F29910

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	cauline leaf (PO:0000013)	increased width (PATO:0000600) (Cauline leaf::Shape::wide)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) abnormal (PATO:0000460) (Rosette leaf::Others) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many) abnormal (PATO:0000460) (Rosette leaf::Others)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F29910

Determination of introduced cDNA(s)

RAFL14-56-E23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL14-56-E23 - Resource: pda18782

full reading [+] show sequence

GTGAATTCAC AGTTGAGCGA GATGAAAAGT ACGGTGGTAA CAGGACCTTC 
AAAAGCTTTG AAGACATCAC CACTGACTAT GAAAGCGGCC AGTTGCACCC 
AAAGGATTTG AAGGACGCTC TGTCAAAAGC GTTGAATAAG ATACTGCAAC 
CTGTTCGTGA TCATTTCAAA CCAACAGCCG CGCCAAGAAT CTGCTCAAAC 
AGGTCAAGGG TTACAAAGTC ACCAGAGTCA TACCAACAGC TTCATCTACA 
AAGGAGGAGG ATCTTTCCAT CAACACATCA GCATCTTCTT CGGCCGCCGG 
ATTACAGATG AGCGAGGAGG CCGAGATGAA ATACAAGATT GTGAGGAGTA 
TTGGTGAGGA ATGCATACAA GAGGATGAGC TGAAGAATCT TCTAGCCAAG 
AAGCCTGCTC CTATCTGCTA CGATGGTTTT GAACCATCAG GAAGGATGCA 
CATCGCCCAG GGAGTTATGA AGGTCACCAA TGTGAACAAA TTGACTTCAG 
CTGGTTGCCA AGTGAAAATT TGGATAGCAG ATTGGTTTGC TCAATTGAAC 
AACAAGTTGG GAGGTGACTT GGAGAGAATT AGAGTGGTAG GAGAATATTT 
CAAGGAAATT TGGCAAGCAG GTGGGATGAA CAATGACAAA GTAGAGTTTC 
TTTGGGCATC AGACGAAATA AATGGCAAGG GGAGTAAGTA TTGGCCTCTT 
GTGATGGACA TTGCTCGTAG AAACAACCTA CGTCGAATCT TGAGGTGTGG 
GCAGATTATG GGACGTAGTG AGACTGAGGT GCTGAGTGCA GCTCAAATTC 
TTTACCCCTG TATGCAGTGT GCGGATATTT TCTTACTTGA GGCTGATATT 
TGCCAGCTCG GGATGGATCA GCGAAAAGTA AACATGCTAG CAAGAGAATA 
CTGTGCTGAC ATTAAGAGGA AAAATAAACC CATCATCTTG TCCCACCATA 
TGCTTCCTGG TCTCCAACAA GGACAAGAAA AGATGTCCAA AAGTGATCCA 
TCATCTGCAA TATTTATGGA AGATGAGGAG GCTGATGTTA ATGAGAAGAT 
CAGCAAAGCC TATTGCCCTC CGAAAACTGT GGAAGGCAAT CCATGTCTCG 
AGTATGTCAA ATACATTGTT CTACCACGAT TCAATGAGTT CAAGGTTGAA 
AGTGAAAAGA ATGGCGGCAA TAAGACCTTC AACAGCTTTG AAGATATTGT 
CGCCGACTAC GAGACCGGCG AGCTACATCC CGAGGATCTG AAGAAAGCTC 
TGATGAAAGC ATTGAATATA ACTCTGCAAC CTGTTCGCGA TCACTTTAAA 
ACCAACGAGC GTGCCAAAAA TCTACTGGAA CAGGTCAAGG CTTTTAGAGT 
CACCAGGTGA AGTTGCAGAA GCAAAGAGAG TATTTGTGGA CATCAGTTAA 
AGAGCTTTCC TAAGGATTTT GGTGGTTACT AAGAAAGCTA ACAAATCTCT 
TAAAGTTCAT TTTTGTTTTT TGATAGATAT GAGCCTCGAA GATTAGAATC 
CAGTTGGACA AATTTTGTGA TTTATTGTCA ACTAAAAATA AACCTCCACA 
GCTACAAGAG CATTAAACTA AAATTTTCCA GTAGAAAAA

Gene models with high sequence identity

AT1G28350.1 [+] show detail - Nucleotidylyl transferase superfamily protein
E-value: 0; Score: 99.87

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Aminoacyl-tRNA synthetase, class Ib	2e-65
HMMPfam	Aminoacyl-tRNA synthetase, class Ib	0.000095
HMMPIR	Tyrosine tRNA ligase, archaeal/eukaryotic	8.7e-203
superfamily	Nucleotidylyl transferase	2.7e-85
superfamily	Nucleotidylyl transferase	0.00000000055
HMMPanther	Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic	3.5e-162
HMMPanther	ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHION	3.5e-162
Gene3D	Rossmann-like alpha/beta/alpha sandwich fold	5.6e-68
Gene3D	no description	6.8e-39
Gene3D	no description	0.0000000000003

See the detailed result >

AT1G28350.1

Model type: Protein coding
Short Description: Nucleotidylyl transferase superfamily protein
Computational Description: Nucleotidylyl transferase superfamily protein; FUNCTIONS IN: tyrosine-tRNA ligase activity, nucleotide binding, aminoacyl-tRNA ligase activity, ATP binding; INVOLVED IN: translation, tyrosyl-tRNA aminoacylation, tRNA aminoacylation for protein translation; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Tyrosyl-tRNA synthetase, class Ib, archaeal/eukaryotic cytosolic (InterPro:IPR015624), Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial (InterPro:IPR002307), Aminoacyl-tRNA synthetase, class Ib (InterPro:IPR002305); BEST Arabidopsis thaliana protein match is: Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial (TAIR:AT2G33840.1); Has 6761 Blast hits to 4745 proteins in 1539 species: Archae - 718; Bacteria - 3084; Metazoa - 577; Fungi - 521; Plants - 241; Viruses - 10; Other Eukaryotes - 1610 (source: NCBI BLink).
Link: InterPro Scan - TAIR