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Mutant Lines
GT16075

GT16075

CSHL Arabidopsis genetrap mutant lines

Original: GT16075

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT16075

Determination of insertion point(s)

3 prime edge [+] show sequence

Not mapped
Flanking Genome Sequence: GT16075 - 3-prime edge
```
TCCGACCGTT TTCATCCCTA TTAACATCGT AGTAGAACTC A
```
3 prime edge [+] show sequence
1. Chromosome: 2; Position: 11175130 [Map]; Strand: c
  - AT2G26250.1 - [+] show detail - 3-ketoacyl-CoA synthase 10
    Distance: -1988; Region: intergenic
  - AT2G26260.1 - [+] show detail - 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
    Distance: -2970; Region: intergenic
Flanking Genome Sequence: GT16075 - 3-prime edge
```
TTTTCATCCC TATTAACATC GTAGTAGAAC TCAAAGCGAG ATGACGCTTC 
CAAAGGGGTA AATAATTCTT G
```

5 prime edge [+] show sequence

Chromosome: 2; Position: 11175046 [Map]; Strand: c
- AT2G26250.1 - [+] show detail - 3-ketoacyl-CoA synthase 10
  Distance: -1904; Region: intergenic
- AT2G26260.1 - [+] show detail - 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
  Distance: -3054; Region: intergenic

TCCTACTTTC TCCCTGGATG TTAATTATGG TTAGACTCCC ACGGTGGGAG 
TTCCCATTTA CTCTTAAATG CAACTGAACG GGCTTTACTA TTTGATGCAA 
TGATACAAAG AACCTTCAGA TTTAAACAAT GAGACGATCG CAACACAAGA 
GAATAATGTT ACTGATCCTT CTACAACAAA CACTGCACCA AGGCCTAGTT 
TGCTTGATTG AATGAAGTAT ACAAAGCATA GACACTTATC ACATTTAAAG 
TATTTGTAAT ATGGCAATTT CGTATGTGTT AACACATTTC TCAAATTTTA 
AAAATCGTTT ATATGGATCT TATAAAATAT GGTAGTAATA ATTTAGGTGG 
GGGATTGGGT CATTATTTAT TTCTCTTCTA TTTTGCTACT GCATTAACTA 
ATATTAGGCT AAAAGTTCCT TAAATAATAA AGCTTTTTTA TGCGTAATGT 
AGCCGCCCTC ATTTATATAG GTCTCACCAC CAATCGATTA ATTGTAAGTA 
ACAGAAATTG TTGATTATAG TTAAATGAGT CGTTGATAAT TTGTTTAAAT 
TTGTAAACTC TTCAGGCAAA ATTTATATTA TGTGAACAAA ATTGCCATAT 
ATTT

AT2G26250.1

Model type: Protein coding
Short Description: 3-ketoacyl-CoA synthase 10
Curator Summary: epidermis-specific, encodes KCS10, a putative 3-ketoacyl-CoA synthase. probably involved in the synthesis of long-chain lipids found in the cuticle.
Computational Description: 3-ketoacyl-CoA synthase 10 (KCS10); FUNCTIONS IN: transferase activity, transferring acyl groups other than amino-acyl groups, catalytic activity, acyltransferase activity; INVOLVED IN: in 8 processes; LOCATED IN: endoplasmic reticulum, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase-like (InterPro:IPR016039), Very-long-chain 3-ketoacyl-CoA synthase (InterPro:IPR012392), 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (InterPro:IPR013747), FAE1/Type III polyketide synthase-like protein (InterPro:IPR013601), Thiolase-like, subgroup (InterPro:IPR016038); BEST Arabidopsis thaliana protein match is: 3-ketoacyl-CoA synthase 4 (TAIR:AT1G19440.1); Has 1723 Blast hits to 1687 proteins in 351 species: Archae - 0; Bacteria - 632; Metazoa - 0; Fungi - 0; Plants - 985; Viruses - 0; Other Eukaryotes - 106 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT2G26260.1

Model type: Protein coding
Short Description: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Curator Summary: Encodes an enzyme with 3β-hydroxysteroid dehydrogenase/C4-decarboxylase activity in vitro. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the Arabidopsis gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
Computational Description: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 (3BETAHSD/D2); FUNCTIONS IN: 3-beta-hydroxy-delta5-steroid dehydrogenase activity, sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; INVOLVED IN: steroid biosynthetic process, metabolic process; LOCATED IN: membrane; EXPRESSED IN: 12 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: Reticulon (InterPro:IPR003388), NAD(P)-binding domain (InterPro:IPR016040), 3-beta hydroxysteroid dehydrogenase/isomerase (InterPro:IPR002225); BEST Arabidopsis thaliana protein match is: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (TAIR:AT1G47290.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR