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GT12732

CSHL Arabidopsis genetrap mutant lines

Original: GT12732

Phenotypes

Morphological phenotypes

Original description

  • embryo lethal
Anatomical entity
/structure development stage
Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: GT12732

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 21553870 [Map]; Strand: w
      • AT1G58200.1 - [+] show detail - MSCS-like 3
        Distance: -785; Region: promoter
      • AT1G58200.2 - [+] show detail - MSCS-like 3
        Distance: -743; Region: promoter
      • AT1G58210.1 - [+] show detail - kinase interacting family protein
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT12732 - 5-prime edge
    TCAAAACCCT AAAATCCGTC ACATTGTGCG ATTGGTGGCT AACAAAGAAA 
    GGCAAAGATT TGTGTATTAA AGGATTCGAA TCAAATGGTG CATCTGCGAG
    TAAGACTGTT TTCATCAGGA ACAATCTCAA AGCGACATGA AAGTACAACT
    CTTGAAGCAA TTGATGGGAT TACAATTTCT ATCAATGGTT TCATCAATCG
    ATCTCGTTGT CTAGAGAATG GTATTTCGAT TGAGGTTTGT AATCGTTTTC
    GTTTAGGGTT TCCTTATGAT TGGGAAGATT ACAATGAAGA AGAAGAAGAG
    AAGAAGAAGA AGAATGTTGA TATTTCGTTT GATGATATTC CTGTGAATAG
    GTATCACCAT CTTCACTCTC CTTAACTGCA ATAACATCTT TCTTCGTTTT
    CTCCGTTGAG ACTGACTTCA AATCATCTCG AGAATCTTCT GATCCTGAGA
    CAACAGGCAA CTCCTGGAAA ATTCCAGCTC CTTCAACATC TTCATCCATC
    TTCACATCTT GTATCTTGCC AGATAAATCA TCAACAGTCC TATTAGCTAC
    CTTGAATTGG TTCTGTAGAT TCTTGTTTTG ATCTTCCACT TTCCGAAATA
    GTTTTCTAAC ATTTCTCCAG CTCGTCTTCA AGAACCGTTA TCCTCTGTTT
    CAT

AT1G58200.1

Model type
Protein coding
Short Description
MSCS-like 3
Curator Summary
A member of MscS-like gene family, structurally very similar to MSL2, comprising of an N-terminal chloroplast transit peptide, five trans-membrane helices and a C-terminal cytoplasmic domain. Mutant plants showed abnormalities in the size and shape of plastids. MSL3-GFP was localized to discrete foci on the plastid envelope and co-localize with the plastid division protein AtMinE. MSL3 was capable of increasing the osmotic-shock survival of a mutant bacterial strain lacking MS-ion-channel activity.
Computational Description
MSCS-like 3 (MSL3); FUNCTIONS IN: ion channel activity; INVOLVED IN: plastid organization, response to osmotic stress; LOCATED IN: membrane, plastid envelope; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Mechanosensitive ion channel MscS (InterPro:IPR006685), Like-Sm ribonucleoprotein (LSM)-related domain (InterPro:IPR010920); BEST Arabidopsis thaliana protein match is: MSCS-like 2 (TAIR:AT5G10490.1); Has 6506 Blast hits to 5934 proteins in 1329 species: Archae - 154; Bacteria - 2583; Metazoa - 875; Fungi - 404; Plants - 332; Viruses - 16; Other Eukaryotes - 2142 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G58200.2

Model type
Protein coding
Short Description
MSCS-like 3
Curator Summary
A member of MscS-like gene family, structurally very similar to MSL2, comprising of an N-terminal chloroplast transit peptide, five trans-membrane helices and a C-terminal cytoplasmic domain. Mutant plants showed abnormalities in the size and shape of plastids. MSL3-GFP was localized to discrete foci on the plastid envelope and co-localize with the plastid division protein AtMinE. MSL3 was capable of increasing the osmotic-shock survival of a mutant bacterial strain lacking MS-ion-channel activity.
Computational Description
MSCS-like 3 (MSL3); FUNCTIONS IN: ion channel activity; INVOLVED IN: plastid organization, response to osmotic stress; LOCATED IN: membrane, plastid envelope; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Mechanosensitive ion channel MscS (InterPro:IPR006685), Like-Sm ribonucleoprotein (LSM)-related domain (InterPro:IPR010920); BEST Arabidopsis thaliana protein match is: MSCS-like 2 (TAIR:AT5G10490.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G58210.1

Model type
Protein coding
Short Description
kinase interacting family protein
Computational Description
EMBRYO DEFECTIVE 1674 (EMB1674); CONTAINS InterPro DOMAIN/s: SANT associated (InterPro:IPR015216), KIP1-like (InterPro:IPR011684); BEST Arabidopsis thaliana protein match is: Kinase interacting (KIP1-like) family protein (TAIR:AT1G09720.1); Has 30818 Blast hits to 19641 proteins in 1442 species: Archae - 565; Bacteria - 3414; Metazoa - 15518; Fungi - 2982; Plants - 1779; Viruses - 78; Other Eukaryotes - 6482 (source: NCBI BLink).
Link
InterPro Scan - TAIR