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GT20458

CSHL Arabidopsis genetrap mutant lines

Original: GT20458

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT20458

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26623326 [Map]; Strand: c
      • AT5G66680.1 - [+] show detail - dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit f...
        Distance: -2659; Region: intergenic
      • AT5G66690.1 - [+] show detail - UDP-Glycosyltransferase superfamily protein
        Distance: -1754; Region: intergenic
    Flanking Genome Sequence: GT20458 - 5-prime edge 
    CGTATACAAC TCAATATTAT GATCATGTAT TTCATAAAAT ATCTTCTGAG 
    GAATCTATAG AAAAATGACC AATTAATCAC CACATGATCG GGCTCACGTT 
    GGAGATGGTG GATCACATGC CCGATATTAT ATTTTATGTA AATCAGTAAT 
    TAATATTGAC CAGCACATAT TCCAATGGTG AAAAGAATAT GTATACACAT 
    TCAGACATTC TATCGAAAAA GGAAGAATAA AAACAAAAAG TGAGGAGAAA 
    AGAAAGATAG TAACTGTTTT GATTCTATGA TGAAAGTAC

AT5G66680.1

Model type
Protein coding
Short Description
dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit family protein
Curator Summary
Encodes a protein ortholog of human SOT48 or yeast WBP1, an essential protein subunit of the oligosaccharyltransferase (OST) complex, which is responsible for the transfer in the ER of the N-linked glycan precursor onto Asn residues of candidate proteins.
Computational Description
DEFECTIVE GLYCOSYLATION (DGL1); FUNCTIONS IN: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; INVOLVED IN: plant-type cell wall organization, protein amino acid N-linked glycosylation via asparagine, unidimensional cell growth; LOCATED IN: in 8 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit (InterPro:IPR005013); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G66690.1

Model type
Protein coding
Short Description
UDP-Glycosyltransferase superfamily protein
Curator Summary
UGT72E2 is an UDPG:coniferyl alcohol glucosyltransferase which glucosylates sinapyl- and coniferyl aldehydes as well as sinapyl- and coniferyl alcohol. The enzyme is thought to be involved in lignin metabolism. A knockdown mutant line (72E2KD) was obtained using RNAi silencing. A twofold reduction in coniferyl alcohol 4-O-glucoside and sinapyl alcohol 4-O-glucoside was detected in this line compared to wildtype. In comparison, both knockout and knockdown lines of UGT72E1 and UGT72E3, respectively, failed to display the same reduction in phenylpropanoid 4-O-glucosides.
Computational Description
UGT72E2; CONTAINS InterPro DOMAIN/s: UDP-glucuronosyl/UDP-glucosyltransferase (InterPro:IPR002213); BEST Arabidopsis thaliana protein match is: UDP-Glycosyltransferase superfamily protein (TAIR:AT5G26310.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR