K00908

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) increased width (PATO:0000600) (Rosette leaf::Shape::wide) upturned (PATO:0002031) (Rosette leaf::Shape::hyponastic) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many) abnormal (PATO:0000460) (Rosette leaf::Others)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	stem (PO:0009047)	decreased thickness (PATO:0000592) (Stem::Shape::thin)
T2	seed (PO:0009010)	decreased width (PATO:0000599) (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K00908

Determination of introduced cDNA(s)

J013108A22 - Rice Full-length cDNAs - Original: J013108A22

full reading [+] show sequence

GATCATCCTC ATCGTCGCGC GCACCACTCG CCCGGTCGCC CCATCCCCAC 
TCTGGCACTC CAGCGCCCAA AAACCTTCGC CTCCGCCTCC GCCATGGGAG 
ACCACCGCGC CACGGCCAAG GTCCGCGTGA ACGGCGCGCC GGCAGAAGCG 
ACGCTCTTCT GCACCGACCC GCCGGGTGCA GCTAATGGCG GCGGCGGAGA 
TGGAGGGGAG CTCCGGTGGC GGTGCGCTGG TGGCGCCGCG GCGGAGCGCG 
TGCTGTCGCT GGACGCGGAC GTGCTCGGGG TCGAGGCCAG GGGTAAGGAG 
GTGGTCGTGA AGGCCTTCGT GCTGCCGGCG GACGCGGCGG CCAGGTCGGT 
GTCCTGCGCC GCGGGTGCGG GGAAGGGAGG AGGAGGACGG AGGAGGCGCA 
GGAGGGATTA CGTGTTCGAG ATGGCCGCCG GCGAGGACGC CGCCGCGGCC 
TGGTGCGACA GGATGAGGGG CTCCCTGGAC TCTCTCGGCC GGCCTAAGAG 
ATTGTTCATC CTGGTCAACC CCTTCGGCGG CAAAAAATGC GGAAAGAAGA 
TTTACGAGGC AGAGATCAAG CCCCTGTTCG AGGCCGCCGG TGTCAACGTC 
ACAATGCAAG AGACCCGGTA TCAGGGGCAC GCCCGGCAGG TGGCGTCTTC 
TCTTGACCTT GCGCGCTACG ATGGCATTGT CTGTGTCAGC GGCGATGGTG 
TCCTCGTGGA GGTAGTAAAT GGAATTCTAC AACGAATGGA TTGGGAGGAA 
GCGATGAAGA TACCGATTGG GGTGGTTCCA GCAGGTACAG GAAATGGCAT 
GGCTAAATCG CTGTTGCATT CTGCCAGTAA GACATACTCG GTTCCAAATG 
CCGTATTTGC CATTATCAGA GGTTACAGTC AGTCCTTGGA TGTATGCACC 
ATTGTGCAGG GAAGGAAGAA ATTTTTCAGT GTCTTGAATA TGACTTGGGG 
TTTGGTGGCT GATATAGATA TTGAGTCTGA GAAGTACAGG TGGATGGGAA 
GTGCACGCTT TGATTTCTAT GCTTTGGTTC GCATAATGAA CTTGAGAAAA 
TACTACGGAA GTATTCAGTA TGTTCCTGCC CCCGGCTATG AGGCATATGG 
AGATGTCGTC AAGCAGGTTG AGAATTGCAC TGTAGAATGC CAAGAACAGA 
TTGGGAAGTC TTTGTGTTCT TATCAAGGTC CTTCCGTTGA GTTTCAAGGT 
TCAGAATGGA GATCCCTTGA TGGTCCATTT GTGTCTATTT GGATCAACAA 
CGTGCAATGG GCTGCTGAGA GTATCATGGC AGCTCCTGGG GCCAAGTTCT 
CAGATGGCTA CATGGATGCA GTCATAGTCA GGGACTGTCC AAAGGCTGAT 
CTTTTAGCCC TACTGATGAA GATGGGCGAT GGGAGTCATG TAAAATCACC 
ATACGTTACG TATCTAAAGG TGAGATGTCT TCGGTTATCA CCAGGCCAGC 
TTGTGGAGAA TCCAAAACGA GGTGGCATAA TAGACGTGGA CGGGGAGGCT 
ATCGCTAGAG GAGAAGGAAC GTACGGCAAA AACCAGAAAC AGGACGTGAT 
GGGTTATGGC CCTTCCATTC AGATGACAGT GCACCGAGCG TTGGCCACAA 
TGTATTGTCC CAAATGACCC CTTTATCTCC TCTCTCTCCC GAGATTGGAG 
TATTTATTCG TGCCAATTGT TTCGCTCTTT GTTAGAAAAA CCTGGTAATC 
TTGAGCAGTA TTCATTAGAA ATATCTTTTG AAAAAAAGAA AACGCTGATA 
CACCATTACA CCTTCGCATG AAACAACATA ACTGGATTAT TTTAACTGG

Gene models with high sequence identity

AT4G21534.1 [+] show detail - Diacylglycerol kinase family protein
E-value: 7e-127; Score: 451.00

InterPro Scan Digest

Program	Description	E-value
HMMPanther	SPHINGOSINE KINASE-RELATED	5.3e-142
HMMPanther	SPHINGOSINE KINASE	5.3e-142
HMMPfam	Diacylglycerol kinase, catalytic domain	9.3e-26
HMMSmart	Diacylglycerol kinase, catalytic domain	2.2e-08
ProfileScan	Diacylglycerol kinase, catalytic domain	39.821

See the detailed result >

AT4G21534.1

Model type: Protein coding
Short Description: Diacylglycerol kinase family protein
Computational Description: Diacylglycerol kinase family protein; FUNCTIONS IN: diacylglycerol kinase activity; INVOLVED IN: activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway; CONTAINS InterPro DOMAIN/s: Diacylglycerol kinase, catalytic domain (InterPro:IPR001206); BEST Arabidopsis thaliana protein match is: sphingosine kinase 1 (TAIR:AT4G21540.1).
Link: InterPro Scan - TAIR

J013107L04 - Rice Full-length cDNAs - Original: J013107L04

full reading [+] show sequence

GATTTCTCTA ACACACGCTA TTCATTCGGC TGTTTTTTTT ACTCATTTTC 
ACCATATTTT GCTCTTTCAA TTTATTTTTT TCGGTTGGTG ATTTATTCCC 
GATTACTCTG TGCCGTGTGT CAATTAACCC TCTCTTTCTA AAAATCGAAC 
TCAAAAAAGA ACGGATTCTA ATCTTTTTTT TTTTTTTGTT AAAATTTTGT 
TTATAATTAT TCTTGTTTAG TTTTAAATTT TATTTGTTTA AGTATCTCTA 
AAGATAATCA AAATGCGTGA ATGTATTTCA ATCCATGTTG GTCAAGCCGG 
TGTTCAAATG GGTAATTCTT GTTGGGAATT ATACTGTTTG GAACACAATA 
TTACACCGGA TGGTACAATG CCAAGTGATA CTACATTAGG TAGTAGTGAT 
GATTCATTTA ACACTTTTTT CTCAGAAACT GGAGCTGGAA GGCATGTGCC 
ACGAGCAGTC TACGTTGATT TAGAACCAAC TGTTGTTGAT GAAGTACGTA 
CTGGAGTCTA TAGACAACTA TTCCATCCTG AGCAATTAAT TACTGGTAAA 
GAAGATGCAG CCAATAATTA TGCAAGAGGT CATTATACAA TTGGCAAAGA 
AATTGTAGAC TTAGTTTTAG ATCGTATTCG CAGGCTTTCA GATCAGTGTA 
CTGGATTGCA AGGTTTCTTG ATTTTTCATT CGTTTGGTGG TGGTACAGGT 
TCTGGTTTCA CCAGTTTATT AATGGAGCGT TTAAGTGTTG ATTATGGTAA 
AAAATCTAAA TTAGAATTCG CTATTTACCC AGCTCCACAA GTTTCAACAG 
CAGTAGTCGA ACCATACAAT AGTATTTTAA CAACACACAC AACTCTAGAA 
CATAGTGATT GTGCGTTTAT GGTAGATAAT GAAGCAATCT ATGATATTTG 
TCGTCGTAAT TTGGATATCG AGAGACCAAC CTACACAAAT TTAAATCGTT 
TAATTGGTCA AATTGTATCA TCTATAACTG CTTCTTTAAG ATTCGATGGT 
GCTTTAAACG TTGATTTAAC TGAATTTCAA ACTAATTTGG TACCTTATCC 
ACGTATTCAT TTCCCTCTTG TCACATATGC ACCTGTCATT TCAGCTGAAA 
AAGCTTATCA TGAACAACTA ACTGTTTCCG AAATAACAAA CTCTTGTTTC 
GAACCTTCCA ATCAAATGGT TAAATGTGAC CCAAGACATG GTAAATATAT 
GGCTTGTTGT CTATTATATA GAGGTGATGT AGTGCCAAAA GATGTTAATG 
CTGCGATTGC TGCTATTAAA ACAAAAAGAT CTATACAATT TGTAGATTGG 
TGTCCTACCG GTTTCAAGGT TGGTATCAAT TATCAACCAC CAACTGTAGT 
ACCAGGAGGT GACTTAGCTA AAGTTCAAAG AGCTGTTTGC ATGTTAAGTA 
ACACAACAGC TATTGCTGAA GCATGGGCTC GTCTTGATCA TAAATTTGAT 
TTGATGTATG CAAAGAGAGC ATTTGTTCAT TGGTATGTAG GTGAAGGTAT 
GGAGGAAGGT GAATTTTCTG AAGCTAGAGA AGATTTAGCT GCATTAGAAA 
AGGATTATGA AGAGGTTGGT CTAGATAGTG CCGATGCTGG TGAAGACGAA 
GAAGAAGGTC AAGGTGAATA CTAAAAAGTT CGAAGGATGA TTTAAAGAAA 
ACTATAGGAA TTACAAAATT TTAAATAAGA ACTGATTAAT TAAATTTTAG 
ATACATAAAG CATCAATATA CTAATTTTTA AAAAGAGTTA GGTAAAAATG 
ATTCAGTTAG ATCATTTTAA GAATTGTATT GTTTTTTTTA AATTTTATTA 
AGTGAAATTT TAAATAAGAT CAATTTATTA TTAAAATCTA ATTTTATTTT 
TTT

Gene models with high sequence identity

AT1G50010.1 [+] show detail - tubulin alpha-2 chain
E-value: 0; Score: 756.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Tubulin/FtsZ, GTPase domain	6.3e-68
HMMPfam	Tubulin/FtsZ, 2-layer sandwich domain	8.8e-52
superfamily	Tubulin/FtsZ, N-terminal	5.3e-105
superfamily	Tubulin/FtsZ, C-terminal	4.9e-86
PatternScan	Tubulin, conserved site	0
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Alpha tubulin	2.6e-116
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
FPrintScan	Tubulin	4.7e-88
Coil	coiled-coil	0
Gene3D	Tubulin/FtsZ, GTPase domain	1.4e-122
Gene3D	Tubulin/FtsZ, 2-layer sandwich domain	5.8e-51
Gene3D	no description	1.7e-22
HMMSmart	no description	5.1e-80
HMMSmart	Tubulin/FtsZ, 2-layer sandwich domain	1.1e-59
HMMPanther	Alpha tubulin	1.2e-253
HMMPanther	Tubulin	1.2e-253

See the detailed result >

AT1G50010.1

Model type: Protein coding
Short Description: tubulin alpha-2 chain
Curator Summary: Encodes alpha-2,4 tubulin. TUA2 and TUA4 encode identical proteins.
Computational Description: tubulin alpha-2 chain (TUA2); FUNCTIONS IN: structural constituent of cytoskeleton; INVOLVED IN: microtubule-based process, response to salt stress; LOCATED IN: tubulin complex, cytosol, cell wall, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Alpha tubulin (InterPro:IPR002452), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin alpha-4 chain (TAIR:AT1G04820.1); Has 22626 Blast hits to 22529 proteins in 4679 species: Archae - 4; Bacteria - 25; Metazoa - 4391; Fungi - 13400; Plants - 1532; Viruses - 0; Other Eukaryotes - 3274 (source: NCBI BLink).
Link: InterPro Scan - TAIR