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GT22971

CSHL Arabidopsis genetrap mutant lines

Original: GT22971

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT22971

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 19160968 [Map]; Strand: c
      • AT1G51680.1 - [+] show detail - 4-coumarate:CoA ligase 1
        Distance: 0; Region: exon1 (CDS)
      • AT1G51680.3 - [+] show detail - 4-coumarate:CoA ligase 1
        Distance: 0; Region: exon1 (CDS)
      • AT1G51680.2 - [+] show detail - 4-coumarate:CoA ligase 1
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT22971 - 5-prime edge
    CACCGAAGCT CGTTACGTCG ACAAAATCAA ACCACTTCAA AACGACGACG 
    GAGTAGTCAT CGTCTGCATC GACGACAACG AATCCGTGCC AATCCCTGAA
    GGCTGCCTCC GCTTCACCGA GTTGACTCAG TCGACAACCG AGGCATCAGA
    AGTCATCGAC TCGGTGGAGA TTTCACCGGA CGACGTGGTG GCACTACCTT
    ACTCCTCTGG CACGACGGGA TTACCAAAAG GAGTGATGCT GACTCACAAG
    GGACTAGTCA CGAGCGTTGC TCAGCAAGTC GACGGCGAGA ACCCGAATCT
    TTATTTCCAC AGCGATGACG TCATACTCTG TGTTTTGCCC ATGTTTCATC
    T

AT1G51680.1

Model type
Protein coding
Short Description
4-coumarate:CoA ligase 1
Curator Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.
Computational Description
4-coumarate:CoA ligase 1 (4CL1); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 83980 Blast hits to 76655 proteins in 3756 species: Archae - 1185; Bacteria - 53552; Metazoa - 3476; Fungi - 4550; Plants - 2810; Viruses - 1; Other Eukaryotes - 18406 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G51680.2

Model type
Protein coding
Short Description
4-coumarate:CoA ligase 1
Curator Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.
Computational Description
4-coumarate:CoA ligase 1 (4CL1); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 80831 Blast hits to 73675 proteins in 3786 species: Archae - 1165; Bacteria - 53622; Metazoa - 3406; Fungi - 4376; Plants - 2578; Viruses - 1; Other Eukaryotes - 15683 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G51680.3

Model type
Protein coding
Short Description
4-coumarate:CoA ligase 1
Curator Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.
Computational Description
4-coumarate:CoA ligase 1 (4CL1); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 83069 Blast hits to 75791 proteins in 3772 species: Archae - 1181; Bacteria - 53827; Metazoa - 3457; Fungi - 4480; Plants - 2791; Viruses - 1; Other Eukaryotes - 17332 (source: NCBI BLink).
Link
InterPro Scan - TAIR