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GT16272

CSHL Arabidopsis genetrap mutant lines

Original: GT16272

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT16272

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 181662 [Map]; Strand: w
      • AT2G01420.1 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon2 (CDS)
      • AT2G01420.2 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: GT16272 - 3-prime edge
    AACGGTAGAG GATTTTACCG ACCGTTACCG ACCGTTTTCA TCCATAATAT 
    TGATAATGGT GTGGAGAAAG CTGATCAGAA ACCCAAACAC GTACTCCAGT
    CTAATCGGTC TCATATGGGC TCTTGTTGCT TACCGGTCAG TGAGTCCAAT
    CATTTTCTTA CTTACAGATT TTATGGATAA ATGTTATAAT AAGAAAAGGA
    AATAATATAA TCATCTATGC ATGGAATAAT TGTAGTGGGA ACTAATAATG
    AGTATGGGTT GTTTTTGGAA GCAGGTGGCA TGTGGCTATG CCCAAAATAT
    TACAACAATC CATCTCCATA CTCTCAGATG CTGGTCTTGG AATGGCTATG
    TTCAGCTTAG GTAAACACTA ACCTTGCTTC ATGCAAATCT AGACTTGTTT
    TGATGATGAT CTAATCTTTA AAGTTGGGTC CAAATTTGTT CTCATCTTAT
    CTGTTAAAAA AGATTCATTT AATGCAAAGT GGGTCTGTGT GTTTAATTAG
    TCGTAGTTGT CCAGCTTGTA ATTGGTAAAA GTCATATAAA CTGTACAAAG
    CACTTACCCA AAGAGAGAAT CCTGACCCTC TGACCCAGAC ATGTTGGTGT
    GTCCGGTCCC CACGCCGAGT CCCCACTGGC TCTGTCTACT TATTTGGGTC
    TCACCAAGTA GATGACCTAA CTCTTTGCTG TCAATTTTCT CAAATCCTTT
    TAGTTAACGA TTGCTTATTT TT
  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 181663 [Map]; Strand: w
      • AT2G01420.1 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon2 (CDS)
      • AT2G01420.2 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: GT16272 - 3-prime edge
    AAACGGTAGA GGATTTTACC GACCGTTACC GACCGTTTTC ATCCATAATA 
    TTGATAATGG TGTGGAGAAA GCTGATCAGA AACCCAAACA CGTACTCCAG
    TCTAATCGGT CTCATATGGG CTCTTGTTGC TTACCGGTCA GTGAGTCCAA
    TCATTTTCTT ACTTACAGAT TTTATGGATA AATGTTATAA TAAGAAAAGG
    AAATAATATA ATCATCTATG CATGGAATAA TTGTAGTGGG AACTAATAAT
    GAGTATGGGT TGTTTTTGGA AGCAGGTGGC ATGTGGCTAT GCCCAAAATA
    TTACAACAAT CCATCTCCAT ACTCTCAGAT GCTGGTCTTG GAATGGCTAT
    GTTCAGCTTA GGTAAACACT AACCTTGCTT CATGCAAATC TAGACTTGTT
    TTGATGATGA TCTAATCTTT AAAGTTGGGT CCAAATTTGT TCTCATCTTA
    TCTGTTAAAA AAGATTCATT TAATGCAAAG TGGGTCTGTG TGTTTAATTA
    GTCGTAGTTG TCCAGCTTGT AATTGGTAAA AGTCATATAA ACTGTACAAA
    GCACTTACCC AAAGAGAGAA TCCTGACCCT CTGACCCAGA CATGTTGGTG
    TGTCCGGTCC CCACGCCGAG TCCCCACTGG CTCTGTCTAC TTATTTGGGT
    CTCACCAAGT AGATGACCTA ACTCTTTGCT GTCAATTTTC TCAAATCCTT
    TTAGTTA

AT2G01420.1

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a putative auxin efflux carrier that is localized in developing and mature root meristems. It is involved in the maintenance of embryonic auxin gradients. A role for AtPIN4 in generating a sink for auxin below the quiescent center of the root meristem that is essential for auxin distribution and patterning is proposed. In the root, PIN4 is detected around the quiescent center and cells surrounding it, and localizes basally in provascular cells. PIN4 expression is upregulated in brassinosteroid-insensitive mutant (PMID 16141452).
Computational Description
PIN-FORMED 4 (PIN4); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity; INVOLVED IN: root development, pattern specification process, auxin polar transport; LOCATED IN: endomembrane system, integral to membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1811 Blast hits to 1602 proteins in 498 species: Archae - 31; Bacteria - 1046; Metazoa - 12; Fungi - 10; Plants - 485; Viruses - 0; Other Eukaryotes - 227 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G01420.2

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a putative auxin efflux carrier that is localized in developing and mature root meristems. It is involved in the maintenance of embryonic auxin gradients. A role for AtPIN4 in generating a sink for auxin below the quiescent center of the root meristem that is essential for auxin distribution and patterning is proposed. In the root, PIN4 is detected around the quiescent center and cells surrounding it, and localizes basally in provascular cells. PIN4 expression is upregulated in brassinosteroid-insensitive mutant (PMID 16141452).
Computational Description
PIN-FORMED 4 (PIN4); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity; INVOLVED IN: root development, pattern specification process, auxin polar transport; LOCATED IN: endomembrane system, integral to membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1823 Blast hits to 1616 proteins in 502 species: Archae - 31; Bacteria - 1046; Metazoa - 18; Fungi - 10; Plants - 487; Viruses - 2; Other Eukaryotes - 229 (source: NCBI BLink).
Link
InterPro Scan - TAIR