F27742

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27742

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27742

Determination of introduced cDNA(s)

  • RAFL09-63-I19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-63-I19 - Resource: pda17794

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-63-I19 - full reading 
    CCCCGATGGA TCCATTTTGA CGTCATCTTT ATCACTATCT CTCTATTATA 
    TAAATCTCTT CGTACCCTTT TACCGATTCT TCACCGTGAT CGCTTAATCA 
    GACCTCAATT TCGTTGTTAA AGAACAAAGC TTTAAGCAGC CATGGATCCA 
    AACCAACGTA TCGCGAGAAT CTCTGCTCAT CTCAATCCTC CTAATCTTCA 
    TAATCAGATT GCTGACGGGT CAGGTTTGAA TCGGGTGGCT TGTCGGGCAA 
    AAGGTGGATC ACCCGGATTC AAAGTGGCGA TACTTGGAGC AGCTGGTGGA 
    ATTGGACAAC CTCTTGCGAT GTTGATGAAG ATGAATCCTT TGGTTTCGGT 
    TCTTCATCTC TATGATGTTG CTAATGCTCC TGGTGTTACT GCTGATATTA 
    GTCATATGGA TACTAGTGCC GTTGTTCGTG GATTTCTCGG GCAGCCGCAG 
    TTAGAGGAAG CACTTACGGG TATGGATTTA GTGATCATAC CTGCTGGTGT 
    TCCGAGGAAA CCAGGGATGA CGAGGGATGA TCTGTTTAAC ATTAATGCTG 
    GGATTGTGAG GACACTCTCT GAAGCTATAG CTAAATGTTG TCCTAAAGCA 
    ATTGTGAATA TAATCAGTAA TCCGGTGAAC TCCACGGTGC CAATCGCAGC 
    TGAGGTTTTC AAGAAAGCTG GAACCTTTGA TCCAAAGAAA CTCATGGGTG 
    TCACTATGCT TGATGTTGTT AGAGCTAATA CCTTTGTGGC GGAAGTAATG 
    AGTCTTGATC CCCGTGAAGT TGAAGTTCCG GTTGTTGGAG GACACGCAGG 
    AGTTACGATT TTACCACTGC TTTCGCAGGT GAAACCTCCT TGCTCGTTCA 
    CTCAAAAAGA GATTGAATAT CTCACAGACC GCATCCAAAA CGGTGGCACT 
    GAAGTTGTTG AGGCTAAAGC TGGAGCAGGT TCTGCAACAC TATCCATGGC 
    ATATGCAGCA GTGGAGTTTG CAGATGCTTG CCTCAGGGGT CTACGAGGTG 
    ATGCAAACAT CGTTGAGTGC GCATATGTGG CATCCCATGT GACTGAGCTT 
    CCCTTCTTCG CATCGAAGGT GCGTCTGGGA CGATGTGGGA TCGATGAAGT 
    GTACGGCCTT GGACCATTGA ACGAATATGA GAGGATGGGA TTAGAGAAGG 
    CAAAGAAAGA GCTTTCAGTA AGTATTCATA AAGGTGTTAC CTTTGCGAAG 
    AAATAAAGAG ACTCGATCGT GAATAAACAC ACTTAAGCGA TGGTTTTGGA 
    ATAGTCAGAG TTTTGGAATA AGAATAATGC CTCACAATAA AAGCTCTTGC 
    GGTCTTCTTG GATCCAATCT TAAAGGTTCA AGAAACTCAT CTCCTTTAGG 
    TAAAATCTTC GATTGTTTTA TCGTTCCATC GAACCACTTT GTTCTTAGAT 
    ACAAGAACGT TTATGATTTA TGTAGTTGGG CTATAAAAGT GAGAACAGAG 
    CAATAATCTT GCAACATTTT TTCTCATCTT CTTGGTGTGT TTTTTTTTTT 
    GTTGGTTTTC ATCTTTTTGT TCTTGCTCAT GAGAGCATCT TTAGAAGGCT 
    ATTGTTGGGA AGTAAATAAG TTTGCATCGC GGAAAAGATG ATCAAGGTCA 
    TTCGGGATAA AAAAAA

    Gene models with high sequence identity

    1. AT2G22780.1 [+] show detail - peroxisomal NAD-malate dehydrogenase 1
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Malate dehydrogenase, active site 0
    superfamily Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal 1.7e-51
    superfamily NAD(P)-binding domain 6.1e-42
    HMMPIR L-lactate/malate dehydrogenase 2.1e-49
    Gene3D NAD(P)-binding domain 1.5e-42
    Gene3D Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal 2e-57
    HMMTigr Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria 2.3e-172
    HMMPanther Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria 2.2e-195
    HMMPanther MALATE AND LACTATE DEHYDROGENASE 2.2e-195
    HMMPfam Lactate/malate dehydrogenase, N-terminal 1e-48
    HMMPfam Lactate/malate dehydrogenase, C-terminal 9.9e-46

    See the detailed result >

    AT2G22780.1

    Model type
    Protein coding
    Short Description
    peroxisomal NAD-malate dehydrogenase 1
    Curator Summary
    encodes an peroxisomal NAD-malate dehydrogenase that is involved in fatty acid beta-oxidation through providing NAD to the process of converting fatty acyl CoA to acetyl CoA.
    Computational Description
    peroxisomal NAD-malate dehydrogenase 1 (PMDH1); FUNCTIONS IN: in 6 functions; INVOLVED IN: regulation of fatty acid beta-oxidation, regulation of photorespiration; LOCATED IN: chloroplast, peroxisome; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Lactate/malate dehydrogenase, N-terminal (InterPro:IPR001236), Lactate/malate dehydrogenase, C-terminal (InterPro:IPR022383), NAD(P)-binding domain (InterPro:IPR016040), Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria (InterPro:IPR010097), L-lactate/malate dehydrogenase (InterPro:IPR001557), Malate dehydrogenase, active site (InterPro:IPR001252), Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal (InterPro:IPR015955); BEST Arabidopsis thaliana protein match is: peroxisomal NAD-malate dehydrogenase 2 (TAIR:AT5G09660.1); Has 16991 Blast hits to 16989 proteins in 5457 species: Archae - 237; Bacteria - 11827; Metazoa - 1259; Fungi - 489; Plants - 758; Viruses - 0; Other Eukaryotes - 2421 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR