F28814

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28814

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28814

Determination of introduced cDNA(s)

  • RAFL06-79-G07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-79-G07 - Resource: pda03760

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-79-G07 - full reading 
    GCCATTACCG GGGAGAGAGT GAAATTAGGG CTGAGCGAGA GAGATTCTGA 
    AATAATTTCT TTTACGGCGG TGAAGATGAC TGAGCTAACG TCGGCGATGG 
    ACATTGACGT AGACGAAATT CAGCCTCGCA AGCCGATAAA CAAGGGCAAA 
    GACGTCGTCG GTTTTGGACC TCCGCCGCAG AGTAAGGCGA CGCCTTGGGT 
    TGAGAAGTAT CGACCTCAGT CGCTTGATGA CGTGGCTGCA CATCGTGACA 
    TTATTGATAC GATTGATAGG CTGACCAATG AGAACAAATT GCCCCATCTT 
    CTGTTGTATG GTCCTCCCGG TACTGGCAAA ACATCCACCA TTCTAGCTGT 
    TGCCCGGAAA CTATATGGAC CAAAGTACCG GAATATGATT CTTGAACTGA 
    ACGCATCAGA CGATAGAGGA ATCGATGTTG TAAGGCAGCA GATTCAAGAT 
    TTCGCTAGCA CGCAGAGTTT CTCTTTAGGA AAATCTTCGG TGAAGTTGGT 
    TTTGCTAGAT GAAGCAGATG CCATGACAAA AGATGCTCAG TTTGCCTTGC 
    GTAGAGTGAT TGAGAAATAC ACTAAGAGTA CTAGGTTTGC GCTCATTGGA 
    AACCATGTCA ATAAGATCAT CCCAGCTTTG CAGTCGAGAT GTACTCGATT 
    TAGATTTGCC CCTCTTGATG GAGTTCATAT GAGTCAACGT CTTAAACATG 
    TAATAGAAGC TGAAAGGCTT GTTGTAAGTG ACTGTGGTTT GGCAGCTCTT 
    GTACGGCTGA GCAATGGGGA CATGAGAAAA GCATTGAACA TTTTGCAGTC 
    AACGCATATG GCGTCAAAGG AAATTACAGA GGAAGAGTCA AAGCAAATTA 
    CAGAGGAAGA CGTTTACCTC TGCACCGGAA ACCCATTGCC CAAGGACATT 
    GAGCAGATAT CTCACTGGCT TCTGAATAAA CCATTTGATG AGTGCTACAA 
    AGACGTTTCA GAAATCAAGA CGAGAAAAGG ACTTGCCATT GTTGATATCG 
    TCAAAGAGAT TACCTTGTTT ATTTTTAAAA TCAAGATGCC TTCCGCTGTC 
    AGAGTTCAAC TGATAAACGA TTTGGCAGAC ATTGAGTACA GATTAAGTTT 
    TGGTTGCAAC GACAAACTGC AGCTTGGAGC TATCATTTCT ACTTTCACAC 
    ATGCCCGGTC TATCATTGTT GGTGCAGCAA AGTAAACCAT GTTTTTACCA 
    TTTTGGAACC CGCAGTAGCT CGCCACCTTA ACAAACCGCT AGAATGTTTT 
    ATCTCCGTGA TGTTTTCCTG ATAGTCTCTG TACATTTTGT GGCGTTTGTG 
    ACCAATAGGC TTGCTTTTTA TTTCTTATGA AGAGTTTCAA TGCCACCTTG 
    TTATCCTCTT CTCATCTCCT CGAATTTGTA ACTTTCAATT TCTTGATCAA 
    ATTATGTTTT AATGAAAAAA AAAAAAAAAA

    Gene models with high sequence identity

    1. AT1G77470.1 [+] show detail - replication factor C subunit 3
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily P-loop containing nucleoside triphosphate hyd 4.4e-53
    superfamily DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal 4.4e-26
    HMMSmart ATPase, AAA+ type, core 1.3e-11
    Gene3D no description 5.8e-51
    Gene3D no description 4.6e-13
    Gene3D no description 4.7e-20
    HMMPfam Replication factor C 4.3e-25
    HMMPfam ATPase, AAA-type, core 5.2e-16
    HMMPanther REPLICATION FACTOR C / DNA POLYMERASE III GAM 1.1e-48

    See the detailed result >

    AT1G77470.1

    Model type
    Protein coding
    Short Description
    replication factor C subunit 3
    Curator Summary
    Encodes a protein with high homology to the Replication Factor C, Subunit 3 (RFC3) of yeast and other eukaryotes. rfc3 mutants are hypersensitive to salicylic acid and exhibit enhanced induction of PR genes and resistance against virulent oomycete Hyaloperonospora arabidopsidis Noco2. The enhanced pathogen resistance in the mutant is NPR1-independent.
    Computational Description
    replication factor C subunit 3 (RFC3); FUNCTIONS IN: in 6 functions; INVOLVED IN: negative regulation of defense response; LOCATED IN: DNA replication factor C complex; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), Replication factor C (InterPro:IPR013748), DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal (InterPro:IPR008921); BEST Arabidopsis thaliana protein match is: ATPase family associated with various cellular activities (AAA) (TAIR:AT1G21690.1); Has 17584 Blast hits to 17541 proteins in 2810 species: Archae - 637; Bacteria - 9895; Metazoa - 902; Fungi - 991; Plants - 392; Viruses - 88; Other Eukaryotes - 4679 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR