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GT23974

CSHL Arabidopsis genetrap mutant lines

Original: GT23974

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT23974

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 23452716 [Map]; Strand: w
      • AT3G63510.1 - [+] show detail - FMN-linked oxidoreductases superfamily protein
        Distance: 0; Region: exon12 (3UTR)
      • AT3G63510.2 - [+] show detail - FMN-linked oxidoreductases superfamily protein
        Distance: 0; Region: exon12 (3UTR)
      • AT3G63520.1 - [+] show detail - carotenoid cleavage dioxygenase 1
        Distance: -195; Region: promoter
    Flanking Genome Sequence: GT23974 - 5-prime edge 
    CATACGAAGC TGGAGAAGAA TCATCTTATG CGCTTAGTTG TCGTTTGTAA 
    TCTGTGTTTG TGATTGTCTC TGTCGATTAA AATTGTAAAT TGAACGGGGC 
    TTACTTCAAG TGGTTGTTAG TCTTGGGCTC GTGTATGGGC TTTTTTGTTA 
    TTCTTTTTGT TTGGGCCGGA AATATAACTG GGTCCATCAT CTCAAGAAAA 
    AAGATTCCGT TGCGACGTGG CAGCGTTTCC GCCAAGACGC GGGCCATATT 
    ATCTGGTCTC AAATCACCTC CTCCTTGCAG CAAAACAAAC AATCATGGCG 
    GAGAAACTCA GTGATGGCAG CATCATCATC TCAGTCCATC CTAGACCCTC 
    CAAGGGTTTC TCCTCGAAGC TTCTCGATCT TCTCGAGAGA CTTGTTGTCA 
    AGCTCATGCA CGATGCTTCT CTCCCTCTCC ACTACCTCTC AGGCAACTTC 
    GCTCCCATCC GTGATGAAAC TCCTCCCGTC AAGGATCTCC CCGTCCATGG 
    ATTTCTTCCC GTGAAATTCT CTCATATTCT CTTTGATCCT TTCCCCTAAG 
    GGTTATTTAG TATTCTTATG

AT3G63510.1

Model type
Protein coding
Short Description
FMN-linked oxidoreductases superfamily protein
Computational Description
FMN-linked oxidoreductases superfamily protein; FUNCTIONS IN: tRNA dihydrouridine synthase activity, FAD binding, catalytic activity; INVOLVED IN: regulation of nitrogen utilization, tRNA processing, oxidation reduction, metabolic process; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), tRNA-dihydrouridine synthase (InterPro:IPR001269); BEST Arabidopsis thaliana protein match is: Aldolase-type TIM barrel family protein (TAIR:AT5G47970.2); Has 11920 Blast hits to 11916 proteins in 2583 species: Archae - 58; Bacteria - 8071; Metazoa - 278; Fungi - 218; Plants - 160; Viruses - 0; Other Eukaryotes - 3135 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G63510.2

Model type
Protein coding
Short Description
FMN-linked oxidoreductases superfamily protein
Computational Description
FMN-linked oxidoreductases superfamily protein; FUNCTIONS IN: tRNA dihydrouridine synthase activity, FAD binding, catalytic activity; INVOLVED IN: regulation of nitrogen utilization, tRNA processing, oxidation reduction, metabolic process; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), tRNA-dihydrouridine synthase (InterPro:IPR001269); BEST Arabidopsis thaliana protein match is: Aldolase-type TIM barrel family protein (TAIR:AT5G47970.2); Has 11664 Blast hits to 11660 proteins in 2553 species: Archae - 58; Bacteria - 8007; Metazoa - 234; Fungi - 152; Plants - 146; Viruses - 0; Other Eukaryotes - 3067 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G63520.1

Model type
Protein coding
Short Description
carotenoid cleavage dioxygenase 1
Curator Summary
Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.
Computational Description
carotenoid cleavage dioxygenase 1 (CCD1); FUNCTIONS IN: 9-cis-epoxycarotenoid dioxygenase activity; INVOLVED IN: response to water deprivation, carotene catabolic process, xanthophyll catabolic process, carotenoid catabolic process; LOCATED IN: plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Carotenoid oxygenase (InterPro:IPR004294); BEST Arabidopsis thaliana protein match is: nine-cis-epoxycarotenoid dioxygenase 4 (TAIR:AT4G19170.1); Has 2926 Blast hits to 2896 proteins in 500 species: Archae - 16; Bacteria - 808; Metazoa - 317; Fungi - 194; Plants - 866; Viruses - 0; Other Eukaryotes - 725 (source: NCBI BLink).
Link
InterPro Scan - TAIR