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GT18508

CSHL Arabidopsis genetrap mutant lines

Original: GT18508

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT18508

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 17217901 [Map]; Strand: c
      • AT4G36480.1 - [+] show detail - long-chain base1
        Distance: -231; Region: promoter
      • AT4G36480.2 - [+] show detail - long-chain base1
        Distance: -466; Region: promoter
    Flanking Genome Sequence: GT18508 - 3-prime edge 
    CTTCCTTACC TTTAGGCTTT AGTAGTCTTT CTTTCTCTCT TTGCATTGTC 
    CTTTAAGACT TTAGTGGTGG TGATGAATGC TTACTGAGTC ATATAAGTGT 
    AGTTTATATG CTTTGTTGGG AAAGAGAGAG AGTTTGATAG TTTTGGTACG 
    AAATGGATTT GTGTTTAGAG GGGAACTCAC GCGTGTCCTT TGAGGGATTA 
    ATGCGAAGCC GTAACGTATC AATCTTATTC TGTGGTTGGA GTTCTCATAA 
    ACGTTCATAT AATCTCTTTT TTAACCATGA TCTTCTCTCT ATTGTCTTGG 
    AATTTGTTTG CTCTGTGTGT GTG

AT4G36480.1

Model type
Protein coding
Short Description
long-chain base1
Curator Summary
Encodes the LCB1 subunit of serine palmitoyltransferase. Together with the LCB2 subunit, forms a functional serine palmitoyltransferase complex, which catalyzes the first reaction of sphingolipid biosynthesis. Knockout of LCB1 was embryo lethal. Partial suppression of LCB1 expression led to smaller plants due to reduced cell expansion.
Computational Description
long-chain base1 (LCB1); FUNCTIONS IN: protein binding, serine C-palmitoyltransferase activity; INVOLVED IN: cell growth, sphingolipid biosynthetic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G36480.2

Model type
Protein coding
Short Description
long-chain base1
Curator Summary
Encodes the LCB1 subunit of serine palmitoyltransferase. Together with the LCB2 subunit, forms a functional serine palmitoyltransferase complex, which catalyzes the first reaction of sphingolipid biosynthesis. Knockout of LCB1 was embryo lethal. Partial suppression of LCB1 expression led to smaller plants due to reduced cell expansion.
Computational Description
long-chain base1 (LCB1); FUNCTIONS IN: protein binding, serine C-palmitoyltransferase activity; INVOLVED IN: cell growth, sphingolipid biosynthetic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR