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GT22263

CSHL Arabidopsis genetrap mutant lines

Original: GT22263

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT22263

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 9832925 [Map]; Strand: c
      • AT3G26744.1 - [+] show detail - basic helix-loop-helix (bHLH) DNA-binding superfamily protein
        Distance: 0; Region: exon4 (3UTR)
      • AT3G26744.2 - [+] show detail - basic helix-loop-helix (bHLH) DNA-binding superfamily protein
        Distance: 0; Region: exon5 (3UTR)
      • AT3G26744.4 - [+] show detail - basic helix-loop-helix (bHLH) DNA-binding superfamily protein
        Distance: 0; Region: exon5 (3UTR)
    Flanking Genome Sequence: GT22263 - 5-prime edge 
    TATCCCTGCT TTTCGATACA GCAGGGTATG CTGGTATGAT CTGATCTGAT 
    CCTGACTTCG AGTCCATTAA GCATCTGTTG AAGCAGAGCT AGAAGAACTA 
    AGTCCCTTTA AATCTGCAAT TTTCTTCTCA CTCGACTATC TTATGTCATA 
    ACTTCAATCT AAGCATGTAA TGCAATTGCA AATGAGAGTT GTTTTTAAAT 
    TAAGCTTTTG AGAACTTGAG GTTGTTGTTG TTGGATACAT AACTTCAACC 
    TTTTATTAGC AATGTTAACT TCCATTTATG TTTCATCTTA AAGCTATGCT 
    CAAGAATTTT TTTTAAAGTC TTCATCTTTG TTCATGAGTG AACTTCTCAA 
    AGAAGACAGA CAAAACATTT GCACATATAC AGACAAAACA GTTATGTTAA 
    AGGTATAGCT TTGGTATTAG CTGATGAAAT GAGCTTTGAT GATGTAAGAG 
    TGAAGAAAAA CATTGAGATT TTTAAGAAAA AACAGAGAAA AGAATGGTGA 
    AGGAAGAGGC AGAGCAAAGA ATAGACCCTA CCATTTTGTT TGTTTTCTTC 
    TTT

AT3G26744.1

Model type
Protein coding
Short Description
basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Curator Summary
Encodes a MYC-like bHLH transcriptional activator that binds specifically to the MYC recognition sequences in the CBF3 promoter. Mutants are defective in cold-regulated gene expression. Cold stress triggers protein degradation of nuclear GFPICE1 protein, and the RING finger protein HOS1 is required. Sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance.
Computational Description
INDUCER OF CBF EXPRESSION 1 (ICE1); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G12860.1); Has 2623 Blast hits to 2616 proteins in 144 species: Archae - 0; Bacteria - 0; Metazoa - 19; Fungi - 30; Plants - 2572; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G26744.2

Model type
Protein coding
Short Description
basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Curator Summary
Encodes a MYC-like bHLH transcriptional activator that binds specifically to the MYC recognition sequences in the CBF3 promoter. Mutants are defective in cold-regulated gene expression. Cold stress triggers protein degradation of nuclear GFPICE1 protein, and the RING finger protein HOS1 is required. Sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance.
Computational Description
INDUCER OF CBF EXPRESSION 1 (ICE1); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G12860.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G26744.4

Model type
Protein coding
Short Description
basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Curator Summary
Encodes a MYC-like bHLH transcriptional activator that binds specifically to the MYC recognition sequences in the CBF3 promoter. Mutants are defective in cold-regulated gene expression. Cold stress triggers protein degradation of nuclear GFPICE1 protein, and the RING finger protein HOS1 is required. Sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance.
Computational Description
INDUCER OF CBF EXPRESSION 1 (ICE1); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G12860.1); Has 2623 Blast hits to 2616 proteins in 144 species: Archae - 0; Bacteria - 0; Metazoa - 19; Fungi - 30; Plants - 2572; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink).
Link
InterPro Scan - TAIR