GT6235

CSHL Arabidopsis genetrap mutant lines

Phenotypes

Morphological phenotypes

Original description

tiny, chlorotic plants
embryo lethal

Anatomical entity /structure development stage	Phenotypes
whole plant (PO:0000003)	decreased height (PATO:0000569)
seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997)

Original: GT6235

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

3 prime edge [+] show sequence

Chromosome: 3; Position: 17483623 [Map]; Strand: c
- AT3G47450.1 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
  Distance: 0; Region: exon12 (CDS)
- AT3G47450.2 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
  Distance: 0; Region: exon12 (CDS)

AATTTGNCCN ATTNGAGGCC GTTTTAACCG NGNCCCACCG AGNGCNTTCA 
TCCANCCAGT TCTTTCTGCA GAACAAATGA GTATCAAGTA AATGGTAATA 
AGAANATTTA ACTTGTCCTT AAATAAGTAA CGAAGTTGAA AATGATGGGG 
AACGGAACTT GCCTCGTAAA AGGCAGTCGC TGTTTTGGTT GGTACTGCAT 
GAATCTCAAG AGCTTNTGGT CCATANAATG TGAAACATGT TNCCGGTANA 
NCCTGCAATA TGTCAACTAT GTNAAAAAAT TCTGATATCC ACTGTGAAAC 
TGAAACATAA TTCTANTAAT AATAGTTATT ACCTTCAAGA TGTCANTCCT 
AACGAGACCT CCCCANAAAA ATGTATAACC GTTTAAGCTC TCACCCTTGG 
GACTACTTGA CGATTGAGTT GGCAAAGTTG AAATCTGTAA GGAACAAAAA 
GCACAGGAAG ATCATCACAC TGGTGTTACG GTAAAATAAT CTTTACCTGG 
CCGTAATAAA GCANACTTAC ATCNAAAAGA TTGGCCTCTG ANACCATTTN 
GAGGAGCAAG GGCGGGGTNA ATCATCTGAA TGAACNACAG CTGCTTGCCT 
GTGGTGTAGG TGCCNCACCC NGTGTGTCNT ACAACTTCCT GTGGTAATAA 
ATGTATTCTT TTTAACAAAA TGTCTACTAT TGCAATTTTT GGGANAGTTC 
CTACAATCNG AACTTGGGTT TAAGTTTATA CCTCTCCCCC GAAGGAAAAG 
CCGTTGATCT GGATTTGGAC CTANCNGTGG TTTCCCTTGG GAACNGCCNN 
AATTGAANTT GGGTTTNGTT CTTTTTGTTG CCCNCCCGCN GGCCCCCANG 
GATCCCCTTT TTCGGCCCN

3 prime edge [+] show sequence
1. Chromosome: 3; Position: 17483894 [Map]; Strand: c
  - AT3G47450.1 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
    Distance: 0; Region: intron10 (CDS)
  - AT3G47450.2 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
    Distance: 0; Region: intron10 (CDS)
Flanking Genome Sequence: GT6235 - 3-prime edge
```
TGCTCTTCGT CCTATTAATT GCGATATCCT TCTTTCGCTG CCTCTTCTCT 
CTCGACGATA CCTTCAAGAT GTCAATCCTA ACGAGACCTC CCCAGAAAAA 
TGTATAACCG TTTAAGCTCT CACCCTTGGG ACTACTTGAC GATTGAGTTG 
GCAAAGTTGA AATCTGTAAG GAACAAAAAG CACAGGAAGA TCATC
```

5 prime edge [+] show sequence

Chromosome: 3; Position: 17483557 [Map]; Strand: c
- AT3G47450.1 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
  Distance: 0; Region: exon12 (CDS)
- AT3G47450.2 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
  Distance: 0; Region: exon12 (CDS)

AGGNTCCNNA ANTNNCTCCT CCATCAGGGG AAANAATCAG ATGCAGGAGT 
GGAAAGGGTT ACAATCTCAC CGGTTACTTC AAATCGAAAT CAACGATGCA 
AAAAGGTGAA ATATATATCT TCCATTCCCA TCTATGTTTA GTAAAACAAA 
TGATCCTCAC CTAACGTTAA CACACCTCTT GATCAGACCG GCTAGTGATG 
TGGCAATATC AGGGTTAGGA TGGATTTCAA TTGAACCAAT CCGCAAAACA 
CGAGGAACTG AACCGAGAGA TCTCAATGAA NCANAGCATG AGATACATAT 
TTGTGTCAGT GTGCCAAAAC CAGTTGAAGT TTTTCTTCGA CCAACATTGC 
CAATTGGTAC TTCAGGTACT GAATGGTATC AGTATCGTGA GTTAACCGAT 
AAGGAAGAAN AAGTAAGACC CAAATGGTAC TTTTGAATTT TTTTTGTTCA 
NATGCNAAAA TNTCGGCTTA GTATCNCNAA CCANAGTTNT ATGTAACANA 
ATANTNAATT GTTTGTAATG AANNCTACTT AAGTAACATA CCNNTCAAGG 
AATTAAACTC TANTCTACNA CATTTTCNGA NACTGAAAAT TANNAAAGAT 
CNCCNTCNCC CATTCNNCAA ATGAANCAGT AAAAACAANT CTTTACACNC 
CAATGGCATC ACCNCTNNAT NACCCNCCAN CNNCTTCTNC ANGGACCNCC 
NCCTTTATCT CCCCNNAAGC NNNCTGTGGC CTCCTCCAAG CAGAAGNCCA 
NCCCATTCCC ANCTTGGTTC CTTAANGCTN CCGTAANCAA CCNCCAACCC 
ATAGGCNCCC CCCNGANTCT TGATCCCCCC AANATGGACN CCGCTANAAC 
NTTNGCCCCC NCCCCAAACC CTATAAATAT TCGTCCCNCT GCCAAAANGT 
TCCCCCCTTC CNGGANCCCC CCCCTGAACN CCTTTTATCC CTANANCAAN 
GCTNACCCCC CNCCANAACC ANACCCCC

AT3G47450.1

Model type: Protein coding
Short Description: P-loop containing nucleoside triphosphate hydrolases superfamily protein
Curator Summary: Encodes a protein with similarity to the bacterial YqeH GTPase required for proper ribosome assembly. In Arabidopsis, mutant analyses show that this protein regulates growth and hormonal signaling in plants. It also attenuates oxidative stress and reactive oxygen species (ROS). It also seems to be involved in regulating leaf senescence and cell death. This gene product is also involved in nitric oxide biosynthesis in response to ABA but not exogenous H2O2. This protein also appears to be required for proper plastid biogenesis. Levels of several plastid-localized proteins, including RBCL, ClpP1, and the MEP biosynthesis enzymes DXS and DXR are altered in rif1-1 mutants. This protein was originally characterized as a mitrochondrial-localized nitric oxide synthase, but, the synthase activity was later disproven. In addition, new studies with GFP fusion proteins and chloroplast import assays suggest that this protein is found in chloroplasts.
Computational Description: NO ASSOCIATED 1 (NOA1); FUNCTIONS IN: GTPase activity; INVOLVED IN: in 8 processes; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT3G57180.1); Has 1573 Blast hits to 1527 proteins in 752 species: Archae - 6; Bacteria - 1199; Metazoa - 147; Fungi - 33; Plants - 109; Viruses - 0; Other Eukaryotes - 79 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G47450.2

Model type: Protein coding
Short Description: P-loop containing nucleoside triphosphate hydrolases superfamily protein
Curator Summary: Encodes a protein with similarity to the bacterial YqeH GTPase required for proper ribosome assembly. In Arabidopsis, mutant analyses show that this protein regulates growth and hormonal signaling in plants. It also attenuates oxidative stress and reactive oxygen species (ROS). It also seems to be involved in regulating leaf senescence and cell death. This gene product is also involved in nitric oxide biosynthesis in response to ABA but not exogenous H2O2. This protein also appears to be required for proper plastid biogenesis. Levels of several plastid-localized proteins, including RBCL, ClpP1, and the MEP biosynthesis enzymes DXS and DXR are altered in rif1-1 mutants. This protein was originally characterized as a mitrochondrial-localized nitric oxide synthase, but, the synthase activity was later disproven. In addition, new studies with GFP fusion proteins and chloroplast import assays suggest that this protein is found in chloroplasts.
Computational Description: NO ASSOCIATED 1 (NOA1); FUNCTIONS IN: GTPase activity; INVOLVED IN: in 8 processes; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT3G57180.1); Has 1589 Blast hits to 1531 proteins in 754 species: Archae - 6; Bacteria - 1199; Metazoa - 159; Fungi - 35; Plants - 109; Viruses - 0; Other Eukaryotes - 81 (source: NCBI BLink).
Link: InterPro Scan - TAIR