CTATTCATTA TATTCCTCCC ACACCAACCA TGGACGCGGC TAGAAGCGAG
    GCTCAGATGG TTATCTTCGA GGCCATGGAC GATCTTTTCA AGAAAACCGG
    TCTTAAACCT AAAGACGTCG ACATCCTTAT CGTCAACTGC TCTCTTTTCT
    CTCCCACACC ATCGCTCTCA GCTATGGTCA TCAACAAATA TAAGCTTAGG
    AGTAATATCA AGAGCTTCAA TCTTTCGGGG ATGGGCTGCA GCGCGGGCCT
    GATCTCAGTT GATCTAGCCC GCGACTTGCT CCAAGTTCAT CCCAATTCAA
    ATGCAATCAT CGTCAGCACG GAGATCATAA CGCCTAATTA CTATCAAGGC
    AACGAGAGAG CCATGTTGTT ACCCAATTGT CTCTTCCGCA TGGGTGCGGC
    AGCCATACAC ATGTCAAACC GCCGGTCTGA CCGGTGGCGA GCCAAATACA
    AGCTTTCCCA CCTCGTCCGG ACACACCGTG GCGCTGACGA CAAGTCTTTC
    TACTGTGTCT ACGAACAGGA AGACAAAGAA GGACACGTTG GCATCAACTT
    GTCCAAAGAT CTCATGGCCA TCGCCGGTGA AGCCCTCAAG GCAAACATCA
    CCACAATAGG TCCTTTGGTC CTACCGGCGT CAGAACAACT TCTCTTCCTC
    ACGTCCCTAA TCGGACGTAA AATCTTCAAC CCGAAATGGA AACCATACAT
    ACCGGATTTC AAGCTGGCCT TCGAACACTT TTGCATTCAC GCAGGAGGCA
    GAGCGGTGAT CGACGAGCTC CAAAAGAATC TACAACTATC AGGAGAACAC
    GTTGAGGCCT CAAGAATGAC ACTACATCGT TTTGGTAACA CGTCATCTTC
    ATCGTTATGG TACGAGCTTA GCTACATCGA GTCTAAAGGG AGAATGAGGA
    GAGGCGATCG CGTTTGGCAA ATCGCGTTTG GGAGTGGTTT CAAGTGTAAC
    TCTGCCGTGT GGAAATGTAA CCGTACGATT AAGACACCTA AGGACGGACC
    ATGGTCCGAT TGTATCGACC GTTACCCTGT CTTTATTCCC GAAGTTGTCA
    AACTCTAAAC TGAAAACGTC TTTGAACGGT TTAGTAACGG TTTGATTTTG
    TGTTACGGTT TAGGTTTATT TGGTCTCGGG ATTTGGTTTA AAGGGGATTG
    AGAAATGGGA AGTTAGAGAG AAGAAAAAGC AAAGCATAAA TGTTTGTATT
    TAATTGCTCT GCATATACTT AAATCTCTGC TTTTCATTTG GGGTATTTTT
    TAGTTTCTCG TGCTGTAATT AATAACTTGT GGTGTACTCA AATAAGAATA
    TTTCTCTCTG TTTTATTATT TTTAAATTGG AGAATAAACT TCTTTGTTTT
    AAAAAAAAAA AAAAT

    Gene models with high sequence identity

    1. AT1G68530.1 [+] show detail - 3-ketoacyl-CoA synthase 6
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam FAE1/Type III polyketide synthase-like protein 4.8e-137
    HMMPfam 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal 2.9e-12
    HMMPIR Very-long-chain 3-ketoacyl-CoA synthase 0
    Gene3D Thiolase-like, subgroup 3.3e-54
    Gene3D Thiolase-like, subgroup 3.5e-24
    superfamily Thiolase-like 2.4e-94

    See the detailed result >

    AT1G68530.1

    Model type
    Protein coding
    Short Description
    3-ketoacyl-CoA synthase 6
    Curator Summary
    Encodes KCS6, a member of the 3-ketoacyl-CoA synthase family involved in the biosynthesis of VLCFA (very long chain fatty acids).
    Computational Description
    3-ketoacyl-CoA synthase 6 (KCS6); FUNCTIONS IN: transferase activity, transferring acyl groups other than amino-acyl groups, catalytic activity; INVOLVED IN: in 7 processes; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase-like (InterPro:IPR016039), Very-long-chain 3-ketoacyl-CoA synthase (InterPro:IPR012392), 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (InterPro:IPR013747), FAE1/Type III polyketide synthase-like protein (InterPro:IPR013601), Thiolase-like, subgroup (InterPro:IPR016038); BEST Arabidopsis thaliana protein match is: 3-ketoacyl-CoA synthase 5 (TAIR:AT1G25450.1); Has 4523 Blast hits to 4503 proteins in 1079 species: Archae - 0; Bacteria - 1483; Metazoa - 0; Fungi - 25; Plants - 2866; Viruses - 0; Other Eukaryotes - 149 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR