F30415

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F30415

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F30415

Determination of introduced cDNA(s)

  • RAFL05-04-H19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-04-H19 - Resource: pda00686

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-04-H19 - full reading 
    CACGAGATTT TTTATTTTAA TATCACGCTC GAATATACAA TTCGGAATGT 
    GCAGATGTTT TTTTTGAAGG CAATTTAGAA AAATAGACAA AAATCGAACA 
    AACATATATA GAAATAGAGA TCTAAATAAA AACATTTATT TTATTTCCTC 
    TTGGAAACAA GGAAAAAATG AATTGGGGTG GGTGACGTGG CAGTCATACC 
    CTTGCCACAC CCTTCCTATA CGCATATAAA CAGCAGCTGC GAGGAGAGAG 
    AGATTTCAGA GTATTTTCTA GGGTTTCGTT TTTTTAAAGA TTTAGAGAAA 
    AAAGTGAGTT ATTAAGAGAT TCCAATCAAA ATGAGCGGAG ACAACGGCGG 
    TGGTGAGAGG CGCAAAGGCT CCGTCAAGTG GTTTGATACC CAGAAGGGTT 
    TCGGCTTCAT CACTCCTGAC GACGGTGGCG ACGATCTCTT CGTTCACCAG 
    TCCTCCATCA GATCTGAGGG TTTCCGTAGC CTCGCTGCCG AAGAAGCCGT 
    AGAGTTCGAG GTTGAGATCG ACAACAACAA CCGTCCCAAG GCCATCGATG 
    TTTCTGGACC CGACGGCGCT CCCGTCCAAG GAAACAGCGG TGGTGGTTCA 
    TCTGGCGGAC GCGGCGGTTT CGGTGGAGGA AGAGGAGGTG GACGCGGATC 
    TGGAGGTGGA TACGGCGGTG GCGGTGGTGG ATACGGAGGA AGAGGAGGTG 
    GTGGTCGAGG AGGCAGCGAC TGCTACAAGT GTGGTGAGCC CGGTCACATG 
    GCGAGAGACT GTTCTGAAGG CGGTGGAGGT TACGGAGGAG GCGGCGGTGG 
    CTACGGAGGT GGAGGCGGAT ACGGCGGAGG AGGTGGTGGT TACGGAGGTG 
    GTGGCCGTGG AGGTGGTGGC GGCGGGGGAA GCTGCTACAG CTGTGGCGAG 
    TCGGGACATT TCGCCAGGGA TTGCACCAGC GGTGGACGTT AAAACCAACG 
    CCGGTTACGC GGTGGAGAAG AGTGAGTTGG TTATCTCACA AGTGATCGGT 
    TCTTTCTCCC GCCGCCTTCT ATCTCTCTAT TATCCACTTT TTGCTTATTA 
    TGATGGATCT CTATCTTTGT TAGTTGGTTT TTTCTTGAT

    Gene models with high sequence identity

    1. AT4G38680.1 [+] show detail - glycine rich protein 2
      E-value: 0; Score: 99.91

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Cold-shock protein, DNA-binding 7.5e-11
    FPrintScan Cold-shock protein, DNA-binding 7.5e-11
    FPrintScan Cold-shock protein, DNA-binding 7.5e-11
    PatternScan Cold-shock conserved site 0
    HMMSmart Cold shock protein 8.9e-23
    HMMSmart Zinc finger, CCHC-type 1.3e-05
    HMMSmart Zinc finger, CCHC-type 3.4e-05
    HMMPanther GLYCINE-RICH PROTEIN 2 2e-82
    HMMPanther ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN 2e-82
    Gene3D Nucleic acid-binding, OB-fold 1.6e-13
    HMMPfam Cold-shock protein, DNA-binding 2.4e-26
    HMMPfam Zinc finger, CCHC-type 2.5e-08
    HMMPfam Zinc finger, CCHC-type 1.2e-07
    ProfileScan Zinc finger, CCHC-type 11.499
    ProfileScan Zinc finger, CCHC-type 10.938
    superfamily Nucleic acid-binding, OB-fold-like 6.7e-21
    superfamily Retrovirus zinc finger-like domains 2.1e-10

    See the detailed result >

    AT4G38680.1

    Model type
    Protein coding
    Short Description
    glycine rich protein 2
    Curator Summary
    Encodes a glycine-rich protein that binds nucleic acids and promotes DNA melting. Its transcript and protein levels are up-regulated in response to cold treatment with protein levels peaking earlier in shoots (~10-14 days) than in roots (~21 days). It is normally expressed in meristematic regions and developing tissues where cell division occurs. RNAi and antisense lines with lower levels of CSP2/GRP2 transcripts flower earlier than wild type plants and have some defects in anther and seed development.
    Computational Description
    glycine rich protein 2 (GRP2); FUNCTIONS IN: double-stranded DNA binding, mRNA binding, single-stranded DNA binding, nucleic acid binding; INVOLVED IN: stamen development, vegetative to reproductive phase transition of meristem, response to cold, seed development, DNA duplex unwinding; LOCATED IN: nucleolus, nucleus, plasma membrane, cytoplasm; EXPRESSED IN: 37 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Nucleic acid-binding, OB-fold (InterPro:IPR012340), Cold shock protein (InterPro:IPR011129), Cold-shock conserved site (InterPro:IPR019844), Zinc finger, CCHC-type (InterPro:IPR001878), Cold-shock protein, DNA-binding (InterPro:IPR002059); BEST Arabidopsis thaliana protein match is: glycine-rich protein 2B (TAIR:AT2G21060.1); Has 150401 Blast hits to 55289 proteins in 3379 species: Archae - 285; Bacteria - 51625; Metazoa - 48133; Fungi - 9113; Plants - 13783; Viruses - 1771; Other Eukaryotes - 25691 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR