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GT17225

CSHL Arabidopsis genetrap mutant lines

Original: GT17225

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT17225

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 1579633 [Map]; Strand: w
      • AT4G03550.1 - [+] show detail - glucan synthase-like 5
        Distance: 0; Region: exon5 (3UTR)
      • AT4G03560.1 - [+] show detail - two-pore channel 1
        Distance: -423; Region: promoter
    Flanking Genome Sequence: GT17225 - 5-prime edge
    TTGTTCTAAA GGTGCAGCAA TGTGTATTGA GGCTTAGAGT ATAAGATCTC 
    CTTCTCTCAT TATAGAGCTT TAGAGCATTC TTAGTTTATA TTCAGATTGT
    TATCTAAAGT CATCTCGCAG AGCTTTTGTT CGTATTTCAC ATCTTTTCTT
    CTCC

AT4G03550.1

Model type
Protein coding
Short Description
glucan synthase-like 5
Curator Summary
Encodes a callose synthase that is required for wound and papillary callose formation in response to fungal pathogens Erysiphe and Blumeria. Mutants are resistant to P. parasitica and exhibit an exaggerated PR1 response.Contributes to PAMP-induced basal defense.
Computational Description
glucan synthase-like 5 (GSL05); FUNCTIONS IN: transferase activity, transferring glycosyl groups, 1,3-beta-glucan synthase activity; INVOLVED IN: in 12 processes; LOCATED IN: 1,3-beta-glucan synthase complex, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl transferase, family 48 (InterPro:IPR003440); BEST Arabidopsis thaliana protein match is: glucan synthase-like 1 (TAIR:AT4G04970.1); Has 1221 Blast hits to 894 proteins in 155 species: Archae - 2; Bacteria - 0; Metazoa - 0; Fungi - 695; Plants - 448; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G03560.1

Model type
Protein coding
Short Description
two-pore channel 1
Curator Summary
Encodes a depolarization-activated Ca(2+) channel. Anti-sense experiments with this gene as well as Sucrose-H(+) symporters and complementation of yeast sucrose uptake mutant cch1 suggest that this protein mediates a voltage-activated Ca(2+ )influx. Mutants lack detectable SV channel activity suggesting TPC1 is essential component of the SV channel. Patch clamp analysis of loss of function mutation indicates TPC1 does not affect Ca2+ signaling in response to abiotic and biotic stress.
Computational Description
two-pore channel 1 (TPC1); FUNCTIONS IN: voltage-gated calcium channel activity, calcium channel activity; INVOLVED IN: regulation of jasmonic acid biosynthetic process, calcium ion transport, calcium-mediated signaling, seed germination, regulation of stomatal movement; LOCATED IN: vacuolar membrane, plasma membrane, vacuole, plant-type vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: EF-HAND 2 (InterPro:IPR018249), Ion transport (InterPro:IPR005821), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR