F28814

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28814

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28814

Determination of introduced cDNA(s)

  • RAFL06-79-G07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-79-G07 - Resource: pda03760

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-79-G07 - full reading
    GCCATTACCG GGGAGAGAGT GAAATTAGGG CTGAGCGAGA GAGATTCTGA 
    AATAATTTCT TTTACGGCGG TGAAGATGAC TGAGCTAACG TCGGCGATGG
    ACATTGACGT AGACGAAATT CAGCCTCGCA AGCCGATAAA CAAGGGCAAA
    GACGTCGTCG GTTTTGGACC TCCGCCGCAG AGTAAGGCGA CGCCTTGGGT
    TGAGAAGTAT CGACCTCAGT CGCTTGATGA CGTGGCTGCA CATCGTGACA
    TTATTGATAC GATTGATAGG CTGACCAATG AGAACAAATT GCCCCATCTT
    CTGTTGTATG GTCCTCCCGG TACTGGCAAA ACATCCACCA TTCTAGCTGT
    TGCCCGGAAA CTATATGGAC CAAAGTACCG GAATATGATT CTTGAACTGA
    ACGCATCAGA CGATAGAGGA ATCGATGTTG TAAGGCAGCA GATTCAAGAT
    TTCGCTAGCA CGCAGAGTTT CTCTTTAGGA AAATCTTCGG TGAAGTTGGT
    TTTGCTAGAT GAAGCAGATG CCATGACAAA AGATGCTCAG TTTGCCTTGC
    GTAGAGTGAT TGAGAAATAC ACTAAGAGTA CTAGGTTTGC GCTCATTGGA
    AACCATGTCA ATAAGATCAT CCCAGCTTTG CAGTCGAGAT GTACTCGATT
    TAGATTTGCC CCTCTTGATG GAGTTCATAT GAGTCAACGT CTTAAACATG
    TAATAGAAGC TGAAAGGCTT GTTGTAAGTG ACTGTGGTTT GGCAGCTCTT
    GTACGGCTGA GCAATGGGGA CATGAGAAAA GCATTGAACA TTTTGCAGTC
    AACGCATATG GCGTCAAAGG AAATTACAGA GGAAGAGTCA AAGCAAATTA
    CAGAGGAAGA CGTTTACCTC TGCACCGGAA ACCCATTGCC CAAGGACATT
    GAGCAGATAT CTCACTGGCT TCTGAATAAA CCATTTGATG AGTGCTACAA
    AGACGTTTCA GAAATCAAGA CGAGAAAAGG ACTTGCCATT GTTGATATCG
    TCAAAGAGAT TACCTTGTTT ATTTTTAAAA TCAAGATGCC TTCCGCTGTC
    AGAGTTCAAC TGATAAACGA TTTGGCAGAC ATTGAGTACA GATTAAGTTT
    TGGTTGCAAC GACAAACTGC AGCTTGGAGC TATCATTTCT ACTTTCACAC
    ATGCCCGGTC TATCATTGTT GGTGCAGCAA AGTAAACCAT GTTTTTACCA
    TTTTGGAACC CGCAGTAGCT CGCCACCTTA ACAAACCGCT AGAATGTTTT
    ATCTCCGTGA TGTTTTCCTG ATAGTCTCTG TACATTTTGT GGCGTTTGTG
    ACCAATAGGC TTGCTTTTTA TTTCTTATGA AGAGTTTCAA TGCCACCTTG
    TTATCCTCTT CTCATCTCCT CGAATTTGTA ACTTTCAATT TCTTGATCAA
    ATTATGTTTT AATGAAAAAA AAAAAAAAAA

    Gene models with high sequence identity

    1. AT1G77470.1 [+] show detail - replication factor C subunit 3
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily P-loop containing nucleoside triphosphate hyd 4.4e-53
    superfamily DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal 4.4e-26
    HMMSmart ATPase, AAA+ type, core 1.3e-11
    Gene3D no description 5.8e-51
    Gene3D no description 4.6e-13
    Gene3D no description 4.7e-20
    HMMPfam Replication factor C 4.3e-25
    HMMPfam ATPase, AAA-type, core 5.2e-16
    HMMPanther REPLICATION FACTOR C / DNA POLYMERASE III GAM 1.1e-48

    See the detailed result >

    AT1G77470.1

    Model type
    Protein coding
    Short Description
    replication factor C subunit 3
    Curator Summary
    Encodes a protein with high homology to the Replication Factor C, Subunit 3 (RFC3) of yeast and other eukaryotes. rfc3 mutants are hypersensitive to salicylic acid and exhibit enhanced induction of PR genes and resistance against virulent oomycete Hyaloperonospora arabidopsidis Noco2. The enhanced pathogen resistance in the mutant is NPR1-independent.
    Computational Description
    replication factor C subunit 3 (RFC3); FUNCTIONS IN: in 6 functions; INVOLVED IN: negative regulation of defense response; LOCATED IN: DNA replication factor C complex; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), Replication factor C (InterPro:IPR013748), DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal (InterPro:IPR008921); BEST Arabidopsis thaliana protein match is: ATPase family associated with various cellular activities (AAA) (TAIR:AT1G21690.1); Has 17584 Blast hits to 17541 proteins in 2810 species: Archae - 637; Bacteria - 9895; Metazoa - 902; Fungi - 991; Plants - 392; Viruses - 88; Other Eukaryotes - 4679 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR