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GT16494

CSHL Arabidopsis genetrap mutant lines

Original: GT16494

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT16494

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 8705002 [Map]; Strand: c
      • AT2G20180.1 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon7 (CDS)
      • AT2G20180.2 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon6 (CDS)
      • AT2G20180.3 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon7 (CDS)
    Flanking Genome Sequence: GT16494 - 3-prime edge
    GGTAGAGTAT TTTACCGACC GTTACCGACC GTTTTCATCC CTAATCATTG 
    GCATCATTCC ACATCCCATT GACATCATCT GCATTAATCT CAGTCATATA
    AACTGGAGTT TTCATGGTAA AGTATACATT TTCAAGAACT TAAAAAGGGC
    TTTCAAGACT TAGAATCCTT TAAAGAAAAT GAACCTGTAT TTGAAGCTGA
    AGAGATTTCA TGTACTCAAT AGCTTCATCT AACATCGAAG CTTTATCTGA
    CTGAGGATCA AAGAAACAAC AATCAGTAAC AAGGTTAACG TATACAATAA
    CTTGAAGAGA AAGAAAAAAG ATCGTCGAAG ACCTTGTTGC AGCGAGGTAT
    AAGTTCTTGC AAAGATTTCA TTCT
  • 5 prime edge [+] show sequence

    1. Chromosome: 2; Position: 8704880 [Map]; Strand: c
      • AT2G20180.1 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon7 (CDS)
      • AT2G20180.2 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon6 (CDS)
      • AT2G20180.3 - [+] show detail - phytochrome interacting factor 3-like 5
        Distance: 0; Region: exon7 (CDS)
    Flanking Genome Sequence: GT16494 - 5-prime edge
    GTATGGGTAT GAACCAGCCT ATTCCTCCTC CTTCCTTCAT GCCATTCCCC 
    AACATGTTAG CCGCTCAAAG ACCTTTGCCT ACACAAACTC ACATGGCCGG
    GTCAGGACCG CAATACCCTG TTCATGCTTC TGACCCGTCA AGAGTCTTTG
    TACCGAACCA GCAGTATGAT CCAACCTCGG GCCAGCCTCA GTATCCAGCT
    GGTTACATGG ATCCATATCA GCAGTTCCGC GGTCTCCACC CGACCCAACC
    ACCTCAGTTT CAGGTACTAA ACAGTCATCT CTTTCCCAAA AATGGCTAGT
    TCCTCTGCTT ACAAGTCACA AGTTCTTATC TTTCTTATTT TAGAATCAAG
    CAACATCGTA CCCAAGTTCG AGCAGGGTGA GTAGTAGTAA GGAATCTGAG
    GATCACGGAA ACCACACAAC AGGTTAATAA TGTCCATGGA GCAACAAGAA
    GATCTGTTTT CACAAGCAAA CACAATTTTG AGAAATTGAC AGAGAGACCT
    AACATGTATA TATATCGCCA TCTGTTTCTT GTTTTTCTTT GGTTTGTTTT
    GTCCTCTCTT CTCAGGTTGT ATACTTAGAG AGCTGTACAT GTAATGATCC
    AGAGATCTAG GAATCAATAC ATAGAGGTTG CAGAGGCTTA GTGCAAAGAA
    TAGATATGAG AAAGTTAGGT TCATATACAT ACCCGATCAT A

AT2G20180.1

Model type
Protein coding
Short Description
phytochrome interacting factor 3-like 5
Curator Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
Computational Description
phytochrome interacting factor 3-like 5 (PIL5); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT4G28800.1); Has 4635 Blast hits to 4615 proteins in 348 species: Archae - 0; Bacteria - 26; Metazoa - 878; Fungi - 251; Plants - 3376; Viruses - 2; Other Eukaryotes - 102 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G20180.2

Model type
Protein coding
Short Description
phytochrome interacting factor 3-like 5
Curator Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
Computational Description
phytochrome interacting factor 3-like 5 (PIL5); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT4G28800.1); Has 4736 Blast hits to 4693 proteins in 351 species: Archae - 0; Bacteria - 27; Metazoa - 921; Fungi - 265; Plants - 3401; Viruses - 2; Other Eukaryotes - 120 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G20180.3

Model type
Protein coding
Short Description
phytochrome interacting factor 3-like 5
Curator Summary
Encodes a novel Myc-related bHLH transcription factor that has transcriptional activation activity in the dark. It is a key negative regulator of phytochrome-mediated seed germination and acts by inhibiting chlorophyll biosynthesis, light-mediated suppression of hypocotyl elongation and far-red light-mediated suppression of seed germination, and promoting negative gravitropism in hypocotyls. Light reduces this activity in a phy-dependent manner. The protein preferentially interacts with the Pfr forms of Phytochrome A (PhyA) and Phytochrome B (PhyB), is physically associated with APRR1/TOC1 and is degraded in red (R) and far-red (FR) light through the ubiquitin (ub)-26S proteasome pathway to optimize photomorphogenic development in Arabidopsis. It also negatively regulates GA3 oxidase expression.
Computational Description
phytochrome interacting factor 3-like 5 (PIL5); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT4G28800.1).
Link
InterPro Scan - TAIR