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GT13762

CSHL Arabidopsis genetrap mutant lines

Original: GT13762

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT13762

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 21609459 [Map]; Strand: w
      • AT5G53280.1 - [+] show detail - plastid division1
        Distance: 41; Region: intergenic
      • AT5G53290.1 - [+] show detail - cytokinin response factor 3
        Distance: 8354; Region: intergenic
    Flanking Genome Sequence: GT13762 - 3-prime edge
    TATTTTACCG CACCGTTACC GACCGTTTTC ATCCCTAGAT TTGTCATCAA 
    AAGTAAAAGG GCCTAAGATT GAGCAAACTA AGGCTGGATA GTTCTGGGAT
    GCGAATGGGC TGATAGTGGC CCAATACATT CGTCAATCTG AATTTGCCAG
    TTGCTACTAA AGAACAGCCG AGAGATCTTG TGATTTACAA GAAAGCCTAA
    CCACACTTGA AATCAACAAA ACCAAACAAA ACAAACAAAA CATTAACCAC
    AGTGATTGAA AATCAAAATT AGAAGAAAGG AACAATATTT GCATCAGACG
    AGCTCAGAAA ATTCCAACAC TTTGCCTCCA AATTCTTATC CCTTTGCTAA
    TAATATCGAA ACCTTTTGAG AGCAACAATA TCTCTTCTCT TC
  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 21609564 [Map]; Strand: w
      • AT5G53280.1 - [+] show detail - plastid division1
        Distance: 146; Region: intergenic
      • AT5G53290.1 - [+] show detail - cytokinin response factor 3
        Distance: 8249; Region: intergenic
    Flanking Genome Sequence: GT13762 - 5-prime edge
    TTGGTCCGGA AACCATATAC AATATACAGT ACGAATACTG TAGAGGTTTA 
    TCGTCTAGTT TTTGTTATTC AAATTGAAGA AAGATAACGT TTTCTAGACA
    TTATGAGTTA TATGACTTGT ATTCGTAGTT TAATATCAAC TTTTTTTACT
    CTTTCTTTGT TTCTACAAAG CCTGAATATC TATAATGTAT AAGGGTGTCA
    TTGTCAAAGC GGGAGAGTTT GTCCGCCGTA TGTGAATTGT GATGTGAACA
    AAGCAAAGAT AAGTGGGAGG ATAAGGTATC AAGATGATAA AGGGGAAACT
    ACAATAAGTC CAAATTGGAA GAATGAAGGA AACATGAATG TGAATATAAC
    AAAATACTTT TCCTTAAGAT CTGTTTTGTC TCTCTTTTTC TTTTGCTTTT
    GTACACATAG CTTTTTCTCT ACACTCTTAA CGTTATAAAT GGCTTCTTCT
    TTTT

AT5G53280.1

Model type
Protein coding
Short Description
plastid division1
Curator Summary
An integral outer envelope membrane protein (as its homolog PDV2), component of the plastid division machinery. Similar to ARC5, PDV1 localized to a discontinuous ring at the division site in wild-type plants. PDV1 and PDV2 are required for localization of ARC5 at the chloroplast division site. Topological analysis showed that the large N-terminal region of PDV1 upstream of the transmembrane helix bearing a putative coiled-coil domain is exposed to the cytosol. Mutation of the conserved PDV1 C-terminal Gly residue did not block PDV1 insertion into the outer envelope membrane but did abolish its localization to the division site.
Computational Description
PLASTID DIVISION1 (PDV1); BEST Arabidopsis thaliana protein match is: plastid division2 (TAIR:AT2G16070.2); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G53290.1

Model type
Protein coding
Short Description
cytokinin response factor 3
Curator Summary
encodes a member of the ERF (ethylene response factor) subfamily B-5 of ERF/AP2 transcription factor family. The protein contains one AP2 domain. There are 7 members in this subfamily.
Computational Description
cytokinin response factor 3 (CRF3); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: cytokinin response factor 4 (TAIR:AT4G27950.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR