F28650

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark) upturned (PATO:0002031) (Rosette leaf::Shape::hyponastic) abnormal (PATO:0000460) (Rosette leaf::Others) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28650

Determination of introduced cDNA(s)

RAFL05-16-L13 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-16-L13 - Resource: pda01306

full reading [+] show sequence

AATGATTCCT TCGTACGCAT CCTCATCTTC TTCTTCTTCT CTCTTCTACT 
GTATTTAAAC ACTCTCTCGT CTTCTCCTTG GATCCAGAGA GAGACACAAC 
ACACCATCTC AATCATGTCT TCTTCGTTTC TCTCCTCCAC TGCTTTCTTC 
CTCCTCCTTT GTCTAGGATT CTGCCACGTG TCCTCTTCCT CCTCCGACCA 
AGGAACTTAC ATCGTTCACA TGGCTAAATC TCAGATGCCG TCGTCTTTCG 
ACCTCCACTC TAACTGGTAC GATTCATCTC TCAGATCTAT CTCCGACTCA 
GCCGAATTGC TCTACACTTA CGAGAACGCG ATTCATGGAT TCTCAACACG 
GCTAACTCAA GAAGAAGCCG ACTCGCTCAT GACTCAACCT GGTGTTATCT 
CCGTTTTACC GGAGCACCGT TACGAGCTAC ACACCACTCG TACTCCTCTC 
TTCCTCGGTC TCGACGAACA CACCGCAGAT CTGTTCCCTG AAGCCGGCTC 
TTACAGCGAC GTCGTCGTCG GAGTTCTCGA TACCGGAGTT TGGCCCGAGA 
GTAAAAGCTA CTCCGACGAA GGATTCGGTC CTATTCCTTC CTCCTGGAAA 
GGCGGATGCG AGGCCGGAAC CAATTTCACC GCTTCTCTCT GTAACCGTAA 
ACTAATCGGA GCAAGATTCT TCGCTCGTGG TTACGAATCA ACGATGGGAC 
CAATCGATGA ATCTAAAGAA TCAAGATCTC CTAGAGACGA CGACGGTCAC 
GGAACTCACA CCTCATCGAC CGCCGCTGGA TCCGTCGTTG AAGGAGCTAG 
CTTATTAGGC TACGCTTCAG GAACAGCTCG TGGTATGGCT CCACGCGCTC 
GTGTCGCTGT TTACAAAGTC TGTTGGCTCG GTGGTTGTTT CAGCTCAGAT 
ATTTTAGCTG CAATCGATAA AGCCATCGCC GATAACGTCA ATGTCCTGTC 
GATGTCACTC GGCGGTGGAA TGTCGGATTA TTATAGAGAC GGTGTTGCGA 
TCGGAGCATT CGCCGCCATG GAAAGAGGGA TTTTAGTATC TTGCTCAGCT 
GGTAATGCTG GTCCGAGTAG TTCTAGTTTA TCAAACGTAG CTCCATGGAT 
CACAACTGTT GGTGCTGGTA CTTTAGATCG TGATTTTCCG GCGCTTGCGA 
TTCTCGGTAA CGGCAAGAAT TTCACCGGAG TTTCTTTGTT TAAAGGAGAA 
GCTTTACCGG ATAAATTGTT GCCGTTTATT TACGCTGGGA ATGCTAGTAA 
TGCTACTAAT GGTAATCTCT GTATGACCGG AACTTTGATC CCGGAGAAAG 
TAAAGGGGAA GATTGTGATG TGTGATAGAG GAATTAATGC TAGAGTTCAG 
AAAGGTGATG TGGTTAAAGC AGCAGGTGGA GTTGGAATGA TTCTGGCTAA 
CACTGCGGCG AATGGTGAAG AGCTTGTTGC GGATGCTCAT TTGTTACCGG 
CGACCACCGT TGGTGAAAAA GCCGGTGATA TAATCCGGCA TTACGTGACT 
ACTGATCCTA ATCCCACCGC TTCGATTTCA ATCTTAGGAA CAGTCGTCGG 
TGTTAAACCA TCTCCGGTTG TCGCAGCGTT TAGCTCACGT GGACCGAATT 
CGATTACACC GAATATTCTT AAACCGGATC TGATCGCTCC TGGAGTAAAC 
ATCCTCGCCG CGTGGACCGG TGCTGCTGGA CCAACCGGAC TCGCTTCCGA 
TTCTCGCCGC GTGGAGTTCA ATATCATCTC TGGGACGTCG ATGTCTTGCC 
CTCACGTTAG TGGTTTAGCG GCGCTTCTCA AGTCGGTGCA TCCTGAATGG 
AGCCCGGCGG CGATTAGATC GGCGCTTATG ACCACCGCTT ACAAAACCTA 
CAAAGACGGT AAACCGTTAC TCGACATCGC GACAGGGAAG CCTTCGACGC 
CGTTCGATCA CGGTGCAGGA CACGTGTCAC CAACAACTGC CACTAATCCA 
GGACTCATCT ACGATCTAAC GACGGAGGAT TACTTAGGCT TCCTCTGCGC 
ATTGAATTAC ACATCGCCGC AAATTCGAAG TGTCTCGAGA CGTAATTACA 
CTTGCGATCC GAGTAAATCG TACTCGGTCG CTGATTTGAA CTACCCGTCG 
TTCGCCGTTA ACGTTGATGG AGTCGGTGCG TATAAGTACA CGCGCACGGT 
GACGAGCGTG GGAGGAGCTG GGACTTACTC GGTTAAAGTA ACTTCGGAGA 
CGACAGGAGT CAAGATTTCG GTTGAACCGG CGGTTTTGAA TTTCAAGGAA 
GCTAACGAGA AAAAATCGTA TACGGTGACG TTTACTGTAG ACTCGTCGAA 
GCCGTCTGGA TCTAACAGCT TTGGGAGTAT TGAATGGTCG GATGGGAAAC 
ACGTGGTGGG AAGTCCCGTG GCGATTAGCT GGACATAGTC GTAAATTATG 
AGAAATGAAA GGGGTTTTAT GGAAAAGAAA AGGTGTTTAC AGATTTATTG 
TAATGCCGTT TTTAGATACG AAGAAACCTC TGTTTAGCAA AAACGACATG 
CAGTCTGTTA AGACTTGCCT AGTTTGTTTT TGGGATGTCT TGCTGTTTTT 
AATTTTAAAA AGAAATATAT TCTCCGGCTA CAACAGTAGC TGGGTCTCTC 
TCAAAAAAAA AAAAAAAA

Gene models with high sequence identity

AT5G67360.1 [+] show detail - Subtilase family protein
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
superfamily	Peptidase S8/S53, subtilisin/kexin/sedolisin	9.1e-80
superfamily	Transferrin receptor ectodomain, apical domai	9.2e-08
superfamily	Proteinase inhibitor, propeptide	2.9e-07
HMMPanther	SUBTILISIN-LIKE PROTEASE (PLANT)	0
HMMPanther	Peptidase S8, subtilisin-related	0
Gene3D	Peptidase S8/S53, subtilisin/kexin/sedolisin	6.4e-72
FPrintScan	Peptidase S8/S53, subtilisin/kexin/sedolisin	2.7e-13
FPrintScan	Peptidase S8/S53, subtilisin/kexin/sedolisin	2.7e-13
FPrintScan	Peptidase S8/S53, subtilisin/kexin/sedolisin	2.7e-13
HMMPfam	Peptidase S8/S53, subtilisin/kexin/sedolisin	3.2e-30
HMMPfam	Proteinase inhibitor I9, subtilisin propeptide	1.4e-17
HMMPfam	Protease-associated PA	8.9e-13
PatternScan	Peptidase S8/S53, subtilisin, active site	0

See the detailed result >

AT5G67360.1

Model type: Protein coding
Short Description: Subtilase family protein
Curator Summary: Encodes a subtilisin-like serine protease essential for mucilage release from seed coats.
Computational Description: ARA12; FUNCTIONS IN: serine-type endopeptidase activity; INVOLVED IN: proteolysis, seed coat development, mucilage metabolic process involved seed coat development, mucilage extrusion from seed coat; LOCATED IN: extracellular region, apoplast, cell wall, plant-type cell wall; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 17 growth stages; CONTAINS InterPro DOMAIN/s: Protease-associated PA (InterPro:IPR003137), Proteinase inhibitor, propeptide (InterPro:IPR009020), Peptidase S8/S53, subtilisin/kexin/sedolisin (InterPro:IPR000209), Peptidase S8, subtilisin-related (InterPro:IPR015500), Peptidase S8/S53, subtilisin, active site (InterPro:IPR022398), Proteinase inhibitor I9, subtilisin propeptide (InterPro:IPR010259); BEST Arabidopsis thaliana protein match is: Subtilase family protein (TAIR:AT2G05920.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR