K02025

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K02025

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
whole plant flowering stage (PO:0007016)
  • premature (PATO:0000694)
    (Adult plant::Flowering::early)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K02025

Determination of introduced cDNA(s)

  • J033058N01 - Rice Full-length cDNAs - Original: J033058N01

    full reading [+] show sequence

    Nucleotide sequence: J033058N01 - full reading 
    GATAGAGCAC AGTCCTCTCC TCTTCCCCGT GCTCCCCATT TCTGCTTCTT 
    TTCCCACAAA TTTCTCTCTC TATCTCAGAA AGGATTCGGG AGACTTGAAG 
    AGAGGAGCTC GAAGCGGGAC AGGAGAGAGA GGCTAATTAT AGAAGTACAC 
    GAAGAAAAAT GGTGCCTGGA CCAAAGTGAG ACCATCTCAT AATGCTAATG 
    CTTTTGCGAC AAATGGAAGA TCATCGAGCC CATACTACGC CCAACTATGA 
    TTTTTTCTCT GGAGATTATC AGATGAAGCA GTTAGGTCAT AAGATGTATG 
    ATCAAGATTC CCCGTCAAGC GATTCTGGAC AGTCACACCA AGAAGAATCT 
    GCCATGAATG ATAGCAGTCC AAATGAGCGA CATACCTCAA CACAATCTGA 
    CAATGATGAT GGTCATCAGA TGCCAGATCA GGACAAAACA AAGTCAGTAT 
    CATCGTTGGG GAATCCAGGA GCTTTGCCTC CAAAGCTCAA CTATAGCCAA 
    TCCTTTGCTT GTATTCCTTA TACAGCTGAT GCATACTATG GTGGGGTCTT 
    GACTGGATAT TCTTCACATG CAATTGTTCA TCCCCAGCAA AATGGTACAG 
    CTAACTCCCG GGTGCCGTTG CCTGTTGAGC CTGCAGCAGA AGAGCCAATA 
    TTTGTAAACG CAAAGCAGTA CCATGCAATT CTTAGGAGGA GACAGATACG 
    AGCTAAATTG GAGGCCCAAA ATAAGTTGGT GAAAGGTCGG AAGCCATACC 
    TCCATGAATC TCGGCATCGC CATGCCATGA AGCGAGCTCG TGGATCTGGA 
    GGGCGCTTCC TCAACACAAA GCAGCTCGAG GAGCAGAAGC AGCAACAGGA 
    GGAGGAGGCC GCATCCGGTG GCGCGAGCTC TGGCAATAGG ACATGCCTTC 
    AGAATGGCAC CGGTAGCGCG CCTTCAGCTT CCTCTCCCTC TGAAATCGCG 
    AGCGTCTCAA CCAGCAGAGA ATTCCTTGGT AACCATGAGC AGAGCCACTT 
    CCCCTCAGCT GGCTTCCTTC CCACAATGAG CTTCAGAGCG CAGAATGGCG 
    GAGATGGGAA GCTGGTCGCG AACGCCATTC ACCAGCGCGT TTCCATGATG 
    AGGTGAGGTG AAGAGGGGTG AAGTGGGTTC AGCTCAGCAC CATCACTTTG 
    GCTCTCTCCC TGCTGGTGGC AATTCATCCT TGGCTTATGA AAATACCTTA 
    TGGGAAAAAA AAAAGTAGTT TCATCCTCAA ATGGACCAAA CATGTGTGGC 
    TCCATTTTAA GTTTTCCTGT AACTAAAAGC ACTGCAATGA TCAACTGACT 
    GTTGGTTCTG TTTGTGGATG CTTATATCTG TACTTGCTGT ATCTAATACT 
    GAACTCTAAG TTGAAACCCT GAAACTGATG GTCCCCGTGA ATATTGTAGC 
    TTGCATTTTA TC

    Gene models with high sequence identity

    1. AT1G54160.1 [+] show detail - nuclear factor Y, subunit A5
      E-value: 5e-27; Score: 119.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart CCAAT-binding transcription factor, subunit B 2.5e-37
    HMMPanther TRANSCRIPTIONAL ACTIVATOR PHP2-RELATED 1.1e-62
    HMMPanther CCAAT-binding transcription factor, subunit B 1.1e-62
    HMMPfam CCAAT-binding transcription factor, subunit B 3e-30
    FPrintScan CCAAT-binding transcription factor, subunit B 1.8e-24
    FPrintScan CCAAT-binding transcription factor, subunit B 1.8e-24
    ProfileScan CCAAT-binding transcription factor, subunit B 38.238
    PatternScan CCAAT-binding factor, conserved site 0

    See the detailed result >

    AT1G54160.1

    Model type
    Protein coding
    Short Description
    nuclear factor Y, subunit A5
    Curator Summary
    Encodes a member of the CCAAT-binding transcription factor (CBF-B/NF-YA) family. Expression is upregulated in response to ABA and drought. This regulation appears to be mediated by MIR169A which is downregulated in response to drought. NFYA5 is a target of MIR169A. Loss of function mutations are hypersensitive to drought.
    Computational Description
    "nuclear factor Y, subunit A5" (NF-YA5); FUNCTIONS IN: sequence-specific DNA binding transcription factor activity, specific transcriptional repressor activity; INVOLVED IN: response to water deprivation, negative regulation of gene-specific transcription, regulation of transcription, DNA-dependent; LOCATED IN: CCAAT-binding factor complex, nucleus; EXPRESSED IN: 10 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis, C globular stage, petal differentiation and expansion stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: CCAAT-binding transcription factor, subunit B (InterPro:IPR001289), CCAAT-binding factor, conserved site (InterPro:IPR018362); BEST Arabidopsis thaliana protein match is: nuclear factor Y, subunit A6 (TAIR:AT3G14020.1); Has 683 Blast hits to 683 proteins in 160 species: Archae - 0; Bacteria - 0; Metazoa - 143; Fungi - 133; Plants - 381; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR