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GT16265

CSHL Arabidopsis genetrap mutant lines

Original: GT16265

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT16265

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 6589148 [Map]; Strand: c
      • AT5G19530.1 - [+] show detail - S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
        Distance: 0; Region: exon9 (3UTR)
      • AT5G19530.2 - [+] show detail - S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
        Distance: 0; Region: exon10 (3UTR)
    Flanking Genome Sequence: GT16265 - 5-prime edge 
    CCTACTTTCT CCCTGGTCAT GGTGTGGCGT ACCGGCATAT TTAAAGACGA 
    ACCGGTTTCA GTTTCAGTGT TATTACCAAA CCCATGTCAC AAAAACAAAA 
    GGCCGGTTTC TTTTCTCCGC ACAGAACCGG GTGTTGTCTT GAATCTTGAT 
    TACTTTGGTT CGGTTTTATT TTCTACATTG CTTTTTGTTT TCTTGTTCAT 
    CCNCACTCGA CAATCCGGTT TAACAAGACT ATATTGCTTA CTAAGAAAGA 
    CCGATCTTCA ATTACTTATT TCTCCACACC AAATCTAGAA ATCGAAATAG 
    GCCAATTATG GTTCGATTAA ACCGAAGCAA TTAAAGTAAA CCCGAGTTTC 
    TGAGTGCAAT TAAACCGAAA CAAATCAAAT TTAAACCGAA GCAAATGAAG 
    GAAACTAGTA CTAGTGGAAT AAAATGCAAA AGAAGAGAAG GAAAACACGA 
    TCATTTGTGA CCAGTGAGAT TGACATCTTG AGGAAGGAGA GTGTAATCGA 
    TCTCAAGATC CTCGAGAATT CGCTTGATTG CGATGATCAG CGCGGTTCTT 
    CTTAAACTCT TCTCTACATA TACTTGGAAA TTTATCGTGT GTTGGACCAG 
    TATATTCAAT ACCAGC

AT5G19530.1

Model type
Protein coding
Short Description
S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Curator Summary
Encodes a spermine synthase. Required for internode elongation and vascular development, specifically in the mechanism that defines the boundaries between veins and nonvein regions. This mechanism may be mediated by polar auxin transport. Though ACL5 has been shown to function as a spermine synthase in E. coli, an ACL5 knockout has no effect on the endogenous levels of free and conjugated polyamines in Arabidopsis, suggesting that ACL5 may have a very specific or altogether different in vivo function.
Computational Description
ACAULIS 5 (ACL5); CONTAINS InterPro DOMAIN/s: Spermine synthase (InterPro:IPR001045); BEST Arabidopsis thaliana protein match is: spermidine synthase 1 (TAIR:AT1G23820.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G19530.2

Model type
Protein coding
Short Description
S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Curator Summary
Encodes a spermine synthase. Required for internode elongation and vascular development, specifically in the mechanism that defines the boundaries between veins and nonvein regions. This mechanism may be mediated by polar auxin transport. Though ACL5 has been shown to function as a spermine synthase in E. coli, an ACL5 knockout has no effect on the endogenous levels of free and conjugated polyamines in Arabidopsis, suggesting that ACL5 may have a very specific or altogether different in vivo function.
Computational Description
ACAULIS 5 (ACL5); CONTAINS InterPro DOMAIN/s: Spermine synthase (InterPro:IPR001045); BEST Arabidopsis thaliana protein match is: spermidine synthase 1 (TAIR:AT1G23820.1); Has 4823 Blast hits to 4823 proteins in 1442 species: Archae - 170; Bacteria - 2608; Metazoa - 258; Fungi - 194; Plants - 443; Viruses - 0; Other Eukaryotes - 1150 (source: NCBI BLink).
Link
InterPro Scan - TAIR