K02143

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K02143

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • variegated (PATO:0000335)
    (Rosette leaf::Color::variegated)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Original: K02143

Determination of introduced cDNA(s)

  • J013122I07 - Rice Full-length cDNAs - Original: J013122I07

    full reading [+] show sequence

    Nucleotide sequence: J013122I07 - full reading 
    TTTCTTTTTT TTTTTAGTGA GTAAAGTTAA GCAGTTTGAA AATGCTGCCG 
    GATAATCTCT TTACACCCAA TTCATTTATG AGTGCTCATC TGCGAATGAC 
    CTGACCAAAG CAAAGCCTTT TCATTTTGTC TTCTGACAGT AAATACAGCA 
    CGTGACAAGA TAACAAAATC ATCAAACTTC TAAACGAAAC CTTGCAAAAA 
    CAATGACAAT TGACAAAGTG AGAAGCAAAG CTCAACGTGC CTAGGCAGCG 
    GTTTGCAATC ACCGTCTTCC CCTGCCCTTG CTCATGAAGT GGTGGCGCTT 
    CAACCTCTTC TTCCGGATCA CCACGGAGGA CTTGATGTGC TCCGCGAACG 
    GCATGCCAAG GAGGGTGAGC AGCCTGAGAG CCTCGTTGTC GGTCTTCGCG 
    GTGGTGACGA TGCAGACGTC CATTCCATTC TTCTTCCCAC CGACCTCGTA 
    CGGGATCTCC GGGAACACGC CCTGATCACG AAGGCCTATG GTGAAGTTGC 
    CATGGCCATC AAAGCTGTTG GGGTTGACAC CAAGGAAGTC CATGGTCCTA 
    GGAAGCCCAA GGTTGATGAG CCTATCCAGG AAGTTGAACA TTACCCTGCC 
    GCGGAGCGTA ACGGCAATAC CAATGGTGTT CCCCTCGCGG ATCTTGAAAC 
    TGGCGACGGA CTTCTTGGCC TTCGTCTTGA CGGGCCACTG GCCGGTGATC 
    ATGGCGAGGT CCTTCATGGC GGATTCGAGC CCCTTGGAGT TACCGGCTTC 
    AGCCCCCAAC CCGCAATTCA CTACAATCTT CTCAACCTTG GGCACCTGGT 
    GGACATTGGT GTAGCCGAAC TCCTCGGTGA TGAGGGGGAC CACCTTCTGG 
    TCGTATACAG CCTTGAGGCG GTGCACCTTC TGTGCCTCGG TGGGGTCGAC 
    GAGGACGATG CCCGAAGGCG AGGTCGGCGG CGGCGCCGGC TTGGGTGGCG 
    CGTCGGCCGC GGCGGAGGCG GCGACGCGGA GGGCTCGGCG AGGAGGCGGG 
    GAGCGGAGGA GGAGGCGGAC GTTACGGGAG GAGGAGGCGG TGGTGGTGAC 
    GGGGAAGGGG GCCGGCGAGG ACGGCAGGGT CACAGCCGTG GCCGCCATTG 
    GAGCGTGCGC GGAGCAGAGG CTGCTCAGGA AGGGCGGCGC CGCTGCCGCC 
    GGCGGCGGGC TCACCGTCGA GGGCTTCCAC CGCTGGCTGT GCAGCGACGC 
    CAACGCTGCA CTGGATCCTC AGAAACGGGT ATATCAAGAC ATGGGATTGC 
    CTCTGTCACA CTACTTCATC TACACGGGGC ACAACTCATA CCTGACGGGG 
    AACCAGCTGA GCAGCGGGTG CAGCGAGGTG CCCATCGTGA AGGCCCTCCA 
    CGACGGCGTC CGTGTCATCG AGCTCGACCT CTGGCCCAAC GCTGCCAAGG 
    ACGCCGTCGA GGTCCTCCAC GGCAGGACGC TGACATCGCC AGTGGGTCTG 
    ATGAAATGCC TTGAGGCCAT CAGGGAGTAC GCGTTCGTCG CCTCCCCTTA 
    CCCCGTCATC CTCACCCTCG AAGATCACCT CACTCCCGAC CTCCAATCCA 
    AAGTCGCCAA GATGATCAAG GAGACGTTCG GTGACATGCT CTACGTCTCT 
    GAAACTGAGA ACATGGCGGA ATTCCCTTCG CCGGACGAGC TCAAGGGGAA 
    GATCATCGTG TCGACGAAGC CGCCCAAGGA GTACCTCCAG ACCAAGAACG 
    ACGCCGACGC CGACGAGGCC GGCGTCTGGG GCGAGGAGAT CACCGACGAC 
    AAGGTGGCGG CGACGGCGAT GACGACAGAA GAGAAATGCG CGGCGGCGGA 
    GGAAGCAGTC GCCGCCGCCG CCGTCGACGA GGAGATGCAG GAGGCCGAGA 
    CGGACAAGAA GACGCAGCAC GGGGTGGACA ACGAGTACAG GCGCCTCATC 
    GCCATCCCTC TGACAAGAAG AAAGCACGAC ATGGATCAGG ACCTCAAGGT 
    CGATCCCGAC ATGGTGACGC GCCTCAGCCT CGGGGAGAAG GCCTACGAGA 
    AGGCCATTGT CACCCATGGA GCTCACATTA TCAGGTTTAC GCAGAGGAAA 
    TTGCTGAGGA TATTCCCTCG GTCGACGCGC ATTACGTCTT CGGATTATAA 
    TCCTTTGATG GGTTGGAGGT ATGGAGTTCA GATGGTTGCA GCAAACATGC 
    AGGGCCATGG AAGAAAGCTG TGGCTGACTC AGGGGATGTT CCGAGCAAAC 
    GGCGGTTGCG GTTACGTCAA AAAGCCTGAT ATTCTGATGA ACAACGATCC 
    AGATAAACTG TTTGATCCTA CATCCAAACT ACCAGTAAAG ACAAGACTAA 
    AGGTGACGGT GTACATGGGA GACGGATGGC GATTCGACTT CCGCAAGACA 
    CATTTCGACA AATGCTCACC CCCAGACTTC TATGCAAGGG TGGGTATAGC 
    CGGAGTAGAA GCAGACACGA GGATGGAGCA GACGAAGGTG AAGATGGACA 
    CGTGGATTCC GGCATGGGAT CACGAGTTCG AGTTCCCGTT GAGCGTGCCG 
    GAGCTGGCGC TGCTCCGGGT GGAGGTGCAC GAGTCCGACA ACCACCAGAA 
    GGACGACTTC GGCGGGCAGA CCTGCCTGCC GGTGTGGGAG CTCCGGCGAG 
    GCATCCGCTC CGTCAGGCTC TGCGACCACC GCGGCGAGCC CCTCCGCTCC 
    GTCAAGCTCC TCATGCGCTT CGACTTCACC TGACTACTCC ATCTTCTTCT 
    TCATCATCGT CATCGTCTTA GTAGTAGTAG TTCCATGTAT GATATCATGA 
    GGGATGAAGC ATTGATCGTA CTTTCTCCGT CCCGAAATAT AGCAATGTAG 
    GATTAGATGG AAACATCTTA ATACTACGAA TCTGGATATG TCCTATCTGG 
    TTCTGGTTTG CTATGTTTTA GGACGAATAT AGTACATAAA ATTTTAGAAG 
    TTTTGTTTTA AAAAAG

    Gene models with high sequence identity

    1. AT3G08510.2 [+] show detail - phospholipase C 2
      E-value: 7e-162; Score: 568.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Phospholipase C, phosphatidylinositol-specific , X domain 5e-67
    HMMSmart Phospholipase C, phosphatidylinositol-specific, Y domain 4.1e-45
    HMMSmart C2 calcium-dependent membrane targeting 1e-11
    HMMPfam Phospholipase C, phosphatidylinositol-specific , X domain 1.2e-48
    HMMPfam Phospholipase C, phosphatidylinositol-specific, Y domain 3.3e-26
    HMMPfam C2 calcium-dependent membrane targeting 1.4e-12
    HMMPfam Phospholipase C, phosphoinositol-specific, EF-hand-like 7e-06
    HMMPfam Ribosomal_L5_C 1.4e-30
    HMMPfam Ribosomal_L5 3.7e-21
    PatternScan Ribosomal protein L5, conserved site 0
    superfamily PLC-like phosphodiesterase, TIM beta/alpha-barrel domain 9.7e-112
    superfamily C2 calcium/lipid-binding domain, CaLB 4.5e-24
    superfamily Ribosomal protein L5 1.8e-66
    Gene3D PLC-like phosphodiesterase, TIM beta/alpha-barrel domain 1.5e-99
    Gene3D no description 5.7e-35
    Gene3D Ribosomal protein L5 7.8e-64
    HMMPanther 50S RIBOSOMAL PROTEIN L5 3.4e-193
    HMMPanther Ribosomal protein L5 3.4e-193
    HMMPanther PHOSPHOLIPASE C 7.5e-290
    HMMPanther PHOSPHOLIPASE C 7.5e-290
    FPrintScan Phospholipase C, phosphoinositol-specific, C-terminal (PLC) 4.8e-40
    FPrintScan Phospholipase C, phosphoinositol-specific, C-terminal (PLC) 4.8e-40
    FPrintScan Phospholipase C, phosphoinositol-specific, C-terminal (PLC) 4.8e-40
    FPrintScan Phospholipase C, phosphoinositol-specific, C-terminal (PLC) 4.8e-40
    FPrintScan Phospholipase C, phosphoinositol-specific, C-terminal (PLC) 4.8e-40
    FPrintScan Phospholipase C, phosphoinositol-specific, C-terminal (PLC) 4.8e-40
    ProfileScan C2 membrane targeting protein 12.691
    ProfileScan Phospholipase C, phosphatidylinositol-specific , X domain 42.969
    ProfileScan Phospholipase C, phosphatidylinositol-specific, Y domain 33.415

    See the detailed result >

    AT3G08510.2

    Model type
    Protein coding
    Short Description
    phospholipase C 2
    Curator Summary
    Phosphoinositide-specific phospholipase C (PI-PLC), catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-trisphosphate and diacylglycerol.
    Computational Description
    phospholipase C 2 (PLC2); FUNCTIONS IN: phospholipase C activity; INVOLVED IN: signal transduction, intracellular signaling pathway, lipid metabolic process; LOCATED IN: plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C, phosphoinositol-specific, EF-hand-like (InterPro:IPR015359), Phospholipase C, phosphatidylinositol-specific , X domain (InterPro:IPR000909), C2 calcium/lipid-binding domain, CaLB (InterPro:IPR008973), Phospholipase C, phosphoinositol-specific (InterPro:IPR001192), Phospholipase C, phosphatidylinositol-specific, Y domain (InterPro:IPR001711), PLC-like phosphodiesterase, TIM beta/alpha-barrel domain (InterPro:IPR017946), C2 membrane targeting protein (InterPro:IPR018029), C2 calcium-dependent membrane targeting (InterPro:IPR000008); BEST Arabidopsis thaliana protein match is: Phosphoinositide-specific phospholipase C family protein (TAIR:AT3G55940.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR