K00908

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K00908

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • upturned (PATO:0002031)
    (Rosette leaf::Shape::hyponastic)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K00908

Determination of introduced cDNA(s)

  • J013108A22 - Rice Full-length cDNAs - Original: J013108A22

    full reading [+] show sequence

    Nucleotide sequence: J013108A22 - full reading
    GATCATCCTC ATCGTCGCGC GCACCACTCG CCCGGTCGCC CCATCCCCAC 
    TCTGGCACTC CAGCGCCCAA AAACCTTCGC CTCCGCCTCC GCCATGGGAG
    ACCACCGCGC CACGGCCAAG GTCCGCGTGA ACGGCGCGCC GGCAGAAGCG
    ACGCTCTTCT GCACCGACCC GCCGGGTGCA GCTAATGGCG GCGGCGGAGA
    TGGAGGGGAG CTCCGGTGGC GGTGCGCTGG TGGCGCCGCG GCGGAGCGCG
    TGCTGTCGCT GGACGCGGAC GTGCTCGGGG TCGAGGCCAG GGGTAAGGAG
    GTGGTCGTGA AGGCCTTCGT GCTGCCGGCG GACGCGGCGG CCAGGTCGGT
    GTCCTGCGCC GCGGGTGCGG GGAAGGGAGG AGGAGGACGG AGGAGGCGCA
    GGAGGGATTA CGTGTTCGAG ATGGCCGCCG GCGAGGACGC CGCCGCGGCC
    TGGTGCGACA GGATGAGGGG CTCCCTGGAC TCTCTCGGCC GGCCTAAGAG
    ATTGTTCATC CTGGTCAACC CCTTCGGCGG CAAAAAATGC GGAAAGAAGA
    TTTACGAGGC AGAGATCAAG CCCCTGTTCG AGGCCGCCGG TGTCAACGTC
    ACAATGCAAG AGACCCGGTA TCAGGGGCAC GCCCGGCAGG TGGCGTCTTC
    TCTTGACCTT GCGCGCTACG ATGGCATTGT CTGTGTCAGC GGCGATGGTG
    TCCTCGTGGA GGTAGTAAAT GGAATTCTAC AACGAATGGA TTGGGAGGAA
    GCGATGAAGA TACCGATTGG GGTGGTTCCA GCAGGTACAG GAAATGGCAT
    GGCTAAATCG CTGTTGCATT CTGCCAGTAA GACATACTCG GTTCCAAATG
    CCGTATTTGC CATTATCAGA GGTTACAGTC AGTCCTTGGA TGTATGCACC
    ATTGTGCAGG GAAGGAAGAA ATTTTTCAGT GTCTTGAATA TGACTTGGGG
    TTTGGTGGCT GATATAGATA TTGAGTCTGA GAAGTACAGG TGGATGGGAA
    GTGCACGCTT TGATTTCTAT GCTTTGGTTC GCATAATGAA CTTGAGAAAA
    TACTACGGAA GTATTCAGTA TGTTCCTGCC CCCGGCTATG AGGCATATGG
    AGATGTCGTC AAGCAGGTTG AGAATTGCAC TGTAGAATGC CAAGAACAGA
    TTGGGAAGTC TTTGTGTTCT TATCAAGGTC CTTCCGTTGA GTTTCAAGGT
    TCAGAATGGA GATCCCTTGA TGGTCCATTT GTGTCTATTT GGATCAACAA
    CGTGCAATGG GCTGCTGAGA GTATCATGGC AGCTCCTGGG GCCAAGTTCT
    CAGATGGCTA CATGGATGCA GTCATAGTCA GGGACTGTCC AAAGGCTGAT
    CTTTTAGCCC TACTGATGAA GATGGGCGAT GGGAGTCATG TAAAATCACC
    ATACGTTACG TATCTAAAGG TGAGATGTCT TCGGTTATCA CCAGGCCAGC
    TTGTGGAGAA TCCAAAACGA GGTGGCATAA TAGACGTGGA CGGGGAGGCT
    ATCGCTAGAG GAGAAGGAAC GTACGGCAAA AACCAGAAAC AGGACGTGAT
    GGGTTATGGC CCTTCCATTC AGATGACAGT GCACCGAGCG TTGGCCACAA
    TGTATTGTCC CAAATGACCC CTTTATCTCC TCTCTCTCCC GAGATTGGAG
    TATTTATTCG TGCCAATTGT TTCGCTCTTT GTTAGAAAAA CCTGGTAATC
    TTGAGCAGTA TTCATTAGAA ATATCTTTTG AAAAAAAGAA AACGCTGATA
    CACCATTACA CCTTCGCATG AAACAACATA ACTGGATTAT TTTAACTGG

    Gene models with high sequence identity

    1. AT4G21534.1 [+] show detail - Diacylglycerol kinase family protein
      E-value: 7e-127; Score: 451.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther SPHINGOSINE KINASE-RELATED 5.3e-142
    HMMPanther SPHINGOSINE KINASE 5.3e-142
    HMMPfam Diacylglycerol kinase, catalytic domain 9.3e-26
    HMMSmart Diacylglycerol kinase, catalytic domain 2.2e-08
    ProfileScan Diacylglycerol kinase, catalytic domain 39.821

    See the detailed result >

    AT4G21534.1

    Model type
    Protein coding
    Short Description
    Diacylglycerol kinase family protein
    Computational Description
    Diacylglycerol kinase family protein; FUNCTIONS IN: diacylglycerol kinase activity; INVOLVED IN: activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway; CONTAINS InterPro DOMAIN/s: Diacylglycerol kinase, catalytic domain (InterPro:IPR001206); BEST Arabidopsis thaliana protein match is: sphingosine kinase 1 (TAIR:AT4G21540.1).
    Link
    InterPro Scan - TAIR
  • J013107L04 - Rice Full-length cDNAs - Original: J013107L04

    full reading [+] show sequence

    Nucleotide sequence: J013107L04 - full reading
    GATTTCTCTA ACACACGCTA TTCATTCGGC TGTTTTTTTT ACTCATTTTC 
    ACCATATTTT GCTCTTTCAA TTTATTTTTT TCGGTTGGTG ATTTATTCCC
    GATTACTCTG TGCCGTGTGT CAATTAACCC TCTCTTTCTA AAAATCGAAC
    TCAAAAAAGA ACGGATTCTA ATCTTTTTTT TTTTTTTGTT AAAATTTTGT
    TTATAATTAT TCTTGTTTAG TTTTAAATTT TATTTGTTTA AGTATCTCTA
    AAGATAATCA AAATGCGTGA ATGTATTTCA ATCCATGTTG GTCAAGCCGG
    TGTTCAAATG GGTAATTCTT GTTGGGAATT ATACTGTTTG GAACACAATA
    TTACACCGGA TGGTACAATG CCAAGTGATA CTACATTAGG TAGTAGTGAT
    GATTCATTTA ACACTTTTTT CTCAGAAACT GGAGCTGGAA GGCATGTGCC
    ACGAGCAGTC TACGTTGATT TAGAACCAAC TGTTGTTGAT GAAGTACGTA
    CTGGAGTCTA TAGACAACTA TTCCATCCTG AGCAATTAAT TACTGGTAAA
    GAAGATGCAG CCAATAATTA TGCAAGAGGT CATTATACAA TTGGCAAAGA
    AATTGTAGAC TTAGTTTTAG ATCGTATTCG CAGGCTTTCA GATCAGTGTA
    CTGGATTGCA AGGTTTCTTG ATTTTTCATT CGTTTGGTGG TGGTACAGGT
    TCTGGTTTCA CCAGTTTATT AATGGAGCGT TTAAGTGTTG ATTATGGTAA
    AAAATCTAAA TTAGAATTCG CTATTTACCC AGCTCCACAA GTTTCAACAG
    CAGTAGTCGA ACCATACAAT AGTATTTTAA CAACACACAC AACTCTAGAA
    CATAGTGATT GTGCGTTTAT GGTAGATAAT GAAGCAATCT ATGATATTTG
    TCGTCGTAAT TTGGATATCG AGAGACCAAC CTACACAAAT TTAAATCGTT
    TAATTGGTCA AATTGTATCA TCTATAACTG CTTCTTTAAG ATTCGATGGT
    GCTTTAAACG TTGATTTAAC TGAATTTCAA ACTAATTTGG TACCTTATCC
    ACGTATTCAT TTCCCTCTTG TCACATATGC ACCTGTCATT TCAGCTGAAA
    AAGCTTATCA TGAACAACTA ACTGTTTCCG AAATAACAAA CTCTTGTTTC
    GAACCTTCCA ATCAAATGGT TAAATGTGAC CCAAGACATG GTAAATATAT
    GGCTTGTTGT CTATTATATA GAGGTGATGT AGTGCCAAAA GATGTTAATG
    CTGCGATTGC TGCTATTAAA ACAAAAAGAT CTATACAATT TGTAGATTGG
    TGTCCTACCG GTTTCAAGGT TGGTATCAAT TATCAACCAC CAACTGTAGT
    ACCAGGAGGT GACTTAGCTA AAGTTCAAAG AGCTGTTTGC ATGTTAAGTA
    ACACAACAGC TATTGCTGAA GCATGGGCTC GTCTTGATCA TAAATTTGAT
    TTGATGTATG CAAAGAGAGC ATTTGTTCAT TGGTATGTAG GTGAAGGTAT
    GGAGGAAGGT GAATTTTCTG AAGCTAGAGA AGATTTAGCT GCATTAGAAA
    AGGATTATGA AGAGGTTGGT CTAGATAGTG CCGATGCTGG TGAAGACGAA
    GAAGAAGGTC AAGGTGAATA CTAAAAAGTT CGAAGGATGA TTTAAAGAAA
    ACTATAGGAA TTACAAAATT TTAAATAAGA ACTGATTAAT TAAATTTTAG
    ATACATAAAG CATCAATATA CTAATTTTTA AAAAGAGTTA GGTAAAAATG
    ATTCAGTTAG ATCATTTTAA GAATTGTATT GTTTTTTTTA AATTTTATTA
    AGTGAAATTT TAAATAAGAT CAATTTATTA TTAAAATCTA ATTTTATTTT
    TTT

    Gene models with high sequence identity

    1. AT1G50010.1 [+] show detail - tubulin alpha-2 chain
      E-value: 0; Score: 756.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Tubulin/FtsZ, GTPase domain 6.3e-68
    HMMPfam Tubulin/FtsZ, 2-layer sandwich domain 8.8e-52
    superfamily Tubulin/FtsZ, N-terminal 5.3e-105
    superfamily Tubulin/FtsZ, C-terminal 4.9e-86
    PatternScan Tubulin, conserved site 0
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Alpha tubulin 2.6e-116
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    FPrintScan Tubulin 4.7e-88
    Coil coiled-coil 0
    Gene3D Tubulin/FtsZ, GTPase domain 1.4e-122
    Gene3D Tubulin/FtsZ, 2-layer sandwich domain 5.8e-51
    Gene3D no description 1.7e-22
    HMMSmart no description 5.1e-80
    HMMSmart Tubulin/FtsZ, 2-layer sandwich domain 1.1e-59
    HMMPanther Alpha tubulin 1.2e-253
    HMMPanther Tubulin 1.2e-253

    See the detailed result >

    AT1G50010.1

    Model type
    Protein coding
    Short Description
    tubulin alpha-2 chain
    Curator Summary
    Encodes alpha-2,4 tubulin. TUA2 and TUA4 encode identical proteins.
    Computational Description
    tubulin alpha-2 chain (TUA2); FUNCTIONS IN: structural constituent of cytoskeleton; INVOLVED IN: microtubule-based process, response to salt stress; LOCATED IN: tubulin complex, cytosol, cell wall, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Alpha tubulin (InterPro:IPR002452), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin alpha-4 chain (TAIR:AT1G04820.1); Has 22626 Blast hits to 22529 proteins in 4679 species: Archae - 4; Bacteria - 25; Metazoa - 4391; Fungi - 13400; Plants - 1532; Viruses - 0; Other Eukaryotes - 3274 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR