F28650

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28650

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • upturned (PATO:0002031)
    (Rosette leaf::Shape::hyponastic)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28650

Determination of introduced cDNA(s)

  • RAFL05-16-L13 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-16-L13 - Resource: pda01306

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-16-L13 - full reading
    AATGATTCCT TCGTACGCAT CCTCATCTTC TTCTTCTTCT CTCTTCTACT 
    GTATTTAAAC ACTCTCTCGT CTTCTCCTTG GATCCAGAGA GAGACACAAC
    ACACCATCTC AATCATGTCT TCTTCGTTTC TCTCCTCCAC TGCTTTCTTC
    CTCCTCCTTT GTCTAGGATT CTGCCACGTG TCCTCTTCCT CCTCCGACCA
    AGGAACTTAC ATCGTTCACA TGGCTAAATC TCAGATGCCG TCGTCTTTCG
    ACCTCCACTC TAACTGGTAC GATTCATCTC TCAGATCTAT CTCCGACTCA
    GCCGAATTGC TCTACACTTA CGAGAACGCG ATTCATGGAT TCTCAACACG
    GCTAACTCAA GAAGAAGCCG ACTCGCTCAT GACTCAACCT GGTGTTATCT
    CCGTTTTACC GGAGCACCGT TACGAGCTAC ACACCACTCG TACTCCTCTC
    TTCCTCGGTC TCGACGAACA CACCGCAGAT CTGTTCCCTG AAGCCGGCTC
    TTACAGCGAC GTCGTCGTCG GAGTTCTCGA TACCGGAGTT TGGCCCGAGA
    GTAAAAGCTA CTCCGACGAA GGATTCGGTC CTATTCCTTC CTCCTGGAAA
    GGCGGATGCG AGGCCGGAAC CAATTTCACC GCTTCTCTCT GTAACCGTAA
    ACTAATCGGA GCAAGATTCT TCGCTCGTGG TTACGAATCA ACGATGGGAC
    CAATCGATGA ATCTAAAGAA TCAAGATCTC CTAGAGACGA CGACGGTCAC
    GGAACTCACA CCTCATCGAC CGCCGCTGGA TCCGTCGTTG AAGGAGCTAG
    CTTATTAGGC TACGCTTCAG GAACAGCTCG TGGTATGGCT CCACGCGCTC
    GTGTCGCTGT TTACAAAGTC TGTTGGCTCG GTGGTTGTTT CAGCTCAGAT
    ATTTTAGCTG CAATCGATAA AGCCATCGCC GATAACGTCA ATGTCCTGTC
    GATGTCACTC GGCGGTGGAA TGTCGGATTA TTATAGAGAC GGTGTTGCGA
    TCGGAGCATT CGCCGCCATG GAAAGAGGGA TTTTAGTATC TTGCTCAGCT
    GGTAATGCTG GTCCGAGTAG TTCTAGTTTA TCAAACGTAG CTCCATGGAT
    CACAACTGTT GGTGCTGGTA CTTTAGATCG TGATTTTCCG GCGCTTGCGA
    TTCTCGGTAA CGGCAAGAAT TTCACCGGAG TTTCTTTGTT TAAAGGAGAA
    GCTTTACCGG ATAAATTGTT GCCGTTTATT TACGCTGGGA ATGCTAGTAA
    TGCTACTAAT GGTAATCTCT GTATGACCGG AACTTTGATC CCGGAGAAAG
    TAAAGGGGAA GATTGTGATG TGTGATAGAG GAATTAATGC TAGAGTTCAG
    AAAGGTGATG TGGTTAAAGC AGCAGGTGGA GTTGGAATGA TTCTGGCTAA
    CACTGCGGCG AATGGTGAAG AGCTTGTTGC GGATGCTCAT TTGTTACCGG
    CGACCACCGT TGGTGAAAAA GCCGGTGATA TAATCCGGCA TTACGTGACT
    ACTGATCCTA ATCCCACCGC TTCGATTTCA ATCTTAGGAA CAGTCGTCGG
    TGTTAAACCA TCTCCGGTTG TCGCAGCGTT TAGCTCACGT GGACCGAATT
    CGATTACACC GAATATTCTT AAACCGGATC TGATCGCTCC TGGAGTAAAC
    ATCCTCGCCG CGTGGACCGG TGCTGCTGGA CCAACCGGAC TCGCTTCCGA
    TTCTCGCCGC GTGGAGTTCA ATATCATCTC TGGGACGTCG ATGTCTTGCC
    CTCACGTTAG TGGTTTAGCG GCGCTTCTCA AGTCGGTGCA TCCTGAATGG
    AGCCCGGCGG CGATTAGATC GGCGCTTATG ACCACCGCTT ACAAAACCTA
    CAAAGACGGT AAACCGTTAC TCGACATCGC GACAGGGAAG CCTTCGACGC
    CGTTCGATCA CGGTGCAGGA CACGTGTCAC CAACAACTGC CACTAATCCA
    GGACTCATCT ACGATCTAAC GACGGAGGAT TACTTAGGCT TCCTCTGCGC
    ATTGAATTAC ACATCGCCGC AAATTCGAAG TGTCTCGAGA CGTAATTACA
    CTTGCGATCC GAGTAAATCG TACTCGGTCG CTGATTTGAA CTACCCGTCG
    TTCGCCGTTA ACGTTGATGG AGTCGGTGCG TATAAGTACA CGCGCACGGT
    GACGAGCGTG GGAGGAGCTG GGACTTACTC GGTTAAAGTA ACTTCGGAGA
    CGACAGGAGT CAAGATTTCG GTTGAACCGG CGGTTTTGAA TTTCAAGGAA
    GCTAACGAGA AAAAATCGTA TACGGTGACG TTTACTGTAG ACTCGTCGAA
    GCCGTCTGGA TCTAACAGCT TTGGGAGTAT TGAATGGTCG GATGGGAAAC
    ACGTGGTGGG AAGTCCCGTG GCGATTAGCT GGACATAGTC GTAAATTATG
    AGAAATGAAA GGGGTTTTAT GGAAAAGAAA AGGTGTTTAC AGATTTATTG
    TAATGCCGTT TTTAGATACG AAGAAACCTC TGTTTAGCAA AAACGACATG
    CAGTCTGTTA AGACTTGCCT AGTTTGTTTT TGGGATGTCT TGCTGTTTTT
    AATTTTAAAA AGAAATATAT TCTCCGGCTA CAACAGTAGC TGGGTCTCTC
    TCAAAAAAAA AAAAAAAA

    Gene models with high sequence identity

    1. AT5G67360.1 [+] show detail - Subtilase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Peptidase S8/S53, subtilisin/kexin/sedolisin 9.1e-80
    superfamily Transferrin receptor ectodomain, apical domai 9.2e-08
    superfamily Proteinase inhibitor, propeptide 2.9e-07
    HMMPanther SUBTILISIN-LIKE PROTEASE (PLANT) 0
    HMMPanther Peptidase S8, subtilisin-related 0
    Gene3D Peptidase S8/S53, subtilisin/kexin/sedolisin 6.4e-72
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 2.7e-13
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 2.7e-13
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 2.7e-13
    HMMPfam Peptidase S8/S53, subtilisin/kexin/sedolisin 3.2e-30
    HMMPfam Proteinase inhibitor I9, subtilisin propeptide 1.4e-17
    HMMPfam Protease-associated PA 8.9e-13
    PatternScan Peptidase S8/S53, subtilisin, active site 0

    See the detailed result >

    AT5G67360.1

    Model type
    Protein coding
    Short Description
    Subtilase family protein
    Curator Summary
    Encodes a subtilisin-like serine protease essential for mucilage release from seed coats.
    Computational Description
    ARA12; FUNCTIONS IN: serine-type endopeptidase activity; INVOLVED IN: proteolysis, seed coat development, mucilage metabolic process involved seed coat development, mucilage extrusion from seed coat; LOCATED IN: extracellular region, apoplast, cell wall, plant-type cell wall; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 17 growth stages; CONTAINS InterPro DOMAIN/s: Protease-associated PA (InterPro:IPR003137), Proteinase inhibitor, propeptide (InterPro:IPR009020), Peptidase S8/S53, subtilisin/kexin/sedolisin (InterPro:IPR000209), Peptidase S8, subtilisin-related (InterPro:IPR015500), Peptidase S8/S53, subtilisin, active site (InterPro:IPR022398), Proteinase inhibitor I9, subtilisin propeptide (InterPro:IPR010259); BEST Arabidopsis thaliana protein match is: Subtilase family protein (TAIR:AT2G05920.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR