K00547

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K00547

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1 rosette leaf (PO:0000014)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K00547

Determination of introduced cDNA(s)

  • J013089D14 - Rice Full-length cDNAs - Original: J013089D14

    full reading [+] show sequence

    Nucleotide sequence: J013089D14 - full reading 
    GGCCAGGAGA GGAGTTGGGT TGATTCGCAG CAAAGGCACG GCACGGCACG 
    CACGCACTCG AATTCACCTT CACCTTCAGC GCGAGCCGCG AGCGAGAGAG 
    GAAAAGGGAA GCGACGATCT CTGCCCTTCT AGAAGGGTTT GGGATTTCTT 
    GACTGGACGA CCTCGTGCGA TTTCGGGAGG CAGGAGCCAT GAACGGCAAT 
    AAGCAGTCCC TGATATACAG CTTCGTGGCC AAGGGCTCCG TCGTGCTGGC 
    CGAGCACACC GCCTTCTCCG GCAACTTCAG CACCATCGCC GTCCAGTGCC 
    TCCAGAAGCT TCCCCCCAAC ACCTCCAAGT CCACCTACTC CTGCGATGGC 
    CACACCTTCA ACTTCCTCGT CGACCGCGGC TTCGTGTTTC TCGTCGTTGC 
    GGATGAGGCC GTTGGAAGGA GCGTGCCGTT TGTGTTCCTA GATAGGGTTA 
    AGGAAGACTT CATGCAGCGG TATGGCTCCA GTATCGACGA GGAGGGTCAA 
    CACCCGCTCG CCGATGATGC AGATGATGAT GACTTCCTCT TGGAGGACCG 
    ATTTAGCATC GCCTACAATC TTGACCGGGA ATTCGGACCG AGGCTGAAGG 
    ATCACATGCT GTATTGTATT AATCATCCAG AGGAAATTAG CAAGCTCTCC 
    AAAGTGAAAG CACACCTCAC GGAGGTCAAA GGGATTATGA TGGACAACAT 
    TGAGAAGATA TTGGAACGTG GAGAGAAGAT TGAGCTTTTG GTCGGCAAGA 
    CTGAAACCTT ACAATCACAG GCTGACAGCT TCCACAGGCA TGGAAGAGAG 
    CTCAGGCGTA AGATGTGGCT TCAGAACCTG AGGTTTAAGT TGATGGTGGG 
    TGGTGCTGTT GCGGCGTTGA TTCTCTTCCT CTGGCTGATT ATTTGCGGGG 
    GCTTCAAGTG CTAGAACATT GAAAGCCTTT TTCTTTGTTT TGCATTCGAT 
    GTGTTTACCG TGTCGTGTGC ATATATAACG ACCAGCAAAT CCTGCTTTCG 
    ATATGCTTCG TGCAGAGTTA AATCTCCTTA TGGACCTTTA TTCCTTGTAG 
    CCTGTTCTCA TGTTTCATAG TATTACAATA GGGGGGCTCT GGAAATGGAA 
    TACTTGTGTA AACAAACCTT GTGAAAGGAG ACAACTTAGC TATCTGTATT 
    ATGTACATTC TGAAATGCTA ATAGCTTATT TCAATCTC

    Gene models with high sequence identity

    1. AT3G54300.2 [+] show detail - vesicle-associated membrane protein 727
      E-value: 3e-98; Score: 355.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Synaptobrevin 4.7e-31
    FPrintScan Synaptobrevin 2.1e-12
    FPrintScan Synaptobrevin 2.1e-12
    FPrintScan Synaptobrevin 2.1e-12
    Gene3D no description 9.4e-23
    PatternScan Synaptobrevin 0
    superfamily Longin-like 6.6e-23
    HMMPanther VAMP-7 1.6e-132
    HMMPanther SNARE PROTEINS 1.6e-132
    ProfileScan Longin 24.29
    ProfileScan Synaptobrevin 18.462

    See the detailed result >

    AT3G54300.2

    Model type
    Protein coding
    Short Description
    vesicle-associated membrane protein 727
    Curator Summary
    Encodes a member of Synaptobrevin -like protein family. VAMP727 is a R-SNARE and interacts with SYP22/VTI11/SYP51. It is required for trafficking of storage proteins to the protein storage vacuoles (PSV) and also for PSV organization and biogenesis. Loss of function mutations have no phenotype but double mutants with SYP22 are embryo lethal.
    Computational Description
    vesicle-associated membrane protein 727 (VAMP727); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: vacuole organization, protein targeting to vacuole; LOCATED IN: endosome, SNARE complex, membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Longin (InterPro:IPR010908), Longin-like (InterPro:IPR011012), Synaptobrevin (InterPro:IPR001388); BEST Arabidopsis thaliana protein match is: vesicle-associated membrane protein 726 (TAIR:AT1G04760.1); Has 2397 Blast hits to 2395 proteins in 262 species: Archae - 0; Bacteria - 0; Metazoa - 970; Fungi - 430; Plants - 618; Viruses - 0; Other Eukaryotes - 379 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR