GT6081

CSHL Arabidopsis genetrap mutant lines

Phenotypes

Morphological phenotypes

Original description

dead or tiny plants

Anatomical entity /structure development stage	Phenotypes
whole plant (PO:0000003)	decreased height (PATO:0000569)

Original: GT6081

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

3 prime edge [+] show sequence

Chromosome: 3; Position: 1605934 [Map]; Strand: w
- AT3G05530.1 - [+] show detail - regulatory particle triple-A ATPase 5A
  Distance: 0; Region: exon10 (CDS)

TTACCGAGCG TTACCGACCG TTTTCATCCC TACACCTGAG ATTCAACAAA 
ACAGTATTAC TATATCTCGC AGCGAATAAA CGCTAAACGG TTTTCCCATA 
ACATCTTCAA AAAATATGAA AAGGTGATGC AGGACTTATG TTGATAAAGA 
GGTTCTGTTG TCTTTTTTAC CTCTGTGGCA TCACGGCGAA AAGCTAACAT 
GCCTGCCTCT ACACACACTG CTTTCAGCTG AGCTCCATTG AAATCATCTG 
TTGATCGTGC AAGCTCCTCA AAGTTGACGT CTGGGTGGAC ATTCATCTCC 
CTCGACTAAA TCTGGATTAA TTC

5 prime edge [+] show sequence
1. Chromosome: 3; Position: 3512803 [Map]; Strand: c
  - AT3G11210.1 - [+] show detail - SGNH hydrolase-type esterase superfamily protein
    Distance: 0; Region: exon1 (CDS)
Flanking Genome Sequence: GT6081 - 5-prime edge
```
TCGTTTTGTT TGGATCTTCC ATTGTTCAGA TGAGCTTTGG CCATGGTGGT 
TGGGGCGCCA TTCTCTCCGA GGTCTACGCT CGTATGGTAT CGTCAGATTC 
ATCAATCTCT CTCTCTCTGT GGCTTCTGGA TTCGATA
```

AT3G05530.1

Model type: Protein coding
Short Description: regulatory particle triple-A ATPase 5A
Curator Summary: Encodes RPT5a (Regulatory Particle 5a), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.
Computational Description: regulatory particle triple-A ATPase 5A (RPT5A); CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: 26S proteasome AAA-ATPase subunit RPT5B (TAIR:AT1G09100.1); Has 33265 Blast hits to 30927 proteins in 3145 species: Archae - 1437; Bacteria - 12044; Metazoa - 4947; Fungi - 3672; Plants - 3322; Viruses - 56; Other Eukaryotes - 7787 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G11210.1

Model type: Protein coding
Short Description: SGNH hydrolase-type esterase superfamily protein
Computational Description: SGNH hydrolase-type esterase superfamily protein; FUNCTIONS IN: hydrolase activity, hydrolase activity, acting on ester bonds, carboxylesterase activity; INVOLVED IN: lipid metabolic process; LOCATED IN: cellular_component unknown; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Esterase, SGNH hydrolase-type, subgroup (InterPro:IPR013831), Lipase, GDSL (InterPro:IPR001087), Esterase, SGNH hydrolase-type (InterPro:IPR013830); BEST Arabidopsis thaliana protein match is: SGNH hydrolase-type esterase superfamily protein (TAIR:AT2G38180.1); Has 768 Blast hits to 767 proteins in 294 species: Archae - 0; Bacteria - 304; Metazoa - 76; Fungi - 152; Plants - 166; Viruses - 0; Other Eukaryotes - 70 (source: NCBI BLink).
Link: InterPro Scan - TAIR