K03134

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K03134

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K03134

Determination of introduced cDNA(s)

  • J013043E22 - Rice Full-length cDNAs - Original: J013043E22

    full reading [+] show sequence

    Nucleotide sequence: J013043E22 - full reading 
    GCCTTCTTCC GATCTCCCAC CGTCGCCTGG GCGCCTGGCG GTTTGCTCCA 
    CTCCATAGGC GGCCATCGTC TTCTCAACCA GAAAGCTTCT GGCTGTACAG 
    CTTGGCGGGA TACAAGACGA CGGTCTGCCG TCGGCGATCC GCAGTCGCCG 
    GAGCTGAAGG TGGAAGCGGC CAACACGGCA GTCCGTGCAG CCGCTTGAGA 
    ATTTTTTGTC ATGGCAGTGA AAAGATGCGC AGAAGTGCCG ATATCTGCTA 
    GGCCGGGAAA GATGATGCGG ATTGCTATTA AGTCTTGGTC AAGGTTCTTG 
    CCCCCTTACA TCCCTGACGA TGTGATCTTC GACATTCTGT TACGGCTGCC 
    ATCCAAATCT CTTATCCGAT TTAAGTCCGT GTGTAAGGCT TGGCACGCCA 
    TAATATCCAA CCCCTGTTTC ATCAGTGCTC ACCTCGAGTG CTCTAAGCAG 
    AAGCCATCGA TATTTATGGT CCCTGGTGTT TACGAGAAGC AGAATAATGG 
    CGAGAATACT TCCTTCCTGA TGGGCCTCTA TCAGTATCAG GGTGGTAATA 
    TAATGGAACA AATCCATGTA CAAGACTTTC CACAAGGCAT TGGTACATGG 
    AGTCGTCCGA TCCACTGCAA TGGCATGCTC CTCATTTCCA CCATGAACCA 
    CGAAATGATT GTCTGCAACC CATCAACTAG AGAGATTGTC TCCCTGCCAA 
    AAGGGAGTTA TAACCTTCAT GCGGGCCCAA GGGCTGGATT TGGGTTTGAC 
    CCTCACAGCA ACAAGTATAA GGTGGCTAGA TTTTTCTACC AGAGGGACGA 
    TGACACATCA GAATTGGTCT GCAAGTTTGA AGTGCTTACT CTAGGCACTA 
    ATTTGTGGAG GCAAACTGAG GATCCGCCAT ATCCAATTAG TGGGTTAACT 
    CCTGTTCATG TGAAAGGCGC CATCTACTGG ATGGTTAATA CGCCACTTTG 
    CCCTGATCCA CCCAATGCAT TTCTTAGGTT CTGTTTGACT AACGAGAAGT 
    TTAGTCTGCT TCAGTATCCT CCATGTAATC TAAAGCCTAC CCGTTTCATA 
    GAAGTGGAGG GTGAACTATG CTGTGCTTGC TTTTGCAGTC AGGTGAGCGC 
    GTTGAAGATC TGGACCTGCA ATTATGCACA GAACCCTGAA TGGACTCAGC 
    GCTGCACTGT ACAGATTCCA CCTGATATAG TTGTGAATAA TCCTGTAGCA 
    AGGCCTCCTA TAGTTTTTCT TCATGGAAAA AAGCTGCTAC TGACATGGAA 
    TCAGGTCTAC CAGTATGACA TTCAAACCTG TAGAATGGAA AAGATAGCTT 
    CAGGGGTTGA AGACTTCACG TGCTATGATC CAAGGAACAA TAAGTACTGG 
    GCATATCTGG AGAAAGAAGT AACGGATATG CATCTATTTA ATTATGCTGA 
    GAGTCTAGTT CCGATTAGAG AATTTTGAGA AGTCACTTCA TTTTTTGTTA 
    AGATGTGGAG CCTTGCATTT CAGGCCCAAA GAGTCCTCAT GACCGTGTCC 
    CTTTGAAGGA AATGAAATCA GATTGGCATG CTTGCCTGGA CAGCAATTTC 
    AAGGTAACTA AGGATAACCT TTGCATTATA AAGTTTAAAA TGTAGTGGCA 
    TTGCTCATCA AAATACACCC CAAAAAACAC ACTAGGGTTT TGCAGTCCCA 
    ACGGAGTACC ATGATAAAGT TGTGAAATTT TACTTTCAAG GACAACCTGC 
    TGAAATCAAA CAGTGTTGTT CTTGCAGCAA TATGCAGTTA CACAAACACA 
    TCAAATCCCA GCGTTATAAT TGGTGCTGGC CTAGTCGCAA AGAAAGCCTG 
    TGAATTGGGC CTTGAGGTTT GCATGTTCGT TCATATATGT AACTAGTGTA 
    TTGTGTCGTG TGTTTGTGCG ACTATAACCT CCCTCTCTGT AAATATGTAT 
    AATAATTAAC TTAATTTGTT AATAACATTT AATAATTTAA ATTAGTATTT 
    CCTCCTTTTA TAAGATAATT TGTTTATTCT TCACTTGAAT TTGAATAGAT 
    GTTCAGAGAA CATATTTAGC AGGAAATCTA GTATGTATTT TTTTAATTTT 
    ACAAAAAAGA CCATTTATTT AAATAAGTTG TATTGAAATG TCAACTGTAC 
    AATTTTTTTA GCAAGTTTTC TGTTAGTTGC TAGAATTAAA ATTAATTATA 
    TGATTATACT TTTAAACTGG TCTCGATTTT TAGTTCTTTA TGGTCAGAAT 
    GTTAATTTAC AATGGTTTAA ATTATCGGTT GCGGCTGGTG GTGGACTGCC 
    ACTGTTTCTC GGACTTCTCT CTTCACAGGA AGTGGTTATT GCCTTTCGGC 
    CACATATTTG CCAACAATAA ATTCTTTTAT GAAGAATTTA GTATGTACAA 
    TCTCATGCAT CGGGGTAAAA CTTTTCCAGG TTATAAATTT ACAACCACTA 
    AAATATAGAT AAAAATGGGC ATGCACCTAA ATCAACCAAA CAGTTCATAC 
    CAGAAATAAA AATTAAGGAA CCTAACTAAT TGCATTAATC ATTGACGGAA 
    GATGCAGATT TCAGAAATGC CAAGCTCTTT CATTTGGTTG TGATGCAATC 
    CAGGAAGAAC TATTAAACAT TAGAAATAAT CCATTGCAAT ATAGGAAATT 
    CGTGTTTACT GGCGAACTTC TACTGCTTGG TATAATTCCA AATTAATTTG 
    GTTGCTATGG ACCCGTCAGA ACAGTTTTTC GTCGGATTCC AGATTTTATG 
    GATACTGGAG GTCTACAATT GCTTATTAAT TAGCTCGATA GAATCAACCA 
    CTACAATCTG CACATAGAGG GTGTGAGCTT CCATGTTAAG TGCTTTACAT 
    AGGCTGATTT AAAAACACTT GTAATGGAAC TTATGCAGCT GTTGCTGTCA 
    TTAGATGATC CTTAACACTT TTAGCTTCCA GAATGAAAAG GATTAAGTAT 
    TTAAACTATG GATGAGGGCT TCATAACCTG TATGACAACA GTGGATTTCC 
    ACTAAGATGC ATGCCTACAT TCAAAATCTG AGTTAGAAAA GAAAATTTAG 
    CAATTTGATA CATAATACAT ACCCTTGGAT TAGGATATTC TTATGAGTAC 
    TTTACCATAT TCACTAAACT CCAGGAACAC TTCATATTTA AGAAAATGCA 
    GTATGAGATA TGTAAGTGCA ATCAGCTACT GTGCCTTTTG TCACAGGACC 
    TTCTAGGTGC ACACAATTAG TACCAGTGTG CTCATGCTGC AGATTGCTAT 
    GAATGCTAAT TGGATTAACA CCATGAGTTC ATAAGTAATA AACCGTTTGC 
    AGTGGCATT

    Gene models with high sequence identity

    1. AT4G26970.1 [+] show detail - aconitase 2
      E-value: 1e-26; Score: 38.50

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam F-box domain, cyclin-like 8.5e-07
    HMMPfam F-box associated domain, type 1 2e-06
    ProfileScan F-box domain, cyclin-like 8.941
    superfamily F-box domain, Skp2-like 1.7e-11
    HMMSmart F-box domain, cyclin-like 4.3e-08
    HMMPanther Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase 1.5e-14
    HMMPanther Aconitase-like core 1.5e-14
    HMMTigr F-box associated interaction domain 5.4e-19

    See the detailed result >

    AT4G26970.1

    Model type
    Protein coding
    Short Description
    aconitase 2
    Curator Summary
    Encodes an aconitase that can catalyze the conversion of citrate to isocitrate through a cis-aconitate intermediate, indicating that it may participate in the TCA cycle and other primary metabolic pathways. The protein is believed to accumulate in the mitochondria and the cytosol. It affects CSD2 (At2g28190 - a superoxide dismutase) transcript levels and may play a role in the response to oxidative stress. One member of the family (ACO1 - At35830) was shown to specifically bind to the 5' UTR of CSD2 in vitro.
    Computational Description
    aconitase 2 (ACO2); FUNCTIONS IN: aconitate hydratase activity, copper ion binding; INVOLVED IN: response to oxidative stress, response to cadmium ion, isocitrate metabolic process, citrate metabolic process; LOCATED IN: cytosol, mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 17 growth stages; CONTAINS InterPro DOMAIN/s: Aconitase family, 4Fe-4S cluster binding site (InterPro:IPR018136), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha (InterPro:IPR001030), Aconitase A/isopropylmalate dehydratase small subunit, swivel (InterPro:IPR000573), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2 (InterPro:IPR015932), Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase (InterPro:IPR015934), Aconitase/3-isopropylmalate dehydratase, swivel (InterPro:IPR015928), Aconitase-like core (InterPro:IPR015937), Aconitase/iron regulatory protein 2 (InterPro:IPR006249), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 (InterPro:IPR015931); BEST Arabidopsis thaliana protein match is: aconitase 3 (TAIR:AT2G05710.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR