F29238

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F29238

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F29238

Determination of introduced cDNA(s)

  • RAFL09-79-N19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-79-N19 - Resource: pda09917

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-79-N19 - full reading 
    ACCAATCTCT CACAAGTCAC AACATATCCA TTTTCCAGGA AGCTTTGGAA 
    GCACATGATG GAGCAAGTAA AGGGAAATAC ACTATTGGAC TTGGCCAAGA 
    TTGTTTAGCT TTTTGCACTG AGCTTGAAGA TGTTATCTCT ATGAGTTTCA 
    ATGCGGTGAC ATCACTTTTT GAGAAGTATA AGATTGACCC TAACCAAATC 
    GGGCGTCTTG AAGTAGGAAG TGAGACTGTT ATTGACAAAA GCAAGTCCAT 
    CAAGACCTTC TTGATGCAGC TCTTTGAGAA ATGTGGAAAC ACTGATGTCG 
    AAGGTGTTGA CTCGACCAAT GCTTGCTATG GTGGAACTGC AGCTTTGTTA 
    AACTGTGTCA ATTGGGTTGA GAGTAACTCT TGGGATGGAC GTTATGGCCT 
    CGTCATTTGT ACTGACAGCG CGGTTTATGC AGAAGGACCC GCAAGGCCCA 
    CTGGAGGAGC TGCAGCGATT GCTATGTTGA TAGGACCTGA TGCTCCTATC 
    GTTTTCGAAA GCAAATTGAG AGCAAGCCAC ATGGCTCATG TCTATGACTT 
    TTACAAGCCC AATCTTGCTA GCGAGTACCC GGTTGTTGAT GGTAAGCTTT 
    CACAGACTTG CTACCTCATG GCTCTTGACT CCTGCTATAA ACATTTATGC 
    AACAAGTTCG AGAAGATCGA GGGCAAAGAG TTCTCCATAA ATGATGCTGA 
    TTACATTGTT TTCCATTCTC CATACAATAA ACTTGTACAG AAAAGCTTTG 
    CTCGTCTCTT GTACAACGAC TTCTTGAGAA ACGCAAGCTC CATTGACGAG 
    GCTGCCAAAG AAAAGTTCAC CCCTTATTCA TCTTTGACCC TTGACGAGAG 
    TTACCAAAGC CGTGATCTTG AAAAGGTGTC ACAACAAATT GCGAAACCGT 
    TTTATGATGC TAAAGTGCAA CCAACGACTT TAATACCAAA GGAAGTCGGT 
    AACATGTACA CTGCTTCTCT CTACGCTGCA TTTGCTTCCC TCATCCACAA 
    GAAACACAAT GATTTGGCGG GAAAGCGGGT GGTTATGTTC TCTTATGGAA 
    GTGGCTCAAC CGCAACAATG TTCTCATTAC GCCTCAACGA CAATAAGCCT 
    CCTTTCAGCA TTTCAAACAT TGCATCTGTA ATGGATGTTG GCGGTAAATT 
    GAAAGCTAGA CATGAGTATG CACCTGAGAA GTTTGTGGAG ACAATGAAGC 
    TAATGGAACA TAGGTATGGA GCAAAGGACT TTGTGACAAC CAAGGAGGGT 
    ATTATAGATC TTTTGGCACC GGGAACTTAT TATCTGAAAG AGGTTGATTC 
    CTTGTACCGG AGATTCTATG GCAAGAAAGG TGAAGATGGA TCTGTAGCCA 
    ATGGACACTG ATTCTTGAAA AGGAACAAAT CATAAAAAGT CACCTGAAAA 
    TGTTATGTTT TACTTGCACC ATTTTAATCG TTTTATAAGT TTGTTGTTAC 
    TTTGATTTAG GGACAAAGAA GGAATGTATT CTCTGTTTTT ATTTACTGTT 
    TGATTTACTC TCTCATGCAA ATGATAATTT GGAAATTATT GGAATCTCTA 
    TTTTGTATGT TTGTGTTCTT

    Gene models with high sequence identity

    1. AT4G11820.1 [+] show detail - hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A syn...
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Hydroxymethylglutaryl-coenzyme A synthase, active site 0
    HMMTigr Hydroxymethylglutaryl-CoA synthase, eukaryotic 0
    superfamily Thiolase-like 6.9e-62
    superfamily Thiolase-like 2.4e-26
    HMMPanther HYDROXYMETHYLGLUTARYL-COA SYNTHASE 5.8e-221
    HMMPfam Hydroxymethylglutaryl-coenzyme A synthase C-terminal 3.4e-121
    HMMPfam Hydroxymethylglutaryl-coenzyme A synthase, N-terminal 7.5e-71

    See the detailed result >

    AT4G11820.1

    Model type
    Protein coding
    Short Description
    hydroxymethylglutaryl-CoA synthase / HMG-CoA synthase / 3-hydroxy-3-methylglutaryl coenzyme A synthase
    Curator Summary
    Encodes a protein with hydroxymethylglutaryl-CoA synthase activity which was characterized by phenotypical complementation of the S. cerevisiae mutant.
    Computational Description
    HYDROXYMETHYLGLUTARYL-COA SYNTHASE (HMGS); CONTAINS InterPro DOMAIN/s: Hydroxymethylglutaryl-coenzyme A synthase C-terminal (InterPro:IPR013746), Thiolase-like (InterPro:IPR016039), Hydroxymethylglutaryl-coenzyme A synthase, N-terminal (InterPro:IPR013528), Hydroxymethylglutaryl-CoA synthase, eukaryotic (InterPro:IPR010122), Hydroxymethylglutaryl-coenzyme A synthase, active site (InterPro:IPR000590); Has 2141 Blast hits to 2138 proteins in 847 species: Archae - 222; Bacteria - 1037; Metazoa - 301; Fungi - 182; Plants - 116; Viruses - 0; Other Eukaryotes - 283 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR